产甲烷古菌和“Methanoperedens Candidatus Methanoperedens”mcrA基因的群体特异性定量分析。

IF 2.1 4区环境科学与生态学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Microbes and Environments Pub Date : 2025-01-01 DOI:10.1264/jsme2.ME24097

Takeshi Watanabe, Atsuya Endo, Rio Hamada, Rina Shinjo, Susumu Asakawa

{"title":"产甲烷古菌和“Methanoperedens Candidatus Methanoperedens”mcrA基因的群体特异性定量分析。","authors":"Takeshi Watanabe, Atsuya Endo, Rio Hamada, Rina Shinjo, Susumu Asakawa","doi":"10.1264/jsme2.ME24097","DOIUrl":null,"url":null,"abstract":"Digital PCR is a technique that quantifies target genes based on the absence or presence of the targets in PCR amplicons. The present study exami-ned group-specific probes for the quantification of mcrA genes in six methanogenic archaeal groups and \"Candidatus Methanoperedens\" by digital PCR with the universal primers ML-f and ML-r. A digital PCR ana-lysis of paddy field soil detected all the targets, with the dominant and minor groups being Methanomicrobiales and Methanobrevibacter spp., respectively (107 and 104 copies [g dry soil]-1). This method has the potential to reveal the dynamics of specific methanogenic archaeal groups in the environment.","PeriodicalId":18482,"journal":{"name":"Microbes and Environments","volume":"40 2","pages":""},"PeriodicalIF":2.1000,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Group-specific Quantification of mcrA genes of Methanogenic Archaea and \\\"Candidatus Methanoperedens\\\" by Digital PCR.\",\"authors\":\"Takeshi Watanabe, Atsuya Endo, Rio Hamada, Rina Shinjo, Susumu Asakawa\",\"doi\":\"10.1264/jsme2.ME24097\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Digital PCR is a technique that quantifies target genes based on the absence or presence of the targets in PCR amplicons. The present study exami-ned group-specific probes for the quantification of mcrA genes in six methanogenic archaeal groups and \\\"Candidatus Methanoperedens\\\" by digital PCR with the universal primers ML-f and ML-r. A digital PCR ana-lysis of paddy field soil detected all the targets, with the dominant and minor groups being Methanomicrobiales and Methanobrevibacter spp., respectively (107 and 104 copies [g dry soil]-1). This method has the potential to reveal the dynamics of specific methanogenic archaeal groups in the environment.\",\"PeriodicalId\":18482,\"journal\":{\"name\":\"Microbes and Environments\",\"volume\":\"40 2\",\"pages\":\"\"},\"PeriodicalIF\":2.1000,\"publicationDate\":\"2025-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Microbes and Environments\",\"FirstCategoryId\":\"93\",\"ListUrlMain\":\"https://doi.org/10.1264/jsme2.ME24097\",\"RegionNum\":4,\"RegionCategory\":\"环境科学与生态学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microbes and Environments","FirstCategoryId":"93","ListUrlMain":"https://doi.org/10.1264/jsme2.ME24097","RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}

引用次数: 0

摘要

数字PCR是一种基于PCR扩增子中目标基因的缺失或存在来量化目标基因的技术。本研究以通用引物ML-f和ML-r为引物，对6个产甲烷古菌群和“Candidatus Methanoperedens”的mcrA基因进行了群体特异性PCR检测。对水田土壤进行数字PCR分析，检测到所有目标菌，优势群和次要群分别为Methanomicrobiales和Methanobrevibacter spp.（107和104拷贝[g干土]-1）。这种方法有可能揭示环境中特定产甲烷古菌群的动态。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Group-specific Quantification of mcrA genes of Methanogenic Archaea and "Candidatus Methanoperedens" by Digital PCR.

Digital PCR is a technique that quantifies target genes based on the absence or presence of the targets in PCR amplicons. The present study exami-ned group-specific probes for the quantification of mcrA genes in six methanogenic archaeal groups and "Candidatus Methanoperedens" by digital PCR with the universal primers ML-f and ML-r. A digital PCR ana-lysis of paddy field soil detected all the targets, with the dominant and minor groups being Methanomicrobiales and Methanobrevibacter spp., respectively (10⁷ and 10⁴ copies [g dry soil]^-1). This method has the potential to reveal the dynamics of specific methanogenic archaeal groups in the environment.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Microbes and Environments 生物-生物工程与应用微生物

CiteScore

4.10

自引率

13.60%

发文量

审稿时长

3 months

期刊介绍： Microbial ecology in natural and engineered environments; Microbial degradation of xenobiotic compounds; Microbial processes in biogeochemical cycles; Microbial interactions and signaling with animals and plants; Interactions among microorganisms; Microorganisms related to public health; Phylogenetic and functional diversity of microbial communities; Genomics, metagenomics, and bioinformatics for microbiology; Application of microorganisms to agriculture, fishery, and industry; Molecular biology and biochemistry related to environmental microbiology; Methodology in general and environmental microbiology; Interdisciplinary research areas for microbial ecology (e.g., Astrobiology, and Origins of Life); Taxonomic description of novel microorganisms with ecological perspective; Physiology and metabolisms of microorganisms; Evolution of genes and microorganisms; Genome report of microorganisms with ecological perspective.