胃肠道疾病患者血清唾液幽门螺杆菌免疫球蛋白和寄生虫感染检测与个人卫生的相关性

IF 4.3 3区医学 Q1 GASTROENTEROLOGY & HEPATOLOGY

Gut Pathogens Pub Date : 2025-04-10 DOI:10.1186/s13099-025-00688-2

Faika Hassanein, Mohamed S Abdel-Latif, Amany I Shehata

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引用次数: 0

摘要

背景：由于几个危险因素，包括个人卫生不良和社会经济生活方式，胃肠道微生物感染在医疗保健个体（hci）中很常见。目的：这是第一个对hci进行分层的横断面研究，将个人卫生和社会经济生活方式与胃肠道微生物感染联系起来。此外，它比较了血清和唾液中幽门螺杆菌igg和IgA的水平，以评估唾液作为血清的非侵入性替代品的潜力。方法：根据Fisher公式，纳入200例患有胃炎的hci患者，包括医院工作人员、员工、护生、护士和医生。采集血液、唾液和粪便样本进行微生物感染调查。根据世卫组织指南对个人卫生和社会经济因素进行评分。显微镜下鉴定寄生虫感染，ELISA检测幽门螺杆菌抗原和抗体，ROC曲线分析确定诊断意义。结果：hci患者肠道微生物感染发生率较高。最常见的病原菌为囊虫（72%），其次为隐孢子虫（59.5%）。发现了单、双、多重感染病例。36例（18%）病例中存在幽门螺杆菌抗原，通常与肠道寄生虫共同感染。工人和护士感染率最高（100%），其次是员工（97.4%）和护生（81.7%），医生感染率最低（50%）。不良的个人卫生和社会经济生活方式与感染风险增加直接相关。血清和唾液中幽门螺杆菌igg阳性14例，阴性186例；幽门螺杆菌iga阳性2例，阴性198例。这些发现表明血清和唾液中幽门螺杆菌免疫球蛋白水平的一致性。结论：不良的个人卫生和社会经济生活方式显著增加了hci患者胃肠道微生物感染的风险。唾液免疫球蛋白显示与血清水平一致，表明唾液是检测幽门螺杆菌感染的可行的非侵入性替代方法。

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Sero-salivary detection of H. pylori immunoglobulins and parasitic infection among healthcare individuals suffering from gastrointestinal disorders with correlation to personal hygiene.

Background: Gastrointestinal microbial infections among healthcare individuals (HCIs) are common due to several risk factors, including poor personal hygiene and socio-economic lifestyle.

Objectives: This is the first cross-sectional study that stratifies HCIs to correlate personal hygiene and socio-economic lifestyle with gastrointestinal microbial infections. Additionally, it compares serum and saliva levels of H. pylori-IgG and IgA to assess the potential of saliva as a non-invasive alternative to serum.

Methods: Based on Fisher's formula, 200 HCIs suffering from gastritis-including hospital workers, employees, nursing students, nurses, and doctors-were enrolled. Blood, saliva, and stool samples were collected for microbial infection investigations. Personal hygiene and socio-economic factors were scored based on WHO guidelines. Parasitic infections were identified microscopically, while H. pylori antigen and antibodies were detected via ELISA, with diagnostic significance determined by ROC curve analysis.

Results: A high prevalence of intestinal microbial infections was observed among HCIs. Blastocystis spp. was the most common pathogen (72%), followed by Cryptosporidium spp. (59.5%). Cases of single, double, and multiple infections were detected. H. pylori antigen was present in 36 (18%) cases, often as a co-infection with intestinal parasites. Infection rates were highest among workers and nurses (100%), followed by employees (97.4%) and nursing students (81.7%), with doctors having the lowest rate (50%). Poor personal hygiene and socio-economic lifestyle were directly linked to increased infection risk. Additionally, H. pylori-IgG was positive in 14 cases and negative in 186 cases, while H. pylori-IgA was positive in 2 cases and negative in 198 cases in both serum and saliva. These findings indicate consistency between serum and saliva levels of H. pylori immunoglobulins.

Conclusions: Poor personal hygiene and socio-economic lifestyle significantly increase the risk of gastrointestinal microbial infections among HCIs. Salivary immunoglobulins show consistency with serum levels, suggesting saliva as a viable non-invasive alternative for detecting H. pylori infection.

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来源期刊

Gut Pathogens GASTROENTEROLOGY & HEPATOLOGY-MICROBIOLOGY

CiteScore

7.70

自引率

2.40%

发文量

期刊介绍： Gut Pathogens is a fast publishing, inclusive and prominent international journal which recognizes the need for a publishing platform uniquely tailored to reflect the full breadth of research in the biology and medicine of pathogens, commensals and functional microbiota of the gut. The journal publishes basic, clinical and cutting-edge research on all aspects of the above mentioned organisms including probiotic bacteria and yeasts and their products. The scope also covers the related ecology, molecular genetics, physiology and epidemiology of these microbes. The journal actively invites timely reports on the novel aspects of genomics, metagenomics, microbiota profiling and systems biology. Gut Pathogens will also consider, at the discretion of the editors, descriptive studies identifying a new genome sequence of a gut microbe or a series of related microbes (such as those obtained from new hosts, niches, settings, outbreaks and epidemics) and those obtained from single or multiple hosts at one or different time points (chronological evolution).