马来西亚内生真菌Apiospora malaysiana与相关子囊菌的比较基因组学表明,适应生活方式的选择具有潜在的可持续纤维素分解活性。

IF 3.9 2区 生物学 Q1 GENETICS & HEREDITY
Shashi Kant, Sreyashi Das, Subhajeet Dutta, Kajal Mandal, Aditya Upadhyay, Aditya N Sarangi, Rajib Majumder, Sucheta Tripathy
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引用次数: 0

摘要

子囊菌真菌产生的碳水化合物活性酶在生物燃料工业中很有价值。对马来西亚内生真菌Apiospora malaysiana与其他7个密切相关的内生和致病生物高质量基因组的比较分析表明,效应物和致病性相关基因主要定位于快速进化的基因稀疏区,而不是保守区。这表明,快速进化的区域在宿主适应中起作用的双部基因组结构。内生真菌通过丰富降解纤维素、半纤维素、木质素和果胶的酶来适应植物的入侵。相比之下,我们观察到病原真菌,特别是N. oryzae,显示出次生代谢物生物合成和分解代谢的数量减少,反映了生活方式的适应。在病原菌种中存在排他的产孢基因簇可能表明它们的致病关系。有限的基因组可塑性和低杂合性在A. malaysiana是符合其主要的无性生活周期选择在实验室条件下。与在YPD培养基中培养的菌株相比,在纤维素培养基中培养的马来蓟马分泌组具有更多的纤维素酶活性。在纯纤维素培养基中差异上调的基因表现出强烈的纤维素降解活性和参与逃避宿主监测系统检测的基因。在具有理想理化性质的细菌表达系统中成功克隆和表达了选定的cazyases,这突出了马来西亚芽孢杆菌在可持续酶纤维素水解酶生产方面的生物技术潜力。这些发现使内生菌成为纤维素水解酶研究和更广泛的生物工业应用的宝贵资源。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Comparative genomics of Endophytic Fungi Apiospora malaysiana with related Ascomycetes indicates adaptation attuned to lifestyle choices with potential sustainable cellulolytic activity.

Ascomycetes fungi produce Carbohydrate-Active enzymes that are prized in the biofuel industry. Comparative genome analysis of endophytic fungus Apiospora malaysiana with seven other closely related high quality genomes of endophytic and pathogenic organisms reveal that effectors and pathogenicity-related genes are predominantly localized within rapidly evolving gene-sparse regions rather than in the conserved region. This suggests bipartite genome architecture where the rapidly evolving region plays a role in host adaptation. Endophytic fungi adapt to plant invasion by enriching enzymes that degrade cellulose, hemicellulose, lignin, and pectin. In contrast, we observed pathogenic fungi, especially N. oryzae, shows a reduced number of secondary metabolites biosysnthesis and catabolism, reflecting lifestyle adaptation. The presence of exclusive sporulating gene clusters in pathogen species could possibly indicate their pathogenic affiliation. Limited genome plasticity and low heterozygosity in A. malaysiana are in line with its predominant asexual life cycle choices in lab conditions. The secretome of A. malaysiana grown in cellulose only media had more cellulase activities when compared to cultures grown in YPD media. Genes that were differentially up-regulated in cellulose-only media exhibited strong cellulose-degrading activity and genes involved in evading detection by hosts surveillance system. Successful cloning and expression of selected CAZymes in bacterial expression systems with desirable physicochemical properties highlight the biotechnological potential of A. malaysiana for sustainable enzyme cellulolytic enzyme production. These findings position endophytes as valuable resources for cellulolytic enzyme research and broader bio-industrial applications.

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来源期刊
DNA Research
DNA Research 生物-遗传学
CiteScore
6.00
自引率
4.90%
发文量
39
审稿时长
4.5 months
期刊介绍: DNA Research is an internationally peer-reviewed journal which aims at publishing papers of highest quality in broad aspects of DNA and genome-related research. Emphasis will be made on the following subjects: 1) Sequencing and characterization of genomes/important genomic regions, 2) Comprehensive analysis of the functions of genes, gene families and genomes, 3) Techniques and equipments useful for structural and functional analysis of genes, gene families and genomes, 4) Computer algorithms and/or their applications relevant to structural and functional analysis of genes and genomes. The journal also welcomes novel findings in other scientific disciplines related to genomes.
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