Maribel Maldonado-Muñiz, M G Nieto-López, Mireya Tapia-Salazar, Bruno Gómez-Gil, A Guerrero, Rodolfo Lozano-Olvera, Lucia Elizabeth Cruz-Suarez, Sonia A Soto-Rodriguez
{"title":"饲料纳米银颗粒对凡纳滨对虾生长、组织病理学、粪便微生物组和急性肝胰腺坏死病生存的影响","authors":"Maribel Maldonado-Muñiz, M G Nieto-López, Mireya Tapia-Salazar, Bruno Gómez-Gil, A Guerrero, Rodolfo Lozano-Olvera, Lucia Elizabeth Cruz-Suarez, Sonia A Soto-Rodriguez","doi":"10.3354/dao03848","DOIUrl":null,"url":null,"abstract":"<p><p>This study examines acute hepatopancreatic necrosis disease (AHPND) in shrimp farming, caused by specific strains of Vibrio (Vp AHPND+), and evaluates the potential of silver and silver chloride nanoparticles (Ag/AgCl NP) synthesised from marine resources as an antibacterial additive in shrimp feed. Penaeus vannamei juveniles were fed diets supplemented with 0, 10, 100, and 1000 mg Ag/AgCl per kg for 20 d. The highest weight gain (%WG) was observed in individuals consuming the 100 mg Ag kg-1 diet, with a 104.27 ± 4.11% increase. Histopathological analysis revealed that 10 and 100 mg Ag kg-1 improved hepatopancreas (HP) ultrastructure, whereas 1000 mg Ag kg-1 caused severe lesions, including cell necrosis. After infection with Vp AHPND+, survival rates increased from 27% in the control group to 78% in juveniles fed the 100 mg Ag kg-1 diet for 7 d. Microbiome analysis showed no significant changes in alpha diversity due to Ag/AgCl NP doses, although a notable shift was observed during the depuration phase. These findings highlight the potential of Ag/AgCl NP as a dietary supplement to improve growth, health, and disease resistance in shrimp farming, emphasising the importance of precise dosing to maximize benefits while minimising risks.</p>","PeriodicalId":11252,"journal":{"name":"Diseases of aquatic organisms","volume":"162 ","pages":"35-49"},"PeriodicalIF":1.1000,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Dietary silver nanoparticle effects on Penaeus vannamei growth, histopathology, faecal microbiome, and acute hepatopancreatic necrosis disease survival.\",\"authors\":\"Maribel Maldonado-Muñiz, M G Nieto-López, Mireya Tapia-Salazar, Bruno Gómez-Gil, A Guerrero, Rodolfo Lozano-Olvera, Lucia Elizabeth Cruz-Suarez, Sonia A Soto-Rodriguez\",\"doi\":\"10.3354/dao03848\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>This study examines acute hepatopancreatic necrosis disease (AHPND) in shrimp farming, caused by specific strains of Vibrio (Vp AHPND+), and evaluates the potential of silver and silver chloride nanoparticles (Ag/AgCl NP) synthesised from marine resources as an antibacterial additive in shrimp feed. Penaeus vannamei juveniles were fed diets supplemented with 0, 10, 100, and 1000 mg Ag/AgCl per kg for 20 d. The highest weight gain (%WG) was observed in individuals consuming the 100 mg Ag kg-1 diet, with a 104.27 ± 4.11% increase. Histopathological analysis revealed that 10 and 100 mg Ag kg-1 improved hepatopancreas (HP) ultrastructure, whereas 1000 mg Ag kg-1 caused severe lesions, including cell necrosis. After infection with Vp AHPND+, survival rates increased from 27% in the control group to 78% in juveniles fed the 100 mg Ag kg-1 diet for 7 d. Microbiome analysis showed no significant changes in alpha diversity due to Ag/AgCl NP doses, although a notable shift was observed during the depuration phase. These findings highlight the potential of Ag/AgCl NP as a dietary supplement to improve growth, health, and disease resistance in shrimp farming, emphasising the importance of precise dosing to maximize benefits while minimising risks.