Isah Musa Maishanu, Adeshina O Gbonjubola, Hussaini Mujahid, Busayo O Olayinka
{"title":"尼日利亚西北部Birnin Kebbi联邦医学中心金黄色葡萄球菌临床分离株酚溶性调素-mec的分子检测","authors":"Isah Musa Maishanu, Adeshina O Gbonjubola, Hussaini Mujahid, Busayo O Olayinka","doi":"10.3205/dgkh000538","DOIUrl":null,"url":null,"abstract":"<p><strong>Aim: </strong>This study was carried out to isolate and detect virulence genes associated with <i>Staphylococcus (S.) aureus</i> clinical isolates from the Federal Medical Center Birnin Kebbi, Nigeria.</p><p><strong>Methods: </strong>To obtain <i>S. aureus</i> isolates, samples were taken from urine, sputum, blood and wound sources. <i>S. aureus</i> was phenotypically identified using Microgen staph ID system and PSM-mec and PVL genes were detected using polymerase chain reaction (PCR).</p><p><strong>Results: </strong>A total of 48 non-duplicate <i>S. aureus</i> isolates were obtained (21 from wound swabs, 7 from blood, 15 from urine, and 5 from sputum). From the 14 <i>S. aureus</i> isolates examined by PCR, the most abundant gene was <i>PSM-mec</i> (42.8%), while the PVL was the least abundant with 21.4%.</p><p><strong>Conclusion: </strong>Because it gives highly specific and accurate results, it is essential to use the PCR technique to detect <i>S. aureus</i> virulence determinants as well as PSM-mec and PVL as targets for antimicrobial agents.</p>","PeriodicalId":12738,"journal":{"name":"GMS Hygiene and Infection Control","volume":"20 ","pages":"Doc09"},"PeriodicalIF":1.6000,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12059804/pdf/","citationCount":"0","resultStr":"{\"title\":\"Molecular detection of phenol-soluble modulin-mec (PSM-mec) in Staphylococcus aureus clinical isolates from Federal Medical Center Birnin Kebbi, North-West, Nigeria.\",\"authors\":\"Isah Musa Maishanu, Adeshina O Gbonjubola, Hussaini Mujahid, Busayo O Olayinka\",\"doi\":\"10.3205/dgkh000538\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Aim: </strong>This study was carried out to isolate and detect virulence genes associated with <i>Staphylococcus (S.) aureus</i> clinical isolates from the Federal Medical Center Birnin Kebbi, Nigeria.</p><p><strong>Methods: </strong>To obtain <i>S. aureus</i> isolates, samples were taken from urine, sputum, blood and wound sources. <i>S. aureus</i> was phenotypically identified using Microgen staph ID system and PSM-mec and PVL genes were detected using polymerase chain reaction (PCR).</p><p><strong>Results: </strong>A total of 48 non-duplicate <i>S. aureus</i> isolates were obtained (21 from wound swabs, 7 from blood, 15 from urine, and 5 from sputum). From the 14 <i>S. aureus</i> isolates examined by PCR, the most abundant gene was <i>PSM-mec</i> (42.8%), while the PVL was the least abundant with 21.4%.</p><p><strong>Conclusion: </strong>Because it gives highly specific and accurate results, it is essential to use the PCR technique to detect <i>S. aureus</i> virulence determinants as well as PSM-mec and PVL as targets for antimicrobial agents.</p>\",\"PeriodicalId\":12738,\"journal\":{\"name\":\"GMS Hygiene and Infection Control\",\"volume\":\"20 \",\"pages\":\"Doc09\"},\"PeriodicalIF\":1.6000,\"publicationDate\":\"2025-03-06\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12059804/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"GMS Hygiene and Infection Control\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.3205/dgkh000538\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q3\",\"JCRName\":\"PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"GMS Hygiene and Infection Control","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.3205/dgkh000538","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q3","JCRName":"PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH","Score":null,"Total":0}
Molecular detection of phenol-soluble modulin-mec (PSM-mec) in Staphylococcus aureus clinical isolates from Federal Medical Center Birnin Kebbi, North-West, Nigeria.
Aim: This study was carried out to isolate and detect virulence genes associated with Staphylococcus (S.) aureus clinical isolates from the Federal Medical Center Birnin Kebbi, Nigeria.
Methods: To obtain S. aureus isolates, samples were taken from urine, sputum, blood and wound sources. S. aureus was phenotypically identified using Microgen staph ID system and PSM-mec and PVL genes were detected using polymerase chain reaction (PCR).
Results: A total of 48 non-duplicate S. aureus isolates were obtained (21 from wound swabs, 7 from blood, 15 from urine, and 5 from sputum). From the 14 S. aureus isolates examined by PCR, the most abundant gene was PSM-mec (42.8%), while the PVL was the least abundant with 21.4%.
Conclusion: Because it gives highly specific and accurate results, it is essential to use the PCR technique to detect S. aureus virulence determinants as well as PSM-mec and PVL as targets for antimicrobial agents.
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