在离靶不同距离的发光二极管照射下成骨潜能的比较评价:一项体外研究。

IF 1 Q3 DENTISTRY, ORAL SURGERY & MEDICINE
Divyabharathi Selvam, Venkat Rengasamy
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引用次数: 0

摘要

目的:评价和比较不同距离LED照射对MG-63成骨样细胞在钛盘上的成骨作用。环境和设计:这项体外实验研究涉及在钛盘上培养的人骨肉瘤(MG-63)细胞,并在不同距离上进行LED照射,与未照射的对照组相比。材料与方法:将42个钛片分为3组:对照组(无照射)、LED组1(距离10 mm)和LED组2(距离20 mm)。将MG-63细胞接种到所有的圆盘上并进行预培养。实验组接受660nm红色LED光照射2分钟,对照组不接受照射。在24和48小时用MTT法测定细胞活力。分别在72、96和120小时评估细胞附着、生长和增殖情况。统计学分析使用:数据分析使用SPSS v28.0。采用双因素方差分析和Tukey事后检验来确定各组间不同时间点的统计学差异。p值< 0.05被认为是显著的。结果:两组小鼠成骨活性均显著高于对照组(p < 0.05)。第1组(10 mm)细胞活力、附着和增殖最高。第2组(20 mm)有中度改善,但不如第1组,提示距离依赖效应。结论:LED治疗促进钛表面成骨,照射距离为10 mm时效果最佳。这些发现支持使用优化的LED治疗来改善骨愈合和种植体整合。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Comparative evaluation of osteogenic potential of light-emitting diode irradiation at varying distances from the target: An in vitro study.

Aim: To evaluate and compare the osteogenic effects of LED irradiation at varying distances using MG-63 osteoblast-like cells cultured on titanium discs.

Settings and design: This in vitro experimental study involved human osteosarcoma (MG-63) cells cultured on titanium discs and subjected to LED irradiation at different distances, compared to a non-irradiated control group.

Materials and methods: Forty-two titanium discs were divided into three groups: Control (no irradiation), LED Group 1 (10 mm distance), and LED Group 2 (20 mm distance). All discs were seeded with MG-63 cells and pre-cultured. Experimental groups received a single 2-minute exposure to 660 nm red LED light, while controls received no exposure. Cell viability was measured at 24 and 48 hours using the MTT assay. Cell attachment, growth, and proliferation were assessed at 72, 96, and 120 hours.

Statistical analysis used: Data were analyzed using SPSS v28.0. Two-way ANOVA followed by Tukey's post-hoc test was applied to identify statistically significant differences among groups across time points. A p-value < 0.05 was considered significant.

Results: Both LED-irradiated groups showed significantly enhanced osteogenic activity compared to controls (p < 0.05). Group 1 (10 mm) exhibited the highest cell viability, attachment, and proliferation. Group 2 (20 mm) showed moderate improvement but was inferior to Group 1, indicating distance-dependent effects.

Conclusion: LED therapy enhances osteogenesis on titanium surfaces, with the greatest efficacy observed at a 10 mm irradiation distance. These findings support the use of optimized LED therapy to improve bone healing and implant integration.

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来源期刊
The Journal of Indian Prosthodontic Society
The Journal of Indian Prosthodontic Society DENTISTRY, ORAL SURGERY & MEDICINE-
CiteScore
2.20
自引率
8.30%
发文量
26
审稿时长
20 weeks
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