Ehsan Javanmard, Hanieh Mohammad Rahimi, Ali Taghipour, Sara Nemati, Mehdi Mohebali, Mostafa Rezaeian, Ali Badrifar, Elham Kazemirad, Hamed Mirjalali
{"title":"BEB6基因型在伊朗洛雷斯坦省野猪中高发：人畜共患病传播的潜在来源","authors":"Ehsan Javanmard, Hanieh Mohammad Rahimi, Ali Taghipour, Sara Nemati, Mehdi Mohebali, Mostafa Rezaeian, Ali Badrifar, Elham Kazemirad, Hamed Mirjalali","doi":"10.18502/ijpa.v20i1.18113","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>We aimed to investigate the prevalence of <i>Enterocytozoon bieneusi</i> and the circulating genotypes in wild boar in the western regions of Iran.</p><p><strong>Methods: </strong>Fifty-two fecal samples were collected from wild boars in Lorestan province, Iran. After DNA extraction, the presence of <i>E. bieneusi</i> was evaluated by real-time PCR. A nested PCR targeting the internal transcribed region (ITS) was employed to characterize genotypes. The PCR products were sequenced, and the genetic diversity and relationships among the genotypes were identified using MEGA X and DnaSp (V5) software.</p><p><strong>Results: </strong>Sixteen (30%) samples were positive for <i>E. bieneusi</i> using real-time PCR, and 11 (21%) were positive for nested PCR, which was sequenced. All 11 positive samples were identified as the BEB6 genotype (Group 2). The haplotype diversity was noted to be 0.182, and the nucleotide diversity, calculated using DnaSp, was 0. 00085.</p><p><strong>Conclusion: </strong>The findings highlight the high prevalence of the genotype BEB6 in wild boars. The presence of this genotype suggests the circulation of <i>E. bieneusi</i> between domesticated animals and wild boars in Iran.</p>","PeriodicalId":14669,"journal":{"name":"Iranian Journal of Parasitology","volume":"20 1","pages":"130-139"},"PeriodicalIF":0.9000,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11978206/pdf/","citationCount":"0","resultStr":"{\"title\":\"High Prevalence of <i>Enterocytozoon bieneusi</i> Genotype BEB6 in Wild Boars in Lorestan Province, Iran: Potential Source of Zoonotic Transmission.\",\"authors\":\"Ehsan Javanmard, Hanieh Mohammad Rahimi, Ali Taghipour, Sara Nemati, Mehdi Mohebali, Mostafa Rezaeian, Ali Badrifar, Elham Kazemirad, Hamed Mirjalali\",\"doi\":\"10.18502/ijpa.v20i1.18113\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>We aimed to investigate the prevalence of <i>Enterocytozoon bieneusi</i> and the circulating genotypes in wild boar in the western regions of Iran.</p><p><strong>Methods: </strong>Fifty-two fecal samples were collected from wild boars in Lorestan province, Iran. After DNA extraction, the presence of <i>E. bieneusi</i> was evaluated by real-time PCR. A nested PCR targeting the internal transcribed region (ITS) was employed to characterize genotypes. The PCR products were sequenced, and the genetic diversity and relationships among the genotypes were identified using MEGA X and DnaSp (V5) software.</p><p><strong>Results: </strong>Sixteen (30%) samples were positive for <i>E. bieneusi</i> using real-time PCR, and 11 (21%) were positive for nested PCR, which was sequenced. All 11 positive samples were identified as the BEB6 genotype (Group 2). The haplotype diversity was noted to be 0.182, and the nucleotide diversity, calculated using DnaSp, was 0. 00085.</p><p><strong>Conclusion: </strong>The findings highlight the high prevalence of the genotype BEB6 in wild boars. The presence of this genotype suggests the circulation of <i>E. bieneusi</i> between domesticated animals and wild boars in Iran.</p>\",\"PeriodicalId\":14669,\"journal\":{\"name\":\"Iranian Journal of Parasitology\",\"volume\":\"20 1\",\"pages\":\"130-139\"},\"PeriodicalIF\":0.9000,\"publicationDate\":\"2025-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11978206/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Iranian Journal of Parasitology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.18502/ijpa.v20i1.18113\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"PARASITOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Iranian Journal of Parasitology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.18502/ijpa.v20i1.18113","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"PARASITOLOGY","Score":null,"Total":0}
High Prevalence of Enterocytozoon bieneusi Genotype BEB6 in Wild Boars in Lorestan Province, Iran: Potential Source of Zoonotic Transmission.
Background: We aimed to investigate the prevalence of Enterocytozoon bieneusi and the circulating genotypes in wild boar in the western regions of Iran.
Methods: Fifty-two fecal samples were collected from wild boars in Lorestan province, Iran. After DNA extraction, the presence of E. bieneusi was evaluated by real-time PCR. A nested PCR targeting the internal transcribed region (ITS) was employed to characterize genotypes. The PCR products were sequenced, and the genetic diversity and relationships among the genotypes were identified using MEGA X and DnaSp (V5) software.
Results: Sixteen (30%) samples were positive for E. bieneusi using real-time PCR, and 11 (21%) were positive for nested PCR, which was sequenced. All 11 positive samples were identified as the BEB6 genotype (Group 2). The haplotype diversity was noted to be 0.182, and the nucleotide diversity, calculated using DnaSp, was 0. 00085.
Conclusion: The findings highlight the high prevalence of the genotype BEB6 in wild boars. The presence of this genotype suggests the circulation of E. bieneusi between domesticated animals and wild boars in Iran.
期刊介绍：
Iranian Journal of Parasitology (IJP) is the official publication of Iranian Society of Parasitology (ISP) launched in 2006. The society was inaugurated in 1994 and pursues the improvement of the knowledge on the parasites and parasitic diseases, exchange of scientific knowledge with foreign societies, publicity activities, and consultation on the parasitic diseases, and intimate relationship among society members.
The main aims of the Journal are: contribution to the field of Parasitology, including all aspects of parasites and parasitic diseases (medical and veterinary) and related fields such as Entomology which may be submitted by scientists from Iran and all over the world.
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