Masoumeh Sadat Hosseini, Masoumeh Navidinia, Sima Sadat Seyedjavadi, Mehdi Goudarzi, Helia Rasouli, Amir Mohsen Mahdavian, Elina Rahimi Zamani
{"title":"铜绿假单胞菌毒素对伊朗临床分离白色念珠菌唑类抗真菌耐药性的拮抗作用评价。","authors":"Masoumeh Sadat Hosseini, Masoumeh Navidinia, Sima Sadat Seyedjavadi, Mehdi Goudarzi, Helia Rasouli, Amir Mohsen Mahdavian, Elina Rahimi Zamani","doi":"10.18502/ijm.v17i2.18395","DOIUrl":null,"url":null,"abstract":"<p><strong>Background and objectives: </strong>The azole antifungals are the most frequent class used to treat <i>Candida</i> infections. It is essential to elucidate the potential of natural compounds as an alternative in eliminating <i>Candida albicans (C. albicans).</i> Therefore, in the present study, the antagonistic effect of <i>Pseudomonas aeruginosa</i> toxins on azole antifungal resistance in <i>C. albicans</i> species was investigated.</p><p><strong>Materials and methods: </strong>In this study, 28 <i>C. albicans</i> species with azole antifungal resistance were obtained from patients at Shohadaye Tajrish Hospital. The effect of toxins, such as phenazine, pyocyanin, pyoverdine, and fluorescein, was examined on <i>C. albicans</i> species. The antifungal activity of these toxins against <i>C. albicans</i> spp. was determined using methods such as minimal inhibitory concentration (MIC <sub>90</sub>), radial diffusion assay (RDA), and detection of reactive oxygen species (ROS).</p><p><strong>Results: </strong>The prevalence of <i>C. albicans</i> strains in urinary catheters, surgical wounds, respiratory tracts, blood, and standard strains was 46.3%, 21.4%, 25%, 7.14%, and 3.57%, respectively. The MIC values were reported as 32 μg/ml for phenazine, and 128 μg/ml for pyoverdine, pyocyanin, and fluorescein. The results showed that phenazine exhibited higher inhibitory effects against <i>C. albicans</i> isolated from clinical samples compared to the other toxins. After exposure to phenazines (20 μg/ml), 65-70% of yeast cells of <i>C. albicans</i> spp. showed rhodamine 123 fluorescence, indicating high intracellular reactive oxygen species (ROS) production.</p><p><strong>Conclusion: </strong>The antifungal effect of different toxins in <i>C. albicans</i> spp. may be due to ROS-mediated apoptotic death. The results suggest that phenazine has high potential in controlling <i>C. albicans</i>. This natural compounds are a potential alternative for eliminating this yeast.</p>","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"17 2","pages":"293-302"},"PeriodicalIF":1.3000,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12053413/pdf/","citationCount":"0","resultStr":"{\"title\":\"Evaluation of the antagonistic effect of <i>Pseudomonas aeruginosa</i> toxins on azole antifungal resistance in <i>Candida albicans</i> species isolated from clinical samples in Iran.\",\"authors\":\"Masoumeh Sadat Hosseini, Masoumeh Navidinia, Sima Sadat Seyedjavadi, Mehdi Goudarzi, Helia Rasouli, Amir Mohsen Mahdavian, Elina Rahimi Zamani\",\"doi\":\"10.18502/ijm.v17i2.18395\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background and objectives: </strong>The azole antifungals are the most frequent class used to treat <i>Candida</i> infections. It is essential to elucidate the potential of natural compounds as an alternative in eliminating <i>Candida albicans (C. albicans).</i> Therefore, in the present study, the antagonistic effect of <i>Pseudomonas aeruginosa</i> toxins on azole antifungal resistance in <i>C. albicans</i> species was investigated.</p><p><strong>Materials and methods: </strong>In this study, 28 <i>C. albicans</i> species with azole antifungal resistance were obtained from patients at Shohadaye Tajrish Hospital. The effect of toxins, such as phenazine, pyocyanin, pyoverdine, and fluorescein, was examined on <i>C. albicans</i> species. The antifungal activity of these toxins against <i>C. albicans</i> spp. was determined using methods such as minimal inhibitory concentration (MIC <sub>90</sub>), radial diffusion assay (RDA), and detection of reactive oxygen species (ROS).</p><p><strong>Results: </strong>The prevalence of <i>C. albicans</i> strains in urinary catheters, surgical wounds, respiratory tracts, blood, and standard strains was 46.3%, 21.4%, 25%, 7.14%, and 3.57%, respectively. The MIC values were reported as 32 μg/ml for phenazine, and 128 μg/ml for pyoverdine, pyocyanin, and fluorescein. The results showed that phenazine exhibited higher inhibitory effects against <i>C. albicans</i> isolated from clinical samples compared to the other toxins. After exposure to phenazines (20 μg/ml), 65-70% of yeast cells of <i>C. albicans</i> spp. showed rhodamine 123 fluorescence, indicating high intracellular reactive oxygen species (ROS) production.