Ali Asghar Zarei, Sara Keshtgar, Masoud Haghani, Negar Firouzabadi, Mohammad Hossein Nasr-Esfahani
{"title":"NNC 55-0396和奎尼丁抑制CatSper和KSper通道对人精子功能的影响","authors":"Ali Asghar Zarei, Sara Keshtgar, Masoud Haghani, Negar Firouzabadi, Mohammad Hossein Nasr-Esfahani","doi":"10.30476/ijms.2024.102383.3528","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Calcium enters human sperm through the \"Cation Channel of Sperm\" (CatSper), while potassium ions exit via the sperm potassium channel (KSper). These two channels regulate intracellular calcium concentration ([Ca<sup>2+</sup>]<sub>i</sub>) and membrane potential. Our study aims to investigate and compare the contributions of these channels in capacitated sperm function.</p><p><strong>Methods: </strong></p><p><strong>Results: </strong>NNC and quinidine significantly decreased progressive sperm motility (P=0.001) and reduced sperm kinematics (P=0.001). NNC but not quinidine significantly decreased sperm survival (P=0.001), reduced [Ca<sup>2+</sup>]<sub>i</sub> in live spermatozoa (P=0.05), and induced the acrosomal reaction (P=0.012).</p><p><strong>Conclusion: </strong>Inhibition of KSper without effect on [Ca<sup>2+</sup>]<sub>i</sub> can inhibit sperm motility and increase mortality rate. It seems that the function of KSper is as vital as CatSper in human sperm physiology.</p>","PeriodicalId":14510,"journal":{"name":"Iranian Journal of Medical Sciences","volume":"50 3","pages":"187-196"},"PeriodicalIF":1.6000,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11992345/pdf/","citationCount":"0","resultStr":"{\"title\":\"Comparison between Inhibition of CatSper and KSper Channels with NNC 55-0396 and Quinidine on Human Sperm Function.\",\"authors\":\"Ali Asghar Zarei, Sara Keshtgar, Masoud Haghani, Negar Firouzabadi, Mohammad Hossein Nasr-Esfahani\",\"doi\":\"10.30476/ijms.2024.102383.3528\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Calcium enters human sperm through the \\\"Cation Channel of Sperm\\\" (CatSper), while potassium ions exit via the sperm potassium channel (KSper). These two channels regulate intracellular calcium concentration ([Ca<sup>2+</sup>]<sub>i</sub>) and membrane potential. Our study aims to investigate and compare the contributions of these channels in capacitated sperm function.</p><p><strong>Methods: </strong></p><p><strong>Results: </strong>NNC and quinidine significantly decreased progressive sperm motility (P=0.001) and reduced sperm kinematics (P=0.001). NNC but not quinidine significantly decreased sperm survival (P=0.001), reduced [Ca<sup>2+</sup>]<sub>i</sub> in live spermatozoa (P=0.05), and induced the acrosomal reaction (P=0.012).</p><p><strong>Conclusion: </strong>Inhibition of KSper without effect on [Ca<sup>2+</sup>]<sub>i</sub> can inhibit sperm motility and increase mortality rate. It seems that the function of KSper is as vital as CatSper in human sperm physiology.</p>\",\"PeriodicalId\":14510,\"journal\":{\"name\":\"Iranian Journal of Medical Sciences\",\"volume\":\"50 3\",\"pages\":\"187-196\"},\"PeriodicalIF\":1.6000,\"publicationDate\":\"2025-03-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11992345/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Iranian Journal of Medical Sciences\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.30476/ijms.2024.102383.3528\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"MEDICINE, GENERAL & INTERNAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Iranian Journal of Medical Sciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.30476/ijms.2024.102383.3528","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MEDICINE, GENERAL & INTERNAL","Score":null,"Total":0}
Comparison between Inhibition of CatSper and KSper Channels with NNC 55-0396 and Quinidine on Human Sperm Function.
Background: Calcium enters human sperm through the "Cation Channel of Sperm" (CatSper), while potassium ions exit via the sperm potassium channel (KSper). These two channels regulate intracellular calcium concentration ([Ca2+]i) and membrane potential. Our study aims to investigate and compare the contributions of these channels in capacitated sperm function.
Methods:
Results: NNC and quinidine significantly decreased progressive sperm motility (P=0.001) and reduced sperm kinematics (P=0.001). NNC but not quinidine significantly decreased sperm survival (P=0.001), reduced [Ca2+]i in live spermatozoa (P=0.05), and induced the acrosomal reaction (P=0.012).
Conclusion: Inhibition of KSper without effect on [Ca2+]i can inhibit sperm motility and increase mortality rate. It seems that the function of KSper is as vital as CatSper in human sperm physiology.
期刊介绍：
The Iranian Journal of Medical Sciences (IJMS) is an international quarterly biomedical publication, which is sponsored by Shiraz University of Medical Sciences. The IJMS intends to provide a scientific medium of communication for researchers throughout the globe. The journal welcomes original clinical articles as well as clinically oriented basic science research experiences on prevalent diseases in the region and analysis of various regional problems.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
