尿苷二磷酸(UDP)-糖基转移酶基因与阿维菌素耐药性相关的研究。

IF 1.8 2区 农林科学 Q2 ENTOMOLOGY
Guifeng Hao, Qing Chen, Ying Liu, Chunling Wu, Xingkui An, Ijiti Oluwole Gregory, Xiao Liang
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引用次数: 0

摘要

尿苷二磷酸(UDP)-糖基转移酶(UGT)是多种生物体内对亲脂性外源化合物进行糖基化的解毒酶。荨麻疹叶螨是危害农作物生产的重要害虫,在世界范围内存在严重的抗性问题。然而,UGT基因在荨麻疹耐药中的作用尚不清楚。本研究发现,实验室选择的耐药菌株(AbR)与敏感菌株(SS)相比产生了2万倍以上的耐药性。经阿维菌素处理后,AbR菌株中UGTs的活性和TuUGT201D3的转录显著高于SS,分子对接表明UGT201D3蛋白与阿维菌素具有较高的结合能力,提示两者之间可能存在相互作用。此外,敲低TuUGT201D3转录导致UGTs活性降低,AbR菌株在阿维菌素处理48 h后的死亡率(58.4%)显著高于对照(41.1%)。这些结果表明TuUGT201D3与荨麻疹耐阿维菌素相关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Characterization of a uridine diphosphate (UDP)-glycosyltransferase gene associated with abamectin resistance in two-spotted spider mite, Tetranychus urticae.

Uridine diphosphate (UDP)-glycosyltransferase (UGT) belongs to detoxification enzyme glycosylating lipophilic xenobiotic compounds in various living organisms. Tetranychus urticae is a notorious pest due to its significant threat to crop production and serious resistance problem worldwide. However, the function of UGT gene in contributing to pesticide resistance in T. urticae remained largely unknown. In this study, it was found that the laboratory selected abamectin-resistant (AbR) strain had developed over 20,000-fold resistance compared with the susceptible strain (SS). After being treated with abamectin, the activities of UGTs, and the transcription of TuUGT201D3 in the AbR strain were significantly higher than those in SS. Molecular docking indicated that the UGT201D3 protein exhibited high binding capacity with abamectin, suggesting the potential interaction between them. Furthermore, knock-down the transcription of TuUGT201D3 led to the decrease of activities of UGTs, in addition, the mortalities of AbR strain (58.4%) will significantly increase compared to control (41.1%) under 48 h of abamectin treatment. Those findings elucidated that TuUGT201D3 was correlated with abamectin resistance in T. urticae.

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来源期刊
CiteScore
3.90
自引率
9.10%
发文量
81
审稿时长
4-8 weeks
期刊介绍: Experimental and Applied Acarology publishes peer-reviewed original papers describing advances in basic and applied research on mites and ticks. Coverage encompasses all Acari, including those of environmental, agricultural, medical and veterinary importance, and all the ways in which they interact with other organisms (plants, arthropods and other animals). The subject matter draws upon a wide variety of disciplines, including evolutionary biology, ecology, epidemiology, physiology, biochemistry, toxicology, immunology, genetics, molecular biology and pest management sciences.
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