{"title":"多杀性巴氏杆菌B帽的毒力基因及其对小鼠的潜在交叉保护作用。","authors":"Adam Bashir Tawor, Osman Erganiş, Aslı Balevi","doi":"10.1007/s10123-025-00658-3","DOIUrl":null,"url":null,"abstract":"<p><p>Pasteurella multocida is a Gram-negative coccobacillus from the Pasteurellaceae family, commonly residing as a commensal organism in the respiratory tracts of healthy animals. However, it possesses multiple virulence factors and can cause severe respiratory diseases. This study aimed to characterize P. multocida and its virulence genes and evaluate the immunogenicity of an inactivated vaccine of serogroup B using different administration routes. A total of 250 samples were collected from animals showing respiratory symptoms. Using 5% blood agar, 27 P. multocida isolates were obtained, and 21 (8.4%) were confirmed via PCR targeting the kmt1 gene. Nineteen virulence-associated genes were screened, categorized into outer membrane, fimbrial, somatic antigen, and iron-binding genes. The plpB, tadD, gatG, and hgbA genes were detected in both serogroup B and E isolates, whereas ompA, toxA, pcgD, latB, nctB, ppgB, natG, hgbB, and exbB were absent in all isolates. The immunogenicity of an inactivated P. multocida vaccine was evaluated in mice using subcutaneous and intramuscular routes. Subcutaneous vaccination produced a significantly higher antibody titer at 3 and 5 weeks post-vaccination with a 0.5 mL dose; in contrast, intramuscular immunization resulted in a rapid increase after booster doses. Analysis of variance (ANOVA) and Duncan's multiple range test (p < 0.05) revealed statistically significant differences between treatments. The comparison between subcutaneous and intramuscular routes also showed a significant difference (p < 0.001). This study concludes that although P. multocida serogroup B harbors fewer virulence factors, it effectively induces an immune response in mice but fails to provide cross-protection against the local serogroup E strain.</p>","PeriodicalId":14318,"journal":{"name":"International Microbiology","volume":" ","pages":"1895-1903"},"PeriodicalIF":2.3000,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Virulence genes of Pasteurella multocida cap B and its potential cross protection in mice.\",\"authors\":\"Adam Bashir Tawor, Osman Erganiş, Aslı Balevi\",\"doi\":\"10.1007/s10123-025-00658-3\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Pasteurella multocida is a Gram-negative coccobacillus from the Pasteurellaceae family, commonly residing as a commensal organism in the respiratory tracts of healthy animals. However, it possesses multiple virulence factors and can cause severe respiratory diseases. This study aimed to characterize P. multocida and its virulence genes and evaluate the immunogenicity of an inactivated vaccine of serogroup B using different administration routes. A total of 250 samples were collected from animals showing respiratory symptoms. Using 5% blood agar, 27 P. multocida isolates were obtained, and 21 (8.4%) were confirmed via PCR targeting the kmt1 gene. Nineteen virulence-associated genes were screened, categorized into outer membrane, fimbrial, somatic antigen, and iron-binding genes. The plpB, tadD, gatG, and hgbA genes were detected in both serogroup B and E isolates, whereas ompA, toxA, pcgD, latB, nctB, ppgB, natG, hgbB, and exbB were absent in all isolates. The immunogenicity of an inactivated P. multocida vaccine was evaluated in mice using subcutaneous and intramuscular routes. Subcutaneous vaccination produced a significantly higher antibody titer at 3 and 5 weeks post-vaccination with a 0.5 mL dose; in contrast, intramuscular immunization resulted in a rapid increase after booster doses. Analysis of variance (ANOVA) and Duncan's multiple range test (p < 0.05) revealed statistically significant differences between treatments. The comparison between subcutaneous and intramuscular routes also showed a significant difference (p < 0.001). This study concludes that although P. multocida serogroup B harbors fewer virulence factors, it effectively induces an immune response in mice but fails to provide cross-protection against the local serogroup E strain.</p>\",\"PeriodicalId\":14318,\"journal\":{\"name\":\"International Microbiology\",\"volume\":\" \",\"pages\":\"1895-1903\"},\"PeriodicalIF\":2.3000,\"publicationDate\":\"2025-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International Microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1007/s10123-025-00658-3\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/5/1 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q3\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s10123-025-00658-3","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/5/1 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Virulence genes of Pasteurella multocida cap B and its potential cross protection in mice.
Pasteurella multocida is a Gram-negative coccobacillus from the Pasteurellaceae family, commonly residing as a commensal organism in the respiratory tracts of healthy animals. However, it possesses multiple virulence factors and can cause severe respiratory diseases. This study aimed to characterize P. multocida and its virulence genes and evaluate the immunogenicity of an inactivated vaccine of serogroup B using different administration routes. A total of 250 samples were collected from animals showing respiratory symptoms. Using 5% blood agar, 27 P. multocida isolates were obtained, and 21 (8.4%) were confirmed via PCR targeting the kmt1 gene. Nineteen virulence-associated genes were screened, categorized into outer membrane, fimbrial, somatic antigen, and iron-binding genes. The plpB, tadD, gatG, and hgbA genes were detected in both serogroup B and E isolates, whereas ompA, toxA, pcgD, latB, nctB, ppgB, natG, hgbB, and exbB were absent in all isolates. The immunogenicity of an inactivated P. multocida vaccine was evaluated in mice using subcutaneous and intramuscular routes. Subcutaneous vaccination produced a significantly higher antibody titer at 3 and 5 weeks post-vaccination with a 0.5 mL dose; in contrast, intramuscular immunization resulted in a rapid increase after booster doses. Analysis of variance (ANOVA) and Duncan's multiple range test (p < 0.05) revealed statistically significant differences between treatments. The comparison between subcutaneous and intramuscular routes also showed a significant difference (p < 0.001). This study concludes that although P. multocida serogroup B harbors fewer virulence factors, it effectively induces an immune response in mice but fails to provide cross-protection against the local serogroup E strain.
期刊介绍:
International Microbiology publishes information on basic and applied microbiology for a worldwide readership. The journal publishes articles and short reviews based on original research, articles about microbiologists and their work and questions related to the history and sociology of this science. Also offered are perspectives, opinion, book reviews and editorials.
A distinguishing feature of International Microbiology is its broadening of the term microbiology to include eukaryotic microorganisms.