P A Semizhon, E P Scheslenok, N A Dubkov, E A Sukhotskaya, K A Stolbunova, I V Popov, I V Popov, A Y Alekseev, E Kabwe, Y N Davidyuk
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引用次数: 0
摘要
导语：白俄罗斯共和国监测肾综合征出血热（HFRS）病原体是必要和相关的，因为近年来人群中HFRS病例数量有所增加，病原体的遗传特征仍未确定。研究目的：鉴定在白俄罗斯共和国境内流行的正汉坦病毒并确定其遗传特征。材料和方法：采用实时PCR方法，采用«Belar-GLPS-PCR/RV»检测系统，对在白俄罗斯共和国境内捕获的613份小型哺乳动物样本进行筛选。阳性样品采用Sanger法测序。使用Lasergene软件包（DNASTAR， USA）和MEGA 11中的MegAlign程序进行比较和系统发育分析。结果：初筛pcr阳性32份（5.2%），其中普马拉病毒（PUUV）阳性24份，多布拉瓦-贝尔格莱德病毒（DOBV）阳性8份。对PUUV的3个m段序列、DOBV的2个291碱基对（bp） m段序列和1个348 bp s段序列进行了测序。比较和系统发育分析表明，所鉴定的PUUV序列属于俄罗斯遗传谱系，与莫斯科和库尔斯克地区常见的菌株属于同一亚谱系。所鉴定的DOBV序列与来自俄罗斯欧洲部分中部地区的菌株关系最密切。结论：分子生物学分析结果表明，PUUV在白俄罗斯境内流行，分布广泛。同时，在共和国的四个地区发现了DOBV，这表明该HFRS病原体的范围扩大了。在白俄罗斯共和国，首次获得了正汉坦病毒的核苷酸序列，并对其进行了分子遗传分析。
[Identification of orthohantaviruses detected for the first time in the Republic of Belarus].
Introduction: Monitoring of hemorrhagic fever with renal syndrome (HFRS) pathogens in the Republic of Belarus is necessary and relevant, since the number of HFRS cases in the population has increased in recent years, and genetic characteristics of the pathogens remain unidentified.
Aim of the study: Identification of orthohantaviruses circulating in the territory of the Republic of Belarus and defining of their genetic characteristics.
Materials and methods: Screening of 613 samples from small mammals caught in the territory of the Republic of Belarus was carried out by the real time PCR method using the test system «Belar-GLPS-PCR/RV». Positive samples were sequenced by the Sanger method. Comparative and phylogenetic analysis was carried out using the MegAlign programs from the Lasergene package (DNASTAR, USA) and MEGA 11.
Results: The primary screening yielded 32 PCR-positive samples (5.2%), of which 24 belonged to Puumala virus (PUUV) and 8 to Dobrava-Belgrade virus (DOBV). Three nucleotide sequences of the M-segment region of PUUV, two sequences of the 291-base pair (bp) M-segment region and one sequence of the 348-bp S-segment region of DOBV were sequenced. Comparative and phylogenetic analysis showed that the identified PUUV sequences belong to the Russian genetic lineage, to the same sublineage as the strains common in the Moscow and Kursk regions. The identified DOBV ssequences demonstrated the closest relationship to the strains from the central region of the European part of Russia.
Conclusion: The results of molecular biological analysis showed that PUUV circulates in the territory of the Republic of Belarus and is widespread. At the same time, DOBV was detected in four regions of the republic, which indicates an expansion of the range of this HFRS pathogen. In the Republic of Belarus, nucleotide sequences of orthohantaviruses were obtained for the first time and their molecular genetic analysis was carried out.
期刊介绍：
The journal deals with advances in virology in Russia and abroad. It publishes papers dealing with investigations of viral diseases of man, animals and plants, the results of experimental research on different problems of general and special virology. The journal publishes materials are which promote introduction into practice of the achievements of the virological science in the eradication and incidence reduction of infectious diseases, as well as their diagnosis, treatment and prevention. The reader will find a description of new methods of investigation, new apparatus and devices.
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