基于HS-SPME-GC-MS的不同类型广南地茶香气挥发性物质气味活性值研究

IF 3.4 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Metabolites Pub Date : 2025-04-09 DOI:10.3390/metabo15040257
Ying Feng, Di Tian, Chaoliang Wang, Yong Huang, Yang Luo, Xiuqiong Zhang, Lei Li
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引用次数: 0

摘要

地徐茶是滇东南地区特色茶叶种质资源之一,也是珍贵的野生茶叶种质资源。背景:为了进一步开发地虚茶产品,提高其风味,本文研究了不同加工方法对地虚茶香气品质的影响。方法:采用HS-SPME结合GC-MS和多元统计分析对地味茶的香气品质进行综合分析。采用主成分分析(PCA)对检测到的挥发性物质进行处理,建立正交偏最小二乘判别分析(OPLS-DA)模型。我们通过计算气味活性值(OAV)来评估主要化合物对茶叶香气的贡献。结果:共鉴定出67个化合物。共筛选出27种主要芳香挥发性化合物(OAV bbb1),并在不同茶叶样品中鉴定出17种关键差异挥发性化合物,包括辛酸、d-柠檬醇、月桂烯、己醛、柠檬醛、β-环柠檬醛、反式-2-己烯醛、γ-壬烷内酯、β-离子酮、香叶丙酮、1,1,6-三甲基-1,2-二氢萘、香叶醇、水杨酸甲酯、芳樟醇、神经醇和7,11-二甲基-3-亚甲基-1,6,10-十二癸烯。结合OAV分析表明,花香是广南地虚茶品种的共同特征。此外,白茶也有一种芳香,而红茶,绿茶和竹筒茶都伴随着水果的香气。结论:综上所述,加工工艺通过改变挥发性香气化合物的种类和含量来调节不同类型茶叶的香气特性。这为今后茶叶生产资源的开发利用提供了理论依据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Aromatic Volatile Substances in Different Types of Guangnan Dixu Tea Based on HS-SPME-GC-MS Odor Activity Value.

Dixu tea is one of the characteristic tea germplasm resources of southeastern Yunnan, and is also a precious wild tea germplasm resource. Background: In order to further develop Dixu tea products and improve their flavor, this article studies the effects of different processing methods on the aroma quality of Dixu tea. Methods: A comprehensive analysis of the aroma quality of Diwei tea was conducted using HS-SPME combined with GC-MS and multivariate statistical analysis. A principal component analysis (PCA) was applied to process the detected volatile substances and an orthogonal partial least squares-discriminant analysis (OPLS-DA) model was established. We evaluated the contribution of major compounds in the tea aroma by calculating the odor activity value (OAV). Results: The results showed that a total of 67 compounds were identified. A total of 27 major aromatic volatile compounds (OAV > 1) were screened, and 17 key differential volatile compounds were identified in different tea samples, including octanoic acid, d-citrol, laurene, hexanal, citral, β-cyclic citral, trans-2-hexenal, γ-nonanolide, β-ionone, geranylacetone, 1,1,6-trimethyl-1,2-dihydronaphthalene, geraniol, methyl salicylate, linalool, nerolidol, and 7,11-dimethyl-3-methylene-1,6,10-dodecatriene. Combined with the OAV analysis, it is shown that a floral fragrance is a common feature of Guangnan Dixu tea varieties. In addition, white tea also has a fragrant aroma, while black tea, green tea, and bamboo tube tea are all accompanied by a fruity aroma. Conclusions: In summary, processing techniques regulate the aroma characteristics of various types of tea by changing the types and contents of volatile aroma compounds. This provides a theoretical basis for exploring and utilizing tea production resources in the future.

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来源期刊
Metabolites
Metabolites Biochemistry, Genetics and Molecular Biology-Molecular Biology
CiteScore
5.70
自引率
7.30%
发文量
1070
审稿时长
17.17 days
期刊介绍: Metabolites (ISSN 2218-1989) is an international, peer-reviewed open access journal of metabolism and metabolomics. Metabolites publishes original research articles and review articles in all molecular aspects of metabolism relevant to the fields of metabolomics, metabolic biochemistry, computational and systems biology, biotechnology and medicine, with a particular focus on the biological roles of metabolites and small molecule biomarkers. Metabolites encourages scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on article length. Sufficient experimental details must be provided to enable the results to be accurately reproduced. Electronic material representing additional figures, materials and methods explanation, or supporting results and evidence can be submitted with the main manuscript as supplementary material.
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