{"title":"HPLC色谱柱相体积测定中的挑战。","authors":"Victor David, Jana Petre, Serban C Moldoveanu","doi":"10.3390/molecules30092062","DOIUrl":null,"url":null,"abstract":"<p><p>The evaluation of the time spent by a solute exclusively in the mobile phase (dead time) is of fundamental interest for the interpretation of the retention data and obtainment of thermodynamic parameters for the HPLC process. This parameter depends on the volume occupied by the mobile phase and on the volume of the effective stationary phase from the HPLC column, and the measurement of these volumes poses a real challenge. This review discusses the evaluation of volumes of various phases involved in the retention process of solutes, which are related to the dead time, and the phase ratio for the separation. This paper attempts to cover as many points of view as possible regarding this topic in liquid chromatography, which is of importance for almost all separation mechanisms.</p>","PeriodicalId":19041,"journal":{"name":"Molecules","volume":"30 9","pages":""},"PeriodicalIF":4.2000,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12073774/pdf/","citationCount":"0","resultStr":"{\"title\":\"Challenges in the Measurement of the Volume of Phases for HPLC Columns.\",\"authors\":\"Victor David, Jana Petre, Serban C Moldoveanu\",\"doi\":\"10.3390/molecules30092062\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The evaluation of the time spent by a solute exclusively in the mobile phase (dead time) is of fundamental interest for the interpretation of the retention data and obtainment of thermodynamic parameters for the HPLC process. This parameter depends on the volume occupied by the mobile phase and on the volume of the effective stationary phase from the HPLC column, and the measurement of these volumes poses a real challenge. This review discusses the evaluation of volumes of various phases involved in the retention process of solutes, which are related to the dead time, and the phase ratio for the separation. This paper attempts to cover as many points of view as possible regarding this topic in liquid chromatography, which is of importance for almost all separation mechanisms.</p>\",\"PeriodicalId\":19041,\"journal\":{\"name\":\"Molecules\",\"volume\":\"30 9\",\"pages\":\"\"},\"PeriodicalIF\":4.2000,\"publicationDate\":\"2025-05-06\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12073774/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Molecules\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://doi.org/10.3390/molecules30092062\",\"RegionNum\":2,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Molecules","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.3390/molecules30092062","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Challenges in the Measurement of the Volume of Phases for HPLC Columns.
The evaluation of the time spent by a solute exclusively in the mobile phase (dead time) is of fundamental interest for the interpretation of the retention data and obtainment of thermodynamic parameters for the HPLC process. This parameter depends on the volume occupied by the mobile phase and on the volume of the effective stationary phase from the HPLC column, and the measurement of these volumes poses a real challenge. This review discusses the evaluation of volumes of various phases involved in the retention process of solutes, which are related to the dead time, and the phase ratio for the separation. This paper attempts to cover as many points of view as possible regarding this topic in liquid chromatography, which is of importance for almost all separation mechanisms.
期刊介绍：
Molecules (ISSN 1420-3049, CODEN: MOLEFW) is an open access journal of synthetic organic chemistry and natural product chemistry. All articles are peer-reviewed and published continously upon acceptance. Molecules is published by MDPI, Basel, Switzerland. Our aim is to encourage chemists to publish as much as possible their experimental detail, particularly synthetic procedures and characterization information. There is no restriction on the length of the experimental section. In addition, availability of compound samples is published and considered as important information. Authors are encouraged to register or deposit their chemical samples through the non-profit international organization Molecular Diversity Preservation International (MDPI). Molecules has been launched in 1996 to preserve and exploit molecular diversity of both, chemical information and chemical substances.
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