Synthesis and Characterization of Fe3O4/Chitosan/TiO2 Nanoparticle Novel Nanocarrier and Investigation of In Vitro Release of Vancomycin on HeLa Cancer Cell.

Nanocarrier systems have gained significant attention in recent decades as an alternative to conventional drug delivery methods, which often suffer from various limitations. In this study, Fe₃O₄/chitosan/TiO₂ nanoparticles were synthesized as a novel nanocarrier for targeted drug delivery. The loading efficiency and controlled release behavior of vancomycin from the nanocarrier were evaluated under in vitro conditions using HeLa cancer cells. The in vitro study of the drug release behavior showed that the implementation of a titania coating significantly diminishes the drug release rate. Specifically, approximately 90 ± 0.2% of the drug is released over a period of 16 h for samples without the titania coating, while samples with the coating exhibit a release time of 25 h. The MTT assay indicates that the application of TiO₂ nanoparticles on the nanocarrier resulted in a decrease in cell viability from 90 ± 3% to 50 ± 2% at concentrations of 100 μg/mL and 500 μg/mL, respectively. These findings highlight the potential of the Fe₃O₄/chitosan/TiO₂ nanocarrier as an efficient system for controlled and targeted drug delivery applications.