爱德华氏菌III型和VI型分泌系统突变株作为减毒活疫苗候选株。

IF 1.7 4区 农林科学 Q3 FISHERIES
Matthew L Rogge, Ahmad A Elkamel, Ronald L Thune
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引用次数: 0

摘要

目的:致鲶鱼肠性败血症的病原体爱德华氏菌(Edwardsiella ictaluri)给美国海峡鲶鱼(Channel catfish Ictalurus punctatus)养殖者造成了巨大的经济损失。本研究评估了三种伊克塔卢杆菌菌株,每一株都携带III型或VI型分泌系统基因的无标记缺失突变,作为海峡鲶鱼肠败血症减毒活疫苗的候选株。评估了每种突变体在通道鲶鱼细胞中的复制、体内入侵和持久性、毒力以及对野生型伊卡塔uri菌株提供单剂量保护的能力。方法:构建esrC、eseG和evpC基因缺失的3个等基因突变体。EsrC是一种T3SS编码的蛋白,可调控多种毒力基因的表达,包括T3SS效应蛋白EseG和T6SS结构蛋白EvpC。评估了每个突变株在通道鲶鱼卵巢细胞和头肾源性巨噬细胞中的复制能力。对通道鲶鱼也进行了突变菌株挑战,以评估突变是否影响了头肾内的入侵、定植或持久性,是否降低了通道鲶鱼的死亡率,或在随后的野生型E. ictaluri暴露后诱导对疾病的保护。结果:每个突变体都保持了在头肾源性巨噬细胞和通道鲶鱼卵巢细胞内复制的能力,以及入侵和定植头肾的能力。虽然能够在细胞内复制并成功定植鲶鱼组织,但这三种突变体在组织中持续存在并导致死亡的能力都显著减弱。在暴露于野生型伊氏伊氏杆菌之前,将突变菌株浸泡28 d,其死亡率显著低于浸泡在无菌肉汤中的鱼,相对存活率在95%至100%之间。结论:研究结果表明,通过靶向突变一个或多个伊克塔卢利杆菌T3SS和T6SS基因,有可能开发出伊克塔卢利杆菌减毒活疫苗株。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Edwardsiella ictaluri type III and type VI secretion system mutant strains as candidates for live attenuated vaccines.

Objective: Edwardsiella ictaluri, the causative agent of enteric septicemia of catfish, causes substantial economic losses to Channel Catfish Ictalurus punctatus producers in the United States. This study evaluates three E. ictaluri strains, each carrying a markerless deletion mutation in a type III or type VI secretion system gene, as candidates for a live attenuated vaccine against enteric septicemia of catfish in Channel Catfish. Replication in Channel Catfish cells, in vivo invasion and persistence, virulence, and the ability to provide single-dose protection against a wild-type E. ictaluri strain were evaluated for each mutant.

Methods: In this study, three isogenic mutants were constructed that introduced deletions in esrC, eseG, and evpC. EsrC is a T3SS-encoded protein that regulates expression of multiple virulence genes, including the T3SS effector EseG and the T6SS structural protein EvpC. Each mutant strain was evaluated for its ability to replicate in Channel Catfish ovary cells and head-kidney-derived macrophage cells. Channel Catfish were also challenged with the mutant strains to evaluate if mutation affected invasion, colonization, or persistence within the head kidney, attenuated mortalities in Channel Catfish, or induced protection against disease following subsequent wild-type E. ictaluri exposure.

Results: Each mutant maintained the ability to replicate within head-kidney-derived macrophage cells and Channel Catfish ovary cells, as well as invade and colonize the head kidney. Although able to replicate intracellularly and successfully colonize catfish tissue, all three mutants were significantly attenuated in their ability to persist in tissues and cause mortality. A single immersion in mutant strains 28 d prior to exposure to wild-type E. ictaluri resulted in significantly lower mortality than fish immersed in sterile broth, with relative percent survival ranging from 95% to 100%.

Conclusions: The findings demonstrate the potential for the development of live attenuated E. ictaluri vaccine strains through the targeted mutation of one or more E. ictaluri T3SS and T6SS genes.

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来源期刊
Journal of aquatic animal health
Journal of aquatic animal health 农林科学-兽医学
CiteScore
2.50
自引率
0.00%
发文量
25
审稿时长
>24 weeks
期刊介绍: The Journal of Aquatic Animal Health serves the international community of scientists and culturists concerned with the health of aquatic organisms. It carries research papers on the causes, effects, treatments, and prevention of diseases of marine and freshwater organisms, particularly fish and shellfish. In addition, it contains papers that describe biochemical and physiological investigations into fish health that relate to assessing the impacts of both environmental and pathogenic features.
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