Ayten Ibrahimova, Ayşe Nur Önem, Mehmet Altun, Emin Ahmet Yeşil, Aslı Baysal, Hasan Saygin, Ahu Soyocak, Mustafa Özyürek
{"title":"一种新型次氯酸关闭型近红外荧光探针及其应用。","authors":"Ayten Ibrahimova, Ayşe Nur Önem, Mehmet Altun, Emin Ahmet Yeşil, Aslı Baysal, Hasan Saygin, Ahu Soyocak, Mustafa Özyürek","doi":"10.55730/1300-0527.3725","DOIUrl":null,"url":null,"abstract":"<p><p>Hypochlorous acid (HOCl) is a potent nonradical oxidant involved in various physiological processes, particularly within the human immune system. In this study, we introduce a novel, rapid, and highly efficient fluorometric method for the detection of HOCl. The method utilizes a near-infrared (NIR)-based fluorescent probe, NIR-QBH, which is characterized by its high sensitivity and chemical stability. NIR-QBH, containing olefinic C=C bonds, exhibits strong NIR emission at 660 nm (λ<sub>ex</sub> = 618 nm). The detection mechanism relies on the oxidation of the C=C bond in the NIR-QBH structure by HOCl, resulting in the formation of non-fluorescent products. With a detection limit of 0.23 μM, the probe demonstrates a fast response time of 4 min. Glutathione (GSH), an essential biothiol, was employed as a reference HOCl scavenger, and its HOCl scavenging activity was evaluated with an IC<sub>50</sub> value of 8.97 μM. Furthermore, the developed fluorometric assay was successfully applied for the detection of HOCl in fetal bovine serum (FBS) and aqueous solutions.</p>","PeriodicalId":23367,"journal":{"name":"Turkish Journal of Chemistry","volume":"49 2","pages":"241-253"},"PeriodicalIF":1.3000,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12068673/pdf/","citationCount":"0","resultStr":"{\"title\":\"A new turn off fluorescent NIR probe for hypochlorous acid and its applications.\",\"authors\":\"Ayten Ibrahimova, Ayşe Nur Önem, Mehmet Altun, Emin Ahmet Yeşil, Aslı Baysal, Hasan Saygin, Ahu Soyocak, Mustafa Özyürek\",\"doi\":\"10.55730/1300-0527.3725\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Hypochlorous acid (HOCl) is a potent nonradical oxidant involved in various physiological processes, particularly within the human immune system. In this study, we introduce a novel, rapid, and highly efficient fluorometric method for the detection of HOCl. The method utilizes a near-infrared (NIR)-based fluorescent probe, NIR-QBH, which is characterized by its high sensitivity and chemical stability. NIR-QBH, containing olefinic C=C bonds, exhibits strong NIR emission at 660 nm (λ<sub>ex</sub> = 618 nm). The detection mechanism relies on the oxidation of the C=C bond in the NIR-QBH structure by HOCl, resulting in the formation of non-fluorescent products. With a detection limit of 0.23 μM, the probe demonstrates a fast response time of 4 min. Glutathione (GSH), an essential biothiol, was employed as a reference HOCl scavenger, and its HOCl scavenging activity was evaluated with an IC<sub>50</sub> value of 8.97 μM. Furthermore, the developed fluorometric assay was successfully applied for the detection of HOCl in fetal bovine serum (FBS) and aqueous solutions.</p>\",\"PeriodicalId\":23367,\"journal\":{\"name\":\"Turkish Journal of Chemistry\",\"volume\":\"49 2\",\"pages\":\"241-253\"},\"PeriodicalIF\":1.3000,\"publicationDate\":\"2025-03-29\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12068673/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Turkish Journal of Chemistry\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://doi.org/10.55730/1300-0527.3725\",\"RegionNum\":4,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q3\",\"JCRName\":\"CHEMISTRY, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Turkish Journal of Chemistry","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.55730/1300-0527.3725","RegionNum":4,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q3","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
A new turn off fluorescent NIR probe for hypochlorous acid and its applications.
Hypochlorous acid (HOCl) is a potent nonradical oxidant involved in various physiological processes, particularly within the human immune system. In this study, we introduce a novel, rapid, and highly efficient fluorometric method for the detection of HOCl. The method utilizes a near-infrared (NIR)-based fluorescent probe, NIR-QBH, which is characterized by its high sensitivity and chemical stability. NIR-QBH, containing olefinic C=C bonds, exhibits strong NIR emission at 660 nm (λex = 618 nm). The detection mechanism relies on the oxidation of the C=C bond in the NIR-QBH structure by HOCl, resulting in the formation of non-fluorescent products. With a detection limit of 0.23 μM, the probe demonstrates a fast response time of 4 min. Glutathione (GSH), an essential biothiol, was employed as a reference HOCl scavenger, and its HOCl scavenging activity was evaluated with an IC50 value of 8.97 μM. Furthermore, the developed fluorometric assay was successfully applied for the detection of HOCl in fetal bovine serum (FBS) and aqueous solutions.
期刊介绍：
The Turkish Journal of Chemistry is a bimonthly multidisciplinary journal published by the Scientific and Technological Research Council of Turkey (TÜBİTAK).
The journal is dedicated to dissemination of knowledge in all disciplines of chemistry (organic, inorganic, physical, polymeric, technical, theoretical and analytical chemistry) as well as research at the interface with other sciences especially in chemical engineering where molecular aspects are key to the findings.
The journal accepts English-language original manuscripts and contribution is open to researchers of all nationalities.
The journal publishes refereed original papers, reviews, letters to editor and issues devoted to special fields.
All manuscripts are peer-reviewed and electronic processing ensures accurate reproduction of text and data, plus publication times as short as possible.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
