{"title":"伊朗西北部Meshkin-Shahr地区孕妇刚地弓形虫血清学评价及分子基因分型","authors":"Shabnam Asfaram, Zabih Zarei, Roghayeh Teimoorpour, Zahra Heidari, Sohrab Iranpour, Payman Azghani, Seyed Eghbal Motavallibashi, Zahra Rakhshidan, Zahra Khademi, Behnam Mohammadi-Ghalehbin, Shahram Habibzadeh, Soheila Molaei","doi":"10.18502/ijpa.v20i1.18104","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>We aimed to investigate the serological and molecular characteristics of <i>Toxoplasma gondii</i> infection among pregnant women and their aborted placentas in Meshkin-Shahr City during 2019-2020.</p><p><strong>Methods: </strong>Blood samples of 210 pregnant women were evaluated for anti-<i>T. gondii</i> antibodies and related risk factors were determined. Also, the sera of aborted women and their buffy coats and aborted placenta tissues were used to detect anti-<i>Toxoplasma</i> antibodies and the parasite's DNA, respectively. The parasite genotypes were determined by the PCR-RFLP using the <i>SAG3</i> gene.</p><p><strong>Results: </strong>The overall prevalence of anti-<i>Toxoplasma</i> IgG was 24.3% and only 1% of participants were seropositive for the IgM antibody. There was a significant relationship between raw or unwashed vegetable consumption, contact with soil, vegetable/fruit washing type, and seropositivity (<i>P</i><0.05). During pregnancy, 4.7% of women encountered an abortion and 30% and 50% of cases were positive for IgG antibodies before and after abortion, respectively. Only two cases were IgM seropositive after abortion. In the avidity IgG test, 20% of cases showed low avidity. BLAST and phylogenetic analysis exhibited that all isolates belonged to the type III <i>T. gondii</i> genotype. Although two women with spontaneous abortions showed seropositivity for IgM <i>T. gondii</i> antibody, parasite DNA was detected in three cases.</p><p><strong>Conclusion: </strong>The seroprevalence of <i>Toxoplasma</i> infection is not high in pregnant women. Seropositive women are not safe from congenital transmission. <i>T. gondii</i> type III is the etiology of fetus infection in mothers with spontaneous abortion. It seems that screening and essential care are still necessary during pregnancy.</p>","PeriodicalId":14669,"journal":{"name":"Iranian Journal of Parasitology","volume":"20 1","pages":"44-53"},"PeriodicalIF":0.9000,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11978203/pdf/","citationCount":"0","resultStr":"{\"title\":\"Serological Evaluation and Molecular Genotyping of <i>Toxoplasma gondii</i> in Pregnant Women in Meshkin-Shahr District, Northwestern Iran.\",\"authors\":\"Shabnam Asfaram, Zabih Zarei, Roghayeh Teimoorpour, Zahra Heidari, Sohrab Iranpour, Payman Azghani, Seyed Eghbal Motavallibashi, Zahra Rakhshidan, Zahra Khademi, Behnam Mohammadi-Ghalehbin, Shahram Habibzadeh, Soheila Molaei\",\"doi\":\"10.18502/ijpa.v20i1.18104\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>We aimed to investigate the serological and molecular characteristics of <i>Toxoplasma gondii</i> infection among pregnant women and their aborted placentas in Meshkin-Shahr City during 2019-2020.</p><p><strong>Methods: </strong>Blood samples of 210 pregnant women were evaluated for anti-<i>T. gondii</i> antibodies and related risk factors were determined. Also, the sera of aborted women and their buffy coats and aborted placenta tissues were used to detect anti-<i>Toxoplasma</i> antibodies and the parasite's DNA, respectively. The parasite genotypes were determined by the PCR-RFLP using the <i>SAG3</i> gene.</p><p><strong>Results: </strong>The overall prevalence of anti-<i>Toxoplasma</i> IgG was 24.3% and only 1% of participants were seropositive for the IgM antibody. There was a significant relationship between raw or unwashed vegetable consumption, contact with soil, vegetable/fruit washing type, and seropositivity (<i>P</i><0.05). During pregnancy, 4.7% of women encountered an abortion and 30% and 50% of cases were positive for IgG antibodies before and after abortion, respectively. Only two cases were IgM seropositive after abortion. In the avidity IgG test, 20% of cases showed low avidity. BLAST and phylogenetic analysis exhibited that all isolates belonged to the type III <i>T. gondii</i> genotype. Although two women with spontaneous abortions showed seropositivity for IgM <i>T. gondii</i> antibody, parasite DNA was detected in three cases.</p><p><strong>Conclusion: </strong>The seroprevalence of <i>Toxoplasma</i> infection is not high in pregnant women. Seropositive women are not safe from congenital transmission. <i>T. gondii</i> type III is the etiology of fetus infection in mothers with spontaneous abortion. It seems that screening and essential care are still necessary during pregnancy.</p>\",\"PeriodicalId\":14669,\"journal\":{\"name\":\"Iranian Journal of Parasitology\",\"volume\":\"20 1\",\"pages\":\"44-53\"},\"PeriodicalIF\":0.9000,\"publicationDate\":\"2025-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11978203/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Iranian Journal of Parasitology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.18502/ijpa.v20i1.18104\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"PARASITOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Iranian Journal of Parasitology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.18502/ijpa.v20i1.18104","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"PARASITOLOGY","Score":null,"Total":0}
Serological Evaluation and Molecular Genotyping of Toxoplasma gondii in Pregnant Women in Meshkin-Shahr District, Northwestern Iran.
Background: We aimed to investigate the serological and molecular characteristics of Toxoplasma gondii infection among pregnant women and their aborted placentas in Meshkin-Shahr City during 2019-2020.
Methods: Blood samples of 210 pregnant women were evaluated for anti-T. gondii antibodies and related risk factors were determined. Also, the sera of aborted women and their buffy coats and aborted placenta tissues were used to detect anti-Toxoplasma antibodies and the parasite's DNA, respectively. The parasite genotypes were determined by the PCR-RFLP using the SAG3 gene.
Results: The overall prevalence of anti-Toxoplasma IgG was 24.3% and only 1% of participants were seropositive for the IgM antibody. There was a significant relationship between raw or unwashed vegetable consumption, contact with soil, vegetable/fruit washing type, and seropositivity (P<0.05). During pregnancy, 4.7% of women encountered an abortion and 30% and 50% of cases were positive for IgG antibodies before and after abortion, respectively. Only two cases were IgM seropositive after abortion. In the avidity IgG test, 20% of cases showed low avidity. BLAST and phylogenetic analysis exhibited that all isolates belonged to the type III T. gondii genotype. Although two women with spontaneous abortions showed seropositivity for IgM T. gondii antibody, parasite DNA was detected in three cases.
Conclusion: The seroprevalence of Toxoplasma infection is not high in pregnant women. Seropositive women are not safe from congenital transmission. T. gondii type III is the etiology of fetus infection in mothers with spontaneous abortion. It seems that screening and essential care are still necessary during pregnancy.
期刊介绍：
Iranian Journal of Parasitology (IJP) is the official publication of Iranian Society of Parasitology (ISP) launched in 2006. The society was inaugurated in 1994 and pursues the improvement of the knowledge on the parasites and parasitic diseases, exchange of scientific knowledge with foreign societies, publicity activities, and consultation on the parasitic diseases, and intimate relationship among society members.
The main aims of the Journal are: contribution to the field of Parasitology, including all aspects of parasites and parasitic diseases (medical and veterinary) and related fields such as Entomology which may be submitted by scientists from Iran and all over the world.
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