</p>\",\"PeriodicalId\":11252,\"journal\":{\"name\":\"Diseases of aquatic organisms\",\"volume\":\"162 \",\"pages\":\"35-49\"},\"PeriodicalIF\":1.1000,\"publicationDate\":\"2025-05-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Diseases of aquatic organisms\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.3354/dao03848\",\"RegionNum\":4,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"FISHERIES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Diseases of aquatic organisms","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.3354/dao03848","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"FISHERIES","Score":null,"Total":0}
引用次数: 0
摘要
本研究考察了虾养殖中由特定弧菌(Vp AHPND+)引起的急性肝胰腺坏死病(AHPND),并评估了从海洋资源合成的银和氯化银纳米颗粒(Ag/AgCl NP)作为虾饲料抗菌添加剂的潜力。分别饲喂0、10、100和1000 mg Ag/AgCl / kg的饲料,饲喂20 d。饲料中添加100 mg Ag/AgCl / kg的个体增重(%WG)最高,为104.27±4.11%。组织病理学分析显示,10和100 mg Ag kg-1可改善肝胰腺(HP)超微结构,而1000 mg Ag kg-1可引起包括细胞坏死在内的严重病变。在感染Vp AHPND+后,喂食100 mg Ag kg-1饲料7 d的幼鱼存活率从对照组的27%增加到78%。微生物组分析显示,Ag/AgCl NP剂量对α多样性没有显著影响,尽管在净化阶段观察到显著变化。这些发现强调了Ag/AgCl NP作为一种膳食补充剂在改善虾类养殖中的生长、健康和抗病能力方面的潜力,强调了精确剂量的重要性,以最大限度地提高效益,同时将风险降至最低。
Dietary silver nanoparticle effects on Penaeus vannamei growth, histopathology, faecal microbiome, and acute hepatopancreatic necrosis disease survival.
This study examines acute hepatopancreatic necrosis disease (AHPND) in shrimp farming, caused by specific strains of Vibrio (Vp AHPND+), and evaluates the potential of silver and silver chloride nanoparticles (Ag/AgCl NP) synthesised from marine resources as an antibacterial additive in shrimp feed. Penaeus vannamei juveniles were fed diets supplemented with 0, 10, 100, and 1000 mg Ag/AgCl per kg for 20 d. The highest weight gain (%WG) was observed in individuals consuming the 100 mg Ag kg-1 diet, with a 104.27 ± 4.11% increase. Histopathological analysis revealed that 10 and 100 mg Ag kg-1 improved hepatopancreas (HP) ultrastructure, whereas 1000 mg Ag kg-1 caused severe lesions, including cell necrosis. After infection with Vp AHPND+, survival rates increased from 27% in the control group to 78% in juveniles fed the 100 mg Ag kg-1 diet for 7 d. Microbiome analysis showed no significant changes in alpha diversity due to Ag/AgCl NP doses, although a notable shift was observed during the depuration phase. These findings highlight the potential of Ag/AgCl NP as a dietary supplement to improve growth, health, and disease resistance in shrimp farming, emphasising the importance of precise dosing to maximize benefits while minimising risks.
期刊介绍:
DAO publishes Research Articles, Reviews, and Notes, as well as Comments/Reply Comments (for details see DAO 48:161), Theme Sections and Opinion Pieces. For details consult the Guidelines for Authors. Papers may cover all forms of life - animals, plants and microorganisms - in marine, limnetic and brackish habitats. DAO''s scope includes any research focusing on diseases in aquatic organisms, specifically:
-Diseases caused by coexisting organisms, e.g. viruses, bacteria, fungi, protistans, metazoans; characterization of pathogens
-Diseases caused by abiotic factors (critical intensities of environmental properties, including pollution)-
Diseases due to internal circumstances (innate, idiopathic, genetic)-
Diseases due to proliferative disorders (neoplasms)-
Disease diagnosis, treatment and prevention-
Molecular aspects of diseases-
Nutritional disorders-
Stress and physical injuries-
Epidemiology/epizootiology-
Parasitology-
Toxicology-
Diseases of aquatic organisms affecting human health and well-being (with the focus on the aquatic organism)-
Diseases as indicators of humanity''s detrimental impact on nature-
Genomics, proteomics and metabolomics of disease-
Immunology and disease prevention-
Animal welfare-
Zoonosis