坏死下垂和免疫浸润在原发性血小板增多中的作用。

IF 2.5 3区 生物学
Guangming Li, Ying Guo, Yuanyuan Zhang
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引用次数: 0

摘要

背景:坏死性上睑下垂是最近发现的一种程序性细胞死亡形式,与多种肿瘤和非肿瘤疾病的发病机制有关。然而,坏死性上睑塌陷在原发性血小板增多症(ET)中的作用尚不清楚,这是一种典型的骨髓增生性肿瘤。材料与方法:采用生物信息学方法结合临床样本的qRT-PCR分析,确定坏死下垂在ET中的作用。招募GSE57793和GSE26049数据集,基于差异基因鉴定、坏死性下垂基因集和数据机器学习,鉴定坏死性下垂差异表达基因。利用富集分析(富集分析GSEA)评价ET的基因富集信号通路,利用免疫浸润分析探讨ET中免疫细胞浸润的丰度,研究坏死下垂差异基因与免疫细胞浸润的相关性。结果:在坏死凋亡通路中发现了5个显著富集的坏死凋亡基因,包括CHMP1B、FTH1、HSP90AB1、IL1A和RBCK1。在ET中发现了Th1/Th17细胞的侵袭失衡,差异坏死下垂基因与多个免疫细胞的浸润呈正相关。ET有明显的坏死下垂,在坏死性凋亡通路中富集,与免疫浸润有关。结论:坏死性上睑下垂可能通过刺激免疫浸润和免疫反应来驱动ET的进展。研究结果为今后研究ET的治疗机制和治疗策略提供了新的思路。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Role of necroptosis and immune infiltration in essential thrombocytosis.

Background: Necroptosis, a recently identified form of programmed cell death involved in the pathogenesis of a variety of tumor and non-tumor diseases. Nevertheless, the function of necroptosis in essential thrombocytosis (ET) remains unclear, which is a classic myeloproliferative tumor.

Materials and methods: The role of necroptosis in ET was determined via bioinformatics combined with qRT-PCR analysis of clinical samples. GSE57793 and GSE26049 datasets were recruited to identify necroptosis differentially expressed genes based on differential gene identification, necroptosis gene sets and data machine learning. Enrichment analysis (GSEA) was used to evaluate the gene enrichment signaling pathway of ET, immune infiltration analysis was used to explore the abundance of immune cell infiltration in ET, and the correlation between necroptosis differential genes and immune cell infiltration was studied.

Results: Five necroptosis genes were recognized to be remarkably enriched in the necroptosis pathway, including CHMP1B, FTH1, HSP90AB1, IL1A, and RBCK1. The imbalance of invasion of Th1/Th17 cells was identified in ET, and the differential necroptosis gene was positively correlated with the infiltration of multiple immune cells. There is significant necroptosis in ET, which is enriched in the necrotizing apoptotic pathway, and is associated with immune infiltration.

Conclusions: Necroptosis might drive the progression of ET via stimulating immune infiltration and immune responses. The findings bring new insights into the treatment mechanism and treatment strategy of ET in the future.

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来源期刊
Hereditas
Hereditas Biochemistry, Genetics and Molecular Biology-Genetics
CiteScore
3.80
自引率
3.70%
发文量
0
期刊介绍: For almost a century, Hereditas has published original cutting-edge research and reviews. As the Official journal of the Mendelian Society of Lund, the journal welcomes research from across all areas of genetics and genomics. Topics of interest include human and medical genetics, animal and plant genetics, microbial genetics, agriculture and bioinformatics.
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