插入诱变诱导的yezoensis (Rhodophyta)孢子发育突变体的分离和鉴定。

IF 2.8 3区 生物学 Q1 MARINE & FRESHWATER BIOLOGY
Hikari Izumi, Yuji Nakai, Toshiki Uji, Kaz Nagaosa, Satoru Fukuda, Hiroyuki Mizuta, Naotsune Saga
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引用次数: 0

摘要

阐明孢子发育的机理对了解海藻的繁殖策略和提高海藻的养殖水平至关重要。在这项研究中,我们从插入诱变产生的遗传转化体中分离出一个具有孢子发育缺陷的yezoensis (Rhodophyta)突变体。我们描述了突变表型并确定了包含突变的基因组区域。突变体在生命史的每个相关阶段都产生孢子(原始孢子、合子孢子和贝壳孢子)。在光镜下,野生型和突变型菌株在任何阶段释放孢子的外观没有明显差异;然而,与野生型相比,突变体表现出孢子释放减少的趋势。另外,与野生型相比,突变体释放的原孢子从孢子囊中释放出来后仍保持圆形,不能附着在基质上,不发芽,在标准培养4天后没有细胞壁发育。合子孢子和贝壳孢子也观察到类似的结果。这些结果表明,该突变体的主要特征是孢子释放不良,释放的孢子不能发芽。全基因组测序显示外源DNA被引入突变体的核基因组,并提示突变体基因组中存在重复、缺失和反转。值得注意的是,在插入的突变体基因组侧翼检测到约13 kb的缺失,该区域包含一个植物同源结构域,这表明含有同源结构域的蛋白质可能调节叶芽草孢子的发育。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Isolation and characterization of a spore development mutant in Pyropia yezoensis (Rhodophyta) induced via insertional mutagenesis.

Mechanistic elucidation of spore development is essential for understanding reproductive strategies and improving seaweed cultivation. In this study, we isolated a mutant with spore development defects in Pyropia yezoensis (Rhodophyta) from among genetic transformants generated via insertional mutagenesis. We characterized the mutant phenotype and identified the genomic region containing the mutation. The mutant produced spores (archeospores, zygotospores, and conchospores) at every relevant stage of the life history. No evident differences were observed in the appearance of the released spores at any stage under light microscopy between the wild-type and mutant strains; however, the mutant exhibited a trend of reduced spore release compared with the wild-type. Additionally, the archeospores released by the mutant remained round after being released from the sporangium, failed to adhere to the substratum, did not germinate, and showed no cell wall development after 4 days of standard culture, in contrast to the wild-type. Similar results were observed for the zygotospores and conchospores. These findings indicate that the main characteristic of this mutant was poor spore release, and the released spores failed to germinate. Whole-genome sequencing revealed the introduction of exogenous DNA into the nuclear genome of the mutant and suggested the presence of duplications, deletion, and inversion in the mutant genome. Notably, a deletion of approximately 13 kb was detected in the mutant genome flanking the insertion, and this region contained a plant homeodomain, suggesting that homeodomain-containing proteins may regulate spore development in P. yezoensis.

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来源期刊
Journal of Phycology
Journal of Phycology 生物-海洋与淡水生物学
CiteScore
6.50
自引率
3.40%
发文量
69
审稿时长
2 months
期刊介绍: The Journal of Phycology was founded in 1965 by the Phycological Society of America. All aspects of basic and applied research on algae are included to provide a common medium for the ecologist, physiologist, cell biologist, molecular biologist, morphologist, oceanographer, taxonomist, geneticist, and biochemist. The Journal also welcomes research that emphasizes algal interactions with other organisms and the roles of algae as components of natural ecosystems. All aspects of basic and applied research on algae are included to provide a common medium for the ecologist, physiologist, cell biologist, molecular biologist, morphologist, oceanographer, acquaculturist, systematist, geneticist, and biochemist. The Journal also welcomes research that emphasizes algal interactions with other organisms and the roles of algae as components of natural ecosystems.
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