植物致病性γ变形菌原生质粒的比较基因组学研究。

IF 3.9 2区 生物学 Q1 GENETICS & HEREDITY
Miriam Urriza, Giulio Dimaria, Luiz Orlando de Oliveira, Vittoria Catara, Jesús Murillo
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引用次数: 0

摘要

质粒是植物致病性γ变形菌(PPG)进化和适应的关键,但其多样性和功能贡献仍未得到充分研究。在这里,比较基因组学揭示了质粒大小、复制子类型、流动性和遗传含量在PPG中的广泛差异。大多数质粒都很小(
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Comparative genomics of native plasmids from plant pathogenic Gammaproteobacteria.

Plasmids are key in the evolution and adaptation of plant pathogenic Gammaproteobacteria (PPG), yet their diversity and functional contributions remain underexplored. Here, comparative genomics revealed extensive variation in plasmid size, replicon types, mobility, and genetic content across PPG. Most plasmids are small (<200 kb), except in Pantoea, exhibiting high coding densities (76-78%). Five ancestral replicon types were identified across multiple orders, indicating vertical descent yet efficient horizontal transfer across taxa, although with limited genetic conservation. Virulence plasmids are widespread (56-68%) but differ in virulence gene content across orders: type III effector (T3E) genes are common in Pseudomonas and Xanthomonas, but rare in Enterobacterales and Xylella, aligning with their smaller effector repertoires. Plasmids frequently carry regulatory genes, highlighting their role in bacterial phenotype modulation. Distinct patterns were observed among orders: Enterobacterales plasmids often harbor thiamine biosynthesis operons and transcriptional regulators but lack post-transcriptional regulators, while Pseudomonas and Xanthomonas plasmids are highly mobile, enriched in T3E genes, and exhibit high insertion sequence densities, fostering DNA mobility. Resistance to ultraviolet light is common, but not to antimicrobial compounds. These findings highlight the dynamic role of plasmids in spreading adaptive traits, shaping virulence, and driving the evolution of plant pathogenic bacteria.

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来源期刊
DNA Research
DNA Research 生物-遗传学
CiteScore
6.00
自引率
4.90%
发文量
39
审稿时长
4.5 months
期刊介绍: DNA Research is an internationally peer-reviewed journal which aims at publishing papers of highest quality in broad aspects of DNA and genome-related research. Emphasis will be made on the following subjects: 1) Sequencing and characterization of genomes/important genomic regions, 2) Comprehensive analysis of the functions of genes, gene families and genomes, 3) Techniques and equipments useful for structural and functional analysis of genes, gene families and genomes, 4) Computer algorithms and/or their applications relevant to structural and functional analysis of genes and genomes. The journal also welcomes novel findings in other scientific disciplines related to genomes.
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