{"title":"干燥综合征唇小唾液腺活检CD20的评价。","authors":"Ayesha Tabassum, Malathi Narasimhan, Thamizhchelvan Hari, Sharada T Rajan, Vijaya Nirmala Subramani","doi":"10.4103/jomfp.jomfp_547_23","DOIUrl":null,"url":null,"abstract":"<p><strong>Context: </strong>Sjogren's syndrome (SjS) is an auto-immune disease with a chronic inflammation causing dysfunction of salivary and lacrimal glands, leading to dry eyes and dry mouth. Focal lymphocytic sialadenitis (FLS) and focus score (FS) are significant diagnostic criteria in SjS.</p><p><strong>Aims: </strong>To evaluate the FLS and FS and quantify FS in H&E sections of normal salivary gland, inflammatory conditions, and SjS group.</p><p><strong>Settings and design: </strong>A total of 60 cases were included and divided equally among the groups - SjS, inflammatory conditions, and normal salivary gland.</p><p><strong>Methods and material: </strong>The clinical symptoms, associated disease, and serological tests (ANA, SSA, SSB, RF) were obtained from the records of the patient. Immunohistochemistry (IHC) staining was done (anti-CD20 monoclonal antibody). All the H&E and IHC slides were assessed by independent observers and were taken up for evaluation.</p><p><strong>Results and conclusions: </strong>In H&E sections, FLS and FS were found only in the SjS patients and not in normal and inflammatory conditions. CD20 expression was found to be positive in 55% of SjS cases but negative in the normal and inflammatory groups. FS had been upgraded in IHC CD20 staining. FLS and FSs should be strictly evaluated according to the criteria. It was found only in SjS patients and not in normal and inflammatory conditions. Furthermore, CD20 can be used as an adjunct marker to decide the theranostic and prognostic markers of SjS. The results showed that criteria for scoring FS should be strictly followed and CD20 IHC can be used as a prognostic marker.</p>","PeriodicalId":38846,"journal":{"name":"Journal of Oral and Maxillofacial Pathology","volume":"29 1","pages":"24-29"},"PeriodicalIF":0.0000,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12002588/pdf/","citationCount":"0","resultStr":"{\"title\":\"Evaluation of CD20 in labial minor salivary gland biopsies in Sjogren's syndrome.\",\"authors\":\"Ayesha Tabassum, Malathi Narasimhan, Thamizhchelvan Hari, Sharada T Rajan, Vijaya Nirmala Subramani\",\"doi\":\"10.4103/jomfp.jomfp_547_23\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Context: </strong>Sjogren's syndrome (SjS) is an auto-immune disease with a chronic inflammation causing dysfunction of salivary and lacrimal glands, leading to dry eyes and dry mouth. Focal lymphocytic sialadenitis (FLS) and focus score (FS) are significant diagnostic criteria in SjS.</p><p><strong>Aims: </strong>To evaluate the FLS and FS and quantify FS in H&E sections of normal salivary gland, inflammatory conditions, and SjS group.</p><p><strong>Settings and design: </strong>A total of 60 cases were included and divided equally among the groups - SjS, inflammatory conditions, and normal salivary gland.</p><p><strong>Methods and material: </strong>The clinical symptoms, associated disease, and serological tests (ANA, SSA, SSB, RF) were obtained from the records of the patient. Immunohistochemistry (IHC) staining was done (anti-CD20 monoclonal antibody). All the H&E and IHC slides were assessed by independent observers and were taken up for evaluation.</p><p><strong>Results and conclusions: </strong>In H&E sections, FLS and FS were found only in the SjS patients and not in normal and inflammatory conditions. CD20 expression was found to be positive in 55% of SjS cases but negative in the normal and inflammatory groups. FS had been upgraded in IHC CD20 staining. FLS and FSs should be strictly evaluated according to the criteria. It was found only in SjS patients and not in normal and inflammatory conditions. Furthermore, CD20 can be used as an adjunct marker to decide the theranostic and prognostic markers of SjS. The results showed that criteria for scoring FS should be strictly followed and CD20 IHC can be used as a prognostic marker.</p>\",\"PeriodicalId\":38846,\"journal\":{\"name\":\"Journal of Oral and Maxillofacial Pathology\",\"volume\":\"29 1\",\"pages\":\"24-29\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2025-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12002588/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Oral and Maxillofacial Pathology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.4103/jomfp.jomfp_547_23\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/3/28 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q3\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Oral and Maxillofacial Pathology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4103/jomfp.jomfp_547_23","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/3/28 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"Medicine","Score":null,"Total":0}
Evaluation of CD20 in labial minor salivary gland biopsies in Sjogren's syndrome.
Context: Sjogren's syndrome (SjS) is an auto-immune disease with a chronic inflammation causing dysfunction of salivary and lacrimal glands, leading to dry eyes and dry mouth. Focal lymphocytic sialadenitis (FLS) and focus score (FS) are significant diagnostic criteria in SjS.
Aims: To evaluate the FLS and FS and quantify FS in H&E sections of normal salivary gland, inflammatory conditions, and SjS group.
Settings and design: A total of 60 cases were included and divided equally among the groups - SjS, inflammatory conditions, and normal salivary gland.
Methods and material: The clinical symptoms, associated disease, and serological tests (ANA, SSA, SSB, RF) were obtained from the records of the patient. Immunohistochemistry (IHC) staining was done (anti-CD20 monoclonal antibody). All the H&E and IHC slides were assessed by independent observers and were taken up for evaluation.
Results and conclusions: In H&E sections, FLS and FS were found only in the SjS patients and not in normal and inflammatory conditions. CD20 expression was found to be positive in 55% of SjS cases but negative in the normal and inflammatory groups. FS had been upgraded in IHC CD20 staining. FLS and FSs should be strictly evaluated according to the criteria. It was found only in SjS patients and not in normal and inflammatory conditions. Furthermore, CD20 can be used as an adjunct marker to decide the theranostic and prognostic markers of SjS. The results showed that criteria for scoring FS should be strictly followed and CD20 IHC can be used as a prognostic marker.
期刊介绍：
The journal of Oral and Maxillofacial Pathology [ISSN:print-(0973-029X, online-1998-393X)] is a tri-annual journal published on behalf of “The Indian Association of Oral and Maxillofacial Pathologists” (IAOMP). The publication of JOMFP was started in the year 1993. The journal publishes papers on a wide spectrum of topics associated with the scope of Oral and Maxillofacial Pathology, also, ensuring scientific merit and quality. It is a comprehensive reading material for the professionals who want to upgrade their diagnostic skills in Oral Diseases; allows exposure to newer topics and methods of research in the Oral-facial Tissues and Pathology. New features allow an open minded thinking and approach to various pathologies. It also encourages authors to showcase quality work done by them and to compile relevant cases which are diagnostically challenging. The Journal takes pride in maintaining the quality of articles and photomicrographs.
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