alcian blue-periodic acid schiff, Ayoub-Shklar和改性papanicolaou作为特殊染色剂定性分析口腔鳞状细胞癌中的角蛋白。

Q3 Medicine
Ravi Jyoti, Wadhwan Vijay, Sharma Preeti, Yadav Lalita
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引用次数: 0

摘要

背景:角化的形态学特征可以影响组织病理学诊断。常规血红素-伊红(H&E)染色在中度分化口腔鳞状细胞癌(OSCC)中有其局限性。目的:本研究的目的是评价和比较常规H&E染色法、阿利新蓝-周期酸希夫(AB-PAS)染色法、Ayoub-Shklar染色法和改良Papanicolaou (PAP)染色法在OSCC病例中角蛋白的染色和鉴定特点。材料与方法:将30例OSCC作为实验组,将20例高分化鳞状细胞癌(WDSCC)、10例中分化鳞状细胞癌(MDSCC)和10例正常角化口腔黏膜(NKOM)作为对照组。选择经组织病理学诊断的OSCC病例,分别制作组织切片。统计分析采用费雪精确检验。结果和结论:我们观察到在WDSCC中Ayoub-Shklar染色强度更大,在MDSCC中修饰PAP和AB-PAS染色的切片中,修饰PAP在WDSCC中染色的特异性更大,在MDSCC中修饰PAP在AB-PAS和Ayoub-Shklar染色的切片中染色的特异性更大。我们认为,AB-PAS, Ayoub-Shklar和改良PAP染色等特殊染色可以证明是一种简单的方法,可以从组织学上检测角蛋白的存在和模式。oscc修饰的PAP染色对角蛋白珠和单个细胞角化的鉴别效果较好。它可以作为H&E的替代染色,用于基于角化的OSCC组织学分级。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Qualitative analysis of keratin in oral squamous cell carcinoma using alcian blue-periodic acid schiff, Ayoub-Shklar, and modified papanicolaou as special stains.

Background: The morphological features of keratinisation can impact histopathological diagnosis. Routine haematoxylin and eosin (H&E) stain has its limitations in moderately differentiated cases of oral squamous cell carcinoma (OSCC).

Aims: The aim of this study was to evaluate and compare the characteristics of staining and identification of keratin by routine H&E stain, Alcian Blue-Periodic Acid Schiff (AB-PAS), Ayoub-Shklar, and Modified Papanicolaou (PAP) stain in OSCC cases.

Material and methods: Overall, 30 cases of OSCC were included in experimental groups, and 20 cases of well-differentiated squamous cell carcinoma (WDSCC) and ten cases of moderately differentiated squamous cell carcinoma (MDSCC) and ten cases of normal keratinised oral mucosa (NKOM) were included in the control group. Histopathologicaly diagnosed cases of OSCC were selected, and tissue sections from each were prepared. Fisher's exact test was used for the statistical analysis.

Results and conclusions: We observed that the staining intensity was greater in Ayoub-Shklar in WDSCC and in MDSCC followed by modified PAP and AB-PAS-stained sections and the staining specificity was greater in modified PAP in WDSCC and in MDSCC followed by AB-PAS and Ayoub-Shklar in stained sections. We conclude that special stains like AB-PAS, Ayoub-Shklar, and modified PAP stain can prove to be a simple solution to detect the presence and pattern of keratin histologically. The efficacy of distinct identification of keratin pearls and individual cell keratinisation in OSCC-modified PAP stain is better. It can be used as alternative stain to H&E for histological grading of OSCC based on keratinisation.

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来源期刊
Journal of Oral and Maxillofacial Pathology
Journal of Oral and Maxillofacial Pathology Medicine-Otorhinolaryngology
CiteScore
1.40
自引率
0.00%
发文量
115
期刊介绍: The journal of Oral and Maxillofacial Pathology [ISSN:print-(0973-029X, online-1998-393X)] is a tri-annual journal published on behalf of “The Indian Association of Oral and Maxillofacial Pathologists” (IAOMP). The publication of JOMFP was started in the year 1993. The journal publishes papers on a wide spectrum of topics associated with the scope of Oral and Maxillofacial Pathology, also, ensuring scientific merit and quality. It is a comprehensive reading material for the professionals who want to upgrade their diagnostic skills in Oral Diseases; allows exposure to newer topics and methods of research in the Oral-facial Tissues and Pathology. New features allow an open minded thinking and approach to various pathologies. It also encourages authors to showcase quality work done by them and to compile relevant cases which are diagnostically challenging. The Journal takes pride in maintaining the quality of articles and photomicrographs.
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