</p><p><strong>Conclusion: </strong>The antifungal effect of different toxins in <i>C. albicans</i> spp. may be due to ROS-mediated apoptotic death. The results suggest that phenazine has high potential in controlling <i>C. albicans</i>. This natural compounds are a potential alternative for eliminating this yeast.</p>\",\"PeriodicalId\":14633,\"journal\":{\"name\":\"Iranian Journal of Microbiology\",\"volume\":\"17 2\",\"pages\":\"293-302\"},\"PeriodicalIF\":1.3000,\"publicationDate\":\"2025-04-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12053413/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Iranian Journal of Microbiology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.18502/ijm.v17i2.18395\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Iranian Journal of Microbiology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.18502/ijm.v17i2.18395","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
Evaluation of the antagonistic effect of Pseudomonas aeruginosa toxins on azole antifungal resistance in Candida albicans species isolated from clinical samples in Iran.
Background and objectives: The azole antifungals are the most frequent class used to treat Candida infections. It is essential to elucidate the potential of natural compounds as an alternative in eliminating Candida albicans (C. albicans). Therefore, in the present study, the antagonistic effect of Pseudomonas aeruginosa toxins on azole antifungal resistance in C. albicans species was investigated.
Materials and methods: In this study, 28 C. albicans species with azole antifungal resistance were obtained from patients at Shohadaye Tajrish Hospital. The effect of toxins, such as phenazine, pyocyanin, pyoverdine, and fluorescein, was examined on C. albicans species. The antifungal activity of these toxins against C. albicans spp. was determined using methods such as minimal inhibitory concentration (MIC 90), radial diffusion assay (RDA), and detection of reactive oxygen species (ROS).
Results: The prevalence of C. albicans strains in urinary catheters, surgical wounds, respiratory tracts, blood, and standard strains was 46.3%, 21.4%, 25%, 7.14%, and 3.57%, respectively. The MIC values were reported as 32 μg/ml for phenazine, and 128 μg/ml for pyoverdine, pyocyanin, and fluorescein. The results showed that phenazine exhibited higher inhibitory effects against C. albicans isolated from clinical samples compared to the other toxins. After exposure to phenazines (20 μg/ml), 65-70% of yeast cells of C. albicans spp. showed rhodamine 123 fluorescence, indicating high intracellular reactive oxygen species (ROS) production.
Conclusion: The antifungal effect of different toxins in C. albicans spp. may be due to ROS-mediated apoptotic death. The results suggest that phenazine has high potential in controlling C. albicans. This natural compounds are a potential alternative for eliminating this yeast.
期刊介绍：
The Iranian Journal of Microbiology (IJM) is an international, multi-disciplinary, peer-reviewed journal that provides rapid publication of the most advanced scientific research in the areas of basic and applied research on bacteria and other micro-organisms, including bacteria, viruses, yeasts, fungi, microalgae, and protozoa concerning the development of tools for diagnosis and disease control, epidemiology, antimicrobial agents, clinical microbiology, immunology, Genetics, Genomics and Molecular Biology. Contributions may be in the form of original research papers, review articles, short communications, case reports, technical reports, and letters to the Editor. Research findings must be novel and the original data must be available for review by the Editors, if necessary. Studies that are preliminary, of weak originality or merely descriptive as well as negative results are not appropriate for the journal. Papers considered for publication must be unpublished work (except in an abstract form) that is not under consideration for publication anywhere else, and all co-authors should have agreed to the submission. Manuscripts should be written in English.
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