Assessment of the Scolicidal Effect of Bile: An Ex Vivo Study.

Background: Approximately 25% of hepatic hydatid cysts rupture into the biliary tract. The precise effect of bile within the cyst on protoscoleces remains unclear. We aimed to elucidate the effect of bile on protoscoleces.

Methods: The contents of hydatid cysts from the livers of three sheep were aspirated under sterile conditions. The aspirated contents were divided into 50 separate Eppendorf tubes (5 cc). Samples from each tube were stained with 0.1% eosin Y. Pink stained protoscoleces were considered dead under light microscopy (×100). A total of 100 protoscoleces were counted in each sample, and the number of live and dead protoscoleces was recorded. The tubes were randomly divided into five groups. Group 1 served as the control, Group 2 received normal saline (NS), Group 3, received hypertonic saline, Group 4 received bile, and Group 4 received diluted bile. The number of live and dead protoscoleces was recorded at the end of the first and second hours.

Results: Compared to the initial count of live protoscoleces, the number of live protoscoleces increased at hours 1 and 2 in Groups 2 and 4. No live protoscoleces remained at hours 1 and 2 in Group 3. There was no significant change in Group 5. When compared to the control group, a significant increase in viability was observed only in Group 4 (P=0.001).

Conclusion: Bile of sheep does not exhibit scolicidal effects; rather, it positively affects protoscoleces by increasing viability.