{"title":"饲粮中白藜芦醇通过促进衰老公猪线粒体功能相关基因表达来改善精液质量。","authors":"Jing Lv, Wentao Zhang, Ruizhi Hu, Xizi Yang, Jiatai Gong, Siqi Ma, Hongkun Xiang, Xupeng Yuan, Hongfu Zhang, Xi He, Jianhua He, Rejun Fang, Shusong Wu","doi":"10.1093/jas/skaf150","DOIUrl":null,"url":null,"abstract":"<p><p>Boar semen quality is a significant factor affecting reproductive efficiency in modern farms, and resveratrol (RES) has the potential to reduce mouse sperm mortality in our previous studies. Here, we found that dietary supplementation of RES (500 mg/kg) delayed the decline in semen quality by increasing sperm motility and survivability in aging boars (P < 0.01, n = 6) by a 12-week trial. RES increased the testosterone level and total antioxidant capacity (P < 0.01), while decreasing the cortisol (P < 0.05) and malondialdehyde (MDA, P < 0.01) in serum. Immunofluorescence assay and electron microscope scanning revealed that RES reduced the levels of ROS, 8-hydroxy-2'-deoxyguanosine (8-OHDG), and MDA (P < 0.01) to alleviate oxidative damage in semen. Analysis of mitochondrial function-related genes indicated that RES improved mitochondrial structural integrity and mitochondrial membrane potential (MMP) (P < 0.01) by increasing the mRNA expression of PGC-1α, TFB1M, and TFB2M (P < 0.01). These results demonstrated that RES can enhance semen quality and antioxidant capacity potentially through improving sperm mitochondrial function in aging boars.</p>","PeriodicalId":14895,"journal":{"name":"Journal of animal science","volume":" ","pages":""},"PeriodicalIF":2.7000,"publicationDate":"2025-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Dietary resveratrol improves semen quality by promoting mitochondrial function related genes expression in aging boars.\",\"authors\":\"Jing Lv, Wentao Zhang, Ruizhi Hu, Xizi Yang, Jiatai Gong, Siqi Ma, Hongkun Xiang, Xupeng Yuan, Hongfu Zhang, Xi He, Jianhua He, Rejun Fang, Shusong Wu\",\"doi\":\"10.1093/jas/skaf150\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Boar semen quality is a significant factor affecting reproductive efficiency in modern farms, and resveratrol (RES) has the potential to reduce mouse sperm mortality in our previous studies. Here, we found that dietary supplementation of RES (500 mg/kg) delayed the decline in semen quality by increasing sperm motility and survivability in aging boars (P < 0.01, n = 6) by a 12-week trial. RES increased the testosterone level and total antioxidant capacity (P < 0.01), while decreasing the cortisol (P < 0.05) and malondialdehyde (MDA, P < 0.01) in serum. Immunofluorescence assay and electron microscope scanning revealed that RES reduced the levels of ROS, 8-hydroxy-2'-deoxyguanosine (8-OHDG), and MDA (P < 0.01) to alleviate oxidative damage in semen. Analysis of mitochondrial function-related genes indicated that RES improved mitochondrial structural integrity and mitochondrial membrane potential (MMP) (P < 0.01) by increasing the mRNA expression of PGC-1α, TFB1M, and TFB2M (P < 0.01). These results demonstrated that RES can enhance semen quality and antioxidant capacity potentially through improving sperm mitochondrial function in aging boars.</p>\",\"PeriodicalId\":14895,\"journal\":{\"name\":\"Journal of animal science\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":2.7000,\"publicationDate\":\"2025-05-04\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of animal science\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1093/jas/skaf150\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRICULTURE, DAIRY & ANIMAL SCIENCE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of animal science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1093/jas/skaf150","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}
Dietary resveratrol improves semen quality by promoting mitochondrial function related genes expression in aging boars.
Boar semen quality is a significant factor affecting reproductive efficiency in modern farms, and resveratrol (RES) has the potential to reduce mouse sperm mortality in our previous studies. Here, we found that dietary supplementation of RES (500 mg/kg) delayed the decline in semen quality by increasing sperm motility and survivability in aging boars (P < 0.01, n = 6) by a 12-week trial. RES increased the testosterone level and total antioxidant capacity (P < 0.01), while decreasing the cortisol (P < 0.05) and malondialdehyde (MDA, P < 0.01) in serum. Immunofluorescence assay and electron microscope scanning revealed that RES reduced the levels of ROS, 8-hydroxy-2'-deoxyguanosine (8-OHDG), and MDA (P < 0.01) to alleviate oxidative damage in semen. Analysis of mitochondrial function-related genes indicated that RES improved mitochondrial structural integrity and mitochondrial membrane potential (MMP) (P < 0.01) by increasing the mRNA expression of PGC-1α, TFB1M, and TFB2M (P < 0.01). These results demonstrated that RES can enhance semen quality and antioxidant capacity potentially through improving sperm mitochondrial function in aging boars.
期刊介绍：
The Journal of Animal Science (JAS) is the premier journal for animal science and serves as the leading source of new knowledge and perspective in this area. JAS publishes more than 500 fully reviewed research articles, invited reviews, technical notes, and letters to the editor each year.
Articles published in JAS encompass a broad range of research topics in animal production and fundamental aspects of genetics, nutrition, physiology, and preparation and utilization of animal products. Articles typically report research with beef cattle, companion animals, goats, horses, pigs, and sheep; however, studies involving other farm animals, aquatic and wildlife species, and laboratory animal species that address fundamental questions related to livestock and companion animal biology will be considered for publication.
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