Lorenzo Iuliano, Alessandro Marchese, Elisabetta Miserocchi, Eleonora Corbelli, Lucia Bongiovanni, Maurilio Ponzoni, Francesco Bandello, Marco Codenotti
{"title":"诊断性玻璃体切除术期间视网膜下灌洗:一种疑似玻璃体视网膜淋巴瘤细胞取样的辅助技术。","authors":"Lorenzo Iuliano, Alessandro Marchese, Elisabetta Miserocchi, Eleonora Corbelli, Lucia Bongiovanni, Maurilio Ponzoni, Francesco Bandello, Marco Codenotti","doi":"10.1097/ICB.0000000000001766","DOIUrl":null,"url":null,"abstract":"<p><strong>Purpose: </strong>Current diagnostic methods for vitreoretinal lymphoma (VRL) with predominant subretinal involvement exhibit limited sensitivity. We introduce a novel subretinal lavage technique intended to enhance diagnostic yields during vitrectomy procedures.</p><p><strong>Methods: </strong>During diagnostic vitrectomy, 0.1-0.3 ml of balanced saline solution (BSS) is injected into the subretinal space, creating a localized retinal detachment. Immediately thereafter, the diluted cellular suspension is gently aspirated manually using a 41-gauge extendable soft needle.</p><p><strong>Results: </strong>The technique was successfully performed in three patients without complications, consistently yielding approximately 0.3 ml of lymphoma cell-enriched fluid. Cytological analysis confirmed the presence of CD20-positive B lymphocytes and the MYD88 L265P mutation in all cases. Vitreous cytology was concurrently positive in two out of the three patients.</p><p><strong>Conclusions: </strong>Subretinal lavage is a safe, straightforward, and effective adjunct to standard vitreous biopsy, significantly improving diagnostic accuracy in VRL cases characterized by predominant subretinal infiltration. Implementation of this technique may lead to improved clinical decision-making and patient outcomes.</p>","PeriodicalId":53580,"journal":{"name":"Retinal Cases and Brief Reports","volume":" ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Subretinal lavage during diagnostic vitrectomy: an adjunctive technique for cell sampling in suspected vitreoretinal lymphoma.\",\"authors\":\"Lorenzo Iuliano, Alessandro Marchese, Elisabetta Miserocchi, Eleonora Corbelli, Lucia Bongiovanni, Maurilio Ponzoni, Francesco Bandello, Marco Codenotti\",\"doi\":\"10.1097/ICB.0000000000001766\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Purpose: </strong>Current diagnostic methods for vitreoretinal lymphoma (VRL) with predominant subretinal involvement exhibit limited sensitivity. We introduce a novel subretinal lavage technique intended to enhance diagnostic yields during vitrectomy procedures.</p><p><strong>Methods: </strong>During diagnostic vitrectomy, 0.1-0.3 ml of balanced saline solution (BSS) is injected into the subretinal space, creating a localized retinal detachment. Immediately thereafter, the diluted cellular suspension is gently aspirated manually using a 41-gauge extendable soft needle.</p><p><strong>Results: </strong>The technique was successfully performed in three patients without complications, consistently yielding approximately 0.3 ml of lymphoma cell-enriched fluid. Cytological analysis confirmed the presence of CD20-positive B lymphocytes and the MYD88 L265P mutation in all cases. Vitreous cytology was concurrently positive in two out of the three patients.</p><p><strong>Conclusions: </strong>Subretinal lavage is a safe, straightforward, and effective adjunct to standard vitreous biopsy, significantly improving diagnostic accuracy in VRL cases characterized by predominant subretinal infiltration. Implementation of this technique may lead to improved clinical decision-making and patient outcomes.</p>\",\"PeriodicalId\":53580,\"journal\":{\"name\":\"Retinal Cases and Brief Reports\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2025-04-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Retinal Cases and Brief Reports\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1097/ICB.0000000000001766\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Medicine\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Retinal Cases and Brief Reports","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1097/ICB.0000000000001766","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Medicine","Score":null,"Total":0}
Subretinal lavage during diagnostic vitrectomy: an adjunctive technique for cell sampling in suspected vitreoretinal lymphoma.
Purpose: Current diagnostic methods for vitreoretinal lymphoma (VRL) with predominant subretinal involvement exhibit limited sensitivity. We introduce a novel subretinal lavage technique intended to enhance diagnostic yields during vitrectomy procedures.
Methods: During diagnostic vitrectomy, 0.1-0.3 ml of balanced saline solution (BSS) is injected into the subretinal space, creating a localized retinal detachment. Immediately thereafter, the diluted cellular suspension is gently aspirated manually using a 41-gauge extendable soft needle.
Results: The technique was successfully performed in three patients without complications, consistently yielding approximately 0.3 ml of lymphoma cell-enriched fluid. Cytological analysis confirmed the presence of CD20-positive B lymphocytes and the MYD88 L265P mutation in all cases. Vitreous cytology was concurrently positive in two out of the three patients.
Conclusions: Subretinal lavage is a safe, straightforward, and effective adjunct to standard vitreous biopsy, significantly improving diagnostic accuracy in VRL cases characterized by predominant subretinal infiltration. Implementation of this technique may lead to improved clinical decision-making and patient outcomes.