Samira Karzani, Aghil Sharifzadeh, Bahar Nayeri-Fasaei, Ali Reza Khosravi, Jalal Hassan, Aram Sharifi, Ali Pourshaban Shahrestani
{"title":"橄榄苦苷对斑马鱼胚胎中白色念珠菌和金黄色葡萄球菌粘附的影响及其毒性。","authors":"Samira Karzani, Aghil Sharifzadeh, Bahar Nayeri-Fasaei, Ali Reza Khosravi, Jalal Hassan, Aram Sharifi, Ali Pourshaban Shahrestani","doi":"10.18502/ijm.v17i2.18390","DOIUrl":null,"url":null,"abstract":"<p><strong>Background and objectives: </strong>The rising prevalence of antibiotic resistance and biofilm-associated infections poses significant challenges in clinical settings. This study investigates the antimicrobial and anti-adhesive properties of oleuropein, a compound derived from olive leaves, against <i>Candida albicans</i> and <i>Staphylococcus aureus.</i></p><p><strong>Materials and methods: </strong>This study was conducted on <i>Candida albicans</i> (fluconazole-resistant/susceptible) and <i>Staphylococcus aureus</i> (methicillin-resistant/susceptible). The antifungal, antibacterial, anti-adhesion, and cell surface hydrophobicity (CSH) effects of oleuropein were evaluated. The impact of oleuropein on germ tube formation (GTF) in <i>C. albicans</i> was assessed. Finally, the toxicity of oleuropein was evaluated in zebrafish embryos.</p><p><strong>Results: </strong>Oleuropein exhibited MIC values of 10 mg/ml for <i>C. albicans</i> and 5 mg/ml for <i>S. aureus</i>. It significantly (P< 0.05) reduced the adhesion of both microorganisms in a dose-dependent manner, with inhibition percentages of 78.43% and 75.91% for <i>C. albicans</i> and <i>S. aureus</i>, respectively. Additionally, oleuropein reduced the CSH of <i>C. albicans</i>, indicating its potential to interfere with adhesion mechanisms. In addition, oleuropein exhibited inhibition of GTF in <i>C. albicans.</i></p><p><strong>Conclusion: </strong>Oleuropein demonstrates significant antimicrobial and anti-adhesive properties against <i>C. albicans</i> and <i>S. aureus,</i> indicating its potential as a therapeutic agent for preventing biofilm-related infections. However, careful dosage management is crucial due to its observed toxicity at higher concentrations.</p>","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"17 2","pages":"312-320"},"PeriodicalIF":1.7000,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12053420/pdf/","citationCount":"0","resultStr":"{\"title\":\"Impact of oleuropein on <i>Candida albicans</i> and <i>Staphylococcus aureus</i> adhesion and its mediated toxicity in Zebrafish (<i>Danio rerio</i>) embryos.\",\"authors\":\"Samira Karzani, Aghil Sharifzadeh, Bahar Nayeri-Fasaei, Ali Reza Khosravi, Jalal Hassan, Aram Sharifi, Ali Pourshaban Shahrestani\",\"doi\":\"10.18502/ijm.v17i2.18390\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background and objectives: </strong>The rising prevalence of antibiotic resistance and biofilm-associated infections poses significant challenges in clinical settings. This study investigates the antimicrobial and anti-adhesive properties of oleuropein, a compound derived from olive leaves, against <i>Candida albicans</i> and <i>Staphylococcus aureus.</i></p><p><strong>Materials and methods: </strong>This study was conducted on <i>Candida albicans</i> (fluconazole-resistant/susceptible) and <i>Staphylococcus aureus</i> (methicillin-resistant/susceptible). The antifungal, antibacterial, anti-adhesion, and cell surface hydrophobicity (CSH) effects of oleuropein were evaluated. The impact of oleuropein on germ tube formation (GTF) in <i>C. albicans</i> was assessed. Finally, the toxicity of oleuropein was evaluated in zebrafish embryos.</p><p><strong>Results: </strong>Oleuropein exhibited MIC values of 10 mg/ml for <i>C. albicans</i> and 5 mg/ml for <i>S. aureus</i>. It significantly (P< 0.05) reduced the adhesion of both microorganisms in a dose-dependent manner, with inhibition percentages of 78.43% and 75.91% for <i>C. albicans</i> and <i>S. aureus</i>, respectively. Additionally, oleuropein reduced the CSH of <i>C. albicans</i>, indicating its potential to interfere with adhesion mechanisms. In addition, oleuropein exhibited inhibition of GTF in <i>C. albicans.</i></p><p><strong>Conclusion: </strong>Oleuropein demonstrates significant antimicrobial and anti-adhesive properties against <i>C. albicans</i> and <i>S. aureus,</i> indicating its potential as a therapeutic agent for preventing biofilm-related infections. 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Impact of oleuropein on Candida albicans and Staphylococcus aureus adhesion and its mediated toxicity in Zebrafish (Danio rerio) embryos.
Background and objectives: The rising prevalence of antibiotic resistance and biofilm-associated infections poses significant challenges in clinical settings. This study investigates the antimicrobial and anti-adhesive properties of oleuropein, a compound derived from olive leaves, against Candida albicans and Staphylococcus aureus.
Materials and methods: This study was conducted on Candida albicans (fluconazole-resistant/susceptible) and Staphylococcus aureus (methicillin-resistant/susceptible). The antifungal, antibacterial, anti-adhesion, and cell surface hydrophobicity (CSH) effects of oleuropein were evaluated. The impact of oleuropein on germ tube formation (GTF) in C. albicans was assessed. Finally, the toxicity of oleuropein was evaluated in zebrafish embryos.
Results: Oleuropein exhibited MIC values of 10 mg/ml for C. albicans and 5 mg/ml for S. aureus. It significantly (P< 0.05) reduced the adhesion of both microorganisms in a dose-dependent manner, with inhibition percentages of 78.43% and 75.91% for C. albicans and S. aureus, respectively. Additionally, oleuropein reduced the CSH of C. albicans, indicating its potential to interfere with adhesion mechanisms. In addition, oleuropein exhibited inhibition of GTF in C. albicans.
Conclusion: Oleuropein demonstrates significant antimicrobial and anti-adhesive properties against C. albicans and S. aureus, indicating its potential as a therapeutic agent for preventing biofilm-related infections. However, careful dosage management is crucial due to its observed toxicity at higher concentrations.
期刊介绍：
The Iranian Journal of Microbiology (IJM) is an international, multi-disciplinary, peer-reviewed journal that provides rapid publication of the most advanced scientific research in the areas of basic and applied research on bacteria and other micro-organisms, including bacteria, viruses, yeasts, fungi, microalgae, and protozoa concerning the development of tools for diagnosis and disease control, epidemiology, antimicrobial agents, clinical microbiology, immunology, Genetics, Genomics and Molecular Biology. Contributions may be in the form of original research papers, review articles, short communications, case reports, technical reports, and letters to the Editor. Research findings must be novel and the original data must be available for review by the Editors, if necessary. Studies that are preliminary, of weak originality or merely descriptive as well as negative results are not appropriate for the journal. Papers considered for publication must be unpublished work (except in an abstract form) that is not under consideration for publication anywhere else, and all co-authors should have agreed to the submission. Manuscripts should be written in English.
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