lncRNA-Encoded Small Peptide Promotes Viral Infection.

The small open reading frames (ORFs) embedded in lncRNA have been identified to encode biologically functional peptides in many species. However, the function of lncRNA-encoded small peptides in the plant antiviral response remains unclear. In this study, an lncRNA Talnc54748 was identified, which encodes the small peptide ORF4. Transgenic Nicotiana benthamiana overexpressing ORF4 could enhance wheat yellow mosaic virus (WYMV) infection. RNA-sequencing analysis revealed that many genes in plant hormone signalling and MAPK signalling pathways were reduced in ORF4-overexpressing lines. The function of SAUR21 and MKK2, involved in plant hormone signalling and MAPK signalling pathways, respectively, was investigated by virus-induced gene silencing assay. Silencing SAUR21 or MKK2 in wheat and N. benthamiana enhanced WYMV infection. Transgenic overexpression of ORF4 not only enhanced the accumulation of tobacco mosaic virus (TMV) and tomato mosaic virus (ToMV), but also promoted Phytophthora capsici or Pseudomonas syringae pv. tomato DC3000 infection. Our results demonstrated that an lncRNA-encoded small peptide plays an important role in plant immunity and provides new insights into the roles of this lncRNA-encoded small peptide in the plant antiviral response.