使用自动电化学发光免疫分析技术在唾液和血液激素浓度之间的关联:挑战和缺陷。

IF 2.6 4区 医学 Q2 PHYSIOLOGY
Natalie J Hardaker, Patria A Hume, Stacy T Sims, Tom Stewart, Doug A King
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引用次数: 0

摘要

基于血液的测量被认为是评估类固醇激素浓度的金标准,特别是在女性特异性研究中确认激素谱。然而,唾液取样有几个优点:它可以由参与者独立收集,侵入性较小,可能是一个更省时、更经济的解决方案。本初步研究利用自动电化学发光免疫分析技术探讨了唾液和血浆激素(雌激素、黄体酮和皮质醇)浓度之间的关系。8名健康、体力活跃、月经周期规律的女性提供:(1)基于app的每日月经跟踪数据;(2)每日唾液样本;(3) 2份血样,分别在月经周期的第3 ~第5个月经周期的不同时间点,采用逆向计算方法进行估计。使用重复测量的相关性分析了唾液和血液激素浓度之间的关系。孕酮和雌激素与血浆和唾液测量呈正相关;(rm = 0.996, p m = 0.705, p = 0.0507)。皮质醇的相关性不显著(rm = 0.245, p = 0.526)。随着进一步的实验验证和唾液参考范围的建立,电化学发光免疫分析技术可能可用于唾液中孕酮和雌激素的定量测定,并可用于参与者内监测随时间变化的研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Association between salivary and blood hormone concentrations using an automated electrochemiluminescence immunoassay technique: Challenges and pitfalls.

Blood-based measures are considered the gold standard for evaluating steroid hormone concentrations, particularly for confirmation of hormone profiles in female-specific research. However, saliva sampling offers several advantages: it can be collected independently by participants, is less invasive and might be a more time- and cost-effective solution. This preliminary study explored associations between salivary and blood plasma hormone (oestrogen, progesterone and cortisol) concentrations using an automated electrochemiluminescence immunoassay technique. Eight healthy, physically active females with a regular natural menstrual cycle provided: (1) daily app-based menstrual tracking data; (2) daily saliva samples; and (3) two blood samples, each at different time points in the third to fifth menstrual cycle estimated using the backward calculation method. Associations between saliva and blood hormone concentrations were analysed using repeated-measures correlations. Progesterone and oestrogen showed positive associations between blood plasma and salivary measures; (r= 0.996, p < 0.0001 and r= 0.705, p = 0.0507, respectively). Weak non-significant associations for cortisol (r= 0.245, p = 0.526) were found. With further work to validate the assay and develop salivary reference ranges, the electrochemiluminescence immunoassay technique might be feasible for use in quantitative determination of progesterone and oestrogen in saliva and have application in research for within-participant monitoring of changes over time.

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来源期刊
Experimental Physiology
Experimental Physiology 医学-生理学
CiteScore
5.10
自引率
3.70%
发文量
262
审稿时长
1 months
期刊介绍: Experimental Physiology publishes research papers that report novel insights into homeostatic and adaptive responses in health, as well as those that further our understanding of pathophysiological mechanisms in disease. We encourage papers that embrace the journal’s orientation of translation and integration, including studies of the adaptive responses to exercise, acute and chronic environmental stressors, growth and aging, and diseases where integrative homeostatic mechanisms play a key role in the response to and evolution of the disease process. Examples of such diseases include hypertension, heart failure, hypoxic lung disease, endocrine and neurological disorders. We are also keen to publish research that has a translational aspect or clinical application. Comparative physiology work that can be applied to aid the understanding human physiology is also encouraged. Manuscripts that report the use of bioinformatic, genomic, molecular, proteomic and cellular techniques to provide novel insights into integrative physiological and pathophysiological mechanisms are welcomed.
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