九味清脂膏治疗非酒精性脂肪肝的机理评价:网络药理学见解及实验验证。

IF 2.5 3区 生物学
Qinlei Chen, Qianfeng Hu, Fan Zhang, Weiting Lu, Zheng Yuan, Fei Qiao
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引用次数: 0

摘要

背景:九味清脂膏(JWQZG)是一种中药制剂,在中国被广泛用于治疗非酒精性脂肪性肝病(NAFLD)。目的:本研究旨在阐明金汤清心汤治疗NAFLD的作用机制。材料与方法:采用网络药理学方法,预测复方净身健脾方治疗NAFLD的可能机制。在体内实验中,C57BL/6J小鼠先饲喂高脂饲料(HFD) 16周,然后分别以1.85、3.7和7.4 g/kg/d的三种剂量(1.85、3.7和7.4 g/kg/d)或二甲双胍(150 mg/kg/d)治疗8周。体外研究将HepG2细胞暴露于0.5 mM棕榈酸(PA) 24小时建立NAFLD模型,然后再暴露于含三种浓度jwqzg的血清24小时。采用Western blot分析关键信号通路成分的表达水平。结果:网络药理学分析结果确定胰岛素信号通路是JWQZG对NAFLD保护作用的潜在介质。肝脂变性显著降低,胰岛素抵抗明显改善。与NAFLD模型组相比,胰岛素信号通路中关键成分的表达增强,包括胰岛素受体底物1 (IRS1)、磷酸化PI3K (p-PI3K)、磷酸化AKT (p-AKT)和磷酸化GSK3β (p-GSK3β)。结论:这些发现为JWQZG在NAFLD中的治疗潜力及其对胰岛素信号通路的调节提供了强有力的证据。此外,该研究为从中药制剂中发现抗nafld化合物提供了有价值的见解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Mechanistic evaluation of Jiu Wei Qing Zhi Gao in non-alcoholic fatty liver disease: insights from network Pharmacology and experimental validation.

Context: Jiu Wei Qing Zhi Gao (JWQZG), a traditional Chinese medicine (TCM) formulation, is widely utilized in China for managing non-alcoholic fatty liver disease (NAFLD).

Objective: This study aimed to elucidate the therapeutic mechanisms of JWQZG in the management of NAFLD.

Materials and methods: Network pharmacology was employed to predict the potential mechanisms of JWQZG in NAFLD management. In vivo experiments were conducted using C57BL/6J mice fed a high-fat diet (HFD) for 16 weeks, followed by treatment with JWQZG at three dosages (1.85, 3.7, and 7.4 g/kg/day) or metformin (150 mg/kg/day) for 8 weeks. In vitro studies utilized HepG2 cells exposed to 0.5 mM palmitic acid (PA) for 24 h to establish an NAFLD model, followed by exposure to JWQZG-containing serum at three concentrations for an additional 24 h. Western blot analysis was used to analyze the expression levels of key signaling pathway components.

Results: Results of network pharmacology analysis identified the insulin signaling pathway as a potential mediator of the protective effects of JWQZG in NAFLD. Treatment with JWQZG markedly reduced hepatic steatosis and improved insulin resistance. This was accompanied by enhanced expression of key components in the insulin signaling pathway, including insulin receptor substrate 1 (IRS1), phosphorylated PI3K (p-PI3K), phosphorylated AKT (p-AKT), and phosphorylated GSK3β (p-GSK3β), compared to the NAFLD model group.

Conclusions: These findings provide robust evidence supporting the therapeutic potential of JWQZG in NAFLD and its modulation of the insulin signaling pathway. Furthermore, the study offers valuable insights for the discovery of anti-NAFLD compounds derived from TCM formulations.

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来源期刊
Hereditas
Hereditas Biochemistry, Genetics and Molecular Biology-Genetics
CiteScore
3.80
自引率
3.70%
发文量
0
期刊介绍: For almost a century, Hereditas has published original cutting-edge research and reviews. As the Official journal of the Mendelian Society of Lund, the journal welcomes research from across all areas of genetics and genomics. Topics of interest include human and medical genetics, animal and plant genetics, microbial genetics, agriculture and bioinformatics.
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