Adnan Fadhel Al-Azaawie, Ahmed AbdulJabbar Suleiman, Mousa Jasim Mohammed
{"title":"揭示精子功能障碍中piwi相互作用rna与类固醇5 α还原酶2型之间的分子串扰。","authors":"Adnan Fadhel Al-Azaawie, Ahmed AbdulJabbar Suleiman, Mousa Jasim Mohammed","doi":"10.1016/j.xfss.2025.03.007","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>To investigate the correlation between piwi-interacting RNA (piRNA) expression and steroid 5 alpha reductase type 2 (SRD5A2) mRNA regulation in seminal fluid across various male infertility conditions (asthenozoospermia, oligozoospermia, and azoospermia).</p><p><strong>Design and subjects: </strong>This study included 88 male participants aged 20-40 years, categorized into infertility and normozoospermic groups.</p><p><strong>Exposure: </strong>Seminal fluid analysis and RNA extraction were performed to quantify SRD5A2 mRNA and selected piRNAs (hsa-piR-002528, hsa-piR-017183, hsa-piR-023244, and hsa-piR-023338) using qRT-PCR.</p><p><strong>Main outcome measures: </strong>Correlation analysis evaluated interactions between piRNA levels and SRD5A2 expression. Statistical significance was determined using analysis of variance and correlation coefficients.</p><p><strong>Results: </strong>Seminal Fluid Analysis: significant differences in seminal volume, sperm morphology, count, and motility were observed across infertility subtypes. Steroid 5 alpha reductase type 2 Expression: asthenozoospermia showed up-regulated SRD5A2 mRNA (Log2FC = 0.333), whereas oligozoospermia and azoospermia exhibited down-regulation (Log2FC = -0.470 and -0.688, respectively). Piwi-interacting RNA Expression: hsa-piR-002528 and hsa-piR-017183 were up-regulated in all infertility subtypes, whereas hsa-piR-023244 and hsa-piR-023338 exhibited subtype-specific expression patterns. Correlation Analysis: Steroid 5 alpha reductase type 2 mRNA negatively correlated with hsa-piR-002528 and hsa-piR-023338, suggesting regulatory interactions affecting sperm motility and count. Positive correlations were observed for hsa-piR-023244 in azoospermia, indicating potential roles in supporting spermatogenesis.</p><p><strong>Conclusions: </strong>Altered piRNA profiles and SRD5A2 expression are associated with male infertility subtypes. These findings highlight the regulatory role of piRNAs in spermatogenesis and their potential as biomarkers and therapeutic targets for male infertility.</p>","PeriodicalId":73012,"journal":{"name":"F&S science","volume":" ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Unveiling the molecular cross-talk between piwi-interacting RNAs and steroid 5 alpha reductase type 2 in sperm dysfunction.\",\"authors\":\"Adnan Fadhel Al-Azaawie, Ahmed AbdulJabbar Suleiman, Mousa Jasim Mohammed\",\"doi\":\"10.1016/j.xfss.2025.03.007\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>To investigate the correlation between piwi-interacting RNA (piRNA) expression and steroid 5 alpha reductase type 2 (SRD5A2) mRNA regulation in seminal fluid across various male infertility conditions (asthenozoospermia, oligozoospermia, and azoospermia).</p><p><strong>Design and subjects: </strong>This study included 88 male participants aged 20-40 years, categorized into infertility and normozoospermic groups.</p><p><strong>Exposure: </strong>Seminal fluid analysis and RNA extraction were performed to quantify SRD5A2 mRNA and selected piRNAs (hsa-piR-002528, hsa-piR-017183, hsa-piR-023244, and hsa-piR-023338) using qRT-PCR.</p><p><strong>Main outcome measures: </strong>Correlation analysis evaluated interactions between piRNA levels and SRD5A2 expression. Statistical significance was determined using analysis of variance and correlation coefficients.</p><p><strong>Results: </strong>Seminal Fluid Analysis: significant differences in seminal volume, sperm morphology, count, and motility were observed across infertility subtypes. Steroid 5 alpha reductase type 2 Expression: asthenozoospermia showed up-regulated SRD5A2 mRNA (Log2FC = 0.333), whereas oligozoospermia and azoospermia exhibited down-regulation (Log2FC = -0.470 and -0.688, respectively). Piwi-interacting RNA Expression: hsa-piR-002528 and hsa-piR-017183 were up-regulated in all infertility subtypes, whereas hsa-piR-023244 and hsa-piR-023338 exhibited subtype-specific expression patterns. Correlation Analysis: Steroid 5 alpha reductase type 2 mRNA negatively correlated with hsa-piR-002528 and hsa-piR-023338, suggesting regulatory interactions affecting sperm motility and count. Positive correlations were observed for hsa-piR-023244 in azoospermia, indicating potential roles in supporting spermatogenesis.</p><p><strong>Conclusions: </strong>Altered piRNA profiles and SRD5A2 expression are associated with male infertility subtypes. These findings highlight the regulatory role of piRNAs in spermatogenesis and their potential as biomarkers and therapeutic targets for male infertility.</p>\",\"PeriodicalId\":73012,\"journal\":{\"name\":\"F&S science\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2025-04-29\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"F&S science\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1016/j.xfss.2025.03.007\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"F&S science","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1016/j.xfss.2025.03.007","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Unveiling the molecular cross-talk between piwi-interacting RNAs and steroid 5 alpha reductase type 2 in sperm dysfunction.
Objective: To investigate the correlation between piwi-interacting RNA (piRNA) expression and steroid 5 alpha reductase type 2 (SRD5A2) mRNA regulation in seminal fluid across various male infertility conditions (asthenozoospermia, oligozoospermia, and azoospermia).
Design and subjects: This study included 88 male participants aged 20-40 years, categorized into infertility and normozoospermic groups.
Exposure: Seminal fluid analysis and RNA extraction were performed to quantify SRD5A2 mRNA and selected piRNAs (hsa-piR-002528, hsa-piR-017183, hsa-piR-023244, and hsa-piR-023338) using qRT-PCR.
Main outcome measures: Correlation analysis evaluated interactions between piRNA levels and SRD5A2 expression. Statistical significance was determined using analysis of variance and correlation coefficients.
Results: Seminal Fluid Analysis: significant differences in seminal volume, sperm morphology, count, and motility were observed across infertility subtypes. Steroid 5 alpha reductase type 2 Expression: asthenozoospermia showed up-regulated SRD5A2 mRNA (Log2FC = 0.333), whereas oligozoospermia and azoospermia exhibited down-regulation (Log2FC = -0.470 and -0.688, respectively). Piwi-interacting RNA Expression: hsa-piR-002528 and hsa-piR-017183 were up-regulated in all infertility subtypes, whereas hsa-piR-023244 and hsa-piR-023338 exhibited subtype-specific expression patterns. Correlation Analysis: Steroid 5 alpha reductase type 2 mRNA negatively correlated with hsa-piR-002528 and hsa-piR-023338, suggesting regulatory interactions affecting sperm motility and count. Positive correlations were observed for hsa-piR-023244 in azoospermia, indicating potential roles in supporting spermatogenesis.
Conclusions: Altered piRNA profiles and SRD5A2 expression are associated with male infertility subtypes. These findings highlight the regulatory role of piRNAs in spermatogenesis and their potential as biomarkers and therapeutic targets for male infertility.
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