A case for community metadata standards in cryo-electron tomography.

In the past decade, cryo-electron microscopy and single particle analysis (SPA) have quickly become key methods in structural biology. In particular, increased access to equipment and streamlined software has enabled new users to successfully carry out SPA projects. At the same time, cryo-electron tomography (cryo-ET) has also made great technical strides, most notably with cellular cryo-ET. While many challenges remain, developments in hardware and automation have made cellular cryo-ET specimen preparation and data collection more accessible than ever. There is also a growing field of cryo-ET software developers, but the wide variety of biological specimens and scientific goals that can be pursued using cryo-ET makes it difficult to develop processing workflows analogous to those in SPA; this becomes a major barrier to entry for new users. In this perspective, I make a case that the development of standardized metadata can play a key role in reducing such barriers and allow for an ecosystem that enables new users to enter the field while retaining a diversity of processing approaches.