土耳其PLWH多瘤病毒基因型的分子流行病学研究。

IF 1.9 4区 医学 Q3 NEUROSCIENCES
Journal of NeuroVirology Pub Date : 2025-06-01 Epub Date: 2025-05-12 DOI:10.1007/s13365-025-01262-x
Okan Aydoğan, Ezgi Gözün Şaylan, Bilgül Mete, Ahmet Çağkan İnkaya, Özlem Güven
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引用次数: 0

摘要

背景:JC多瘤病毒(JCPyV)是一种全球流行的人类多瘤病毒,具有终身潜伏期,主要发生在肾组织。尽管JCPyV具有全球重要性,但其分子流行病学数据仍然有限。目的:本研究旨在调查土耳其HIV感染者(PLWH)尿液和血浆样本中JCPyV的患病率、基因型分布和遗传变异。此外,我们探讨了JCPyV的存在、免疫学参数和人口统计学因素之间的关系,提供了该人群中JCPyV的第一个分子流行病学报告。对107名PLWH患者和77名健康对照者进行了一项前瞻性、多中心、横断面研究。采用qPCR检测并定量JCPyV DNA,并进行VP1基因测序,确定病毒基因型。采用Clustal Omega和Neighbour-Joining法进行系统发育分析,自举值为1000。结果:46%的PLWH和18.18%的健康人检出JCPyV病毒,病毒频率与免疫缺陷严重程度无显著相关性(p < 0.05)。基因型最多(37.5%),其次是基因型(31.25%)和基因型(31.25%),与欧洲流行病学数据一致。未检测到基因III型。未发现与PML或免疫逃避相关的VP1突变。然而,在74、92、116、127和133位上观察到氨基酸取代,值得进一步研究。结论:本研究首次提供了土耳其PLWH中JCPyV的分子流行病学分析,显示了与欧洲数据一致的基因型分布。虽然没有检测到明显的pml相关VP1突变,但替换的鉴定强调了继续进行分子监测的必要性。了解JCPyV基因型动力学和免疫逃避策略对于开发靶向治疗至关重要,包括针对高危人群的基于vp1的疫苗和单克隆抗体治疗。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Molecular epidemiology of JC polyomavirus genotypes in PLWH from Turkey.

Background: JC polyomavirus (JCPyV) is a globally prevalent human polyomavirus that establishes lifelong latency, primarily in renal tissue. Despite its global importance, molecular epidemiological data on JCPyV still remain limited.

Objectives: This study aimed to investigate the prevalence, genotype distribution, and genetic variations of JCPyV in urine and plasma samples from people living with HIV (PLWH) in Turkey. Additionally, we explored the correlation between JCPyV presence, immunological parameters, and demographic factors, providing the first molecular epidemiological report of JCPyV in this population. A prospective, multicentre, cross-sectional study was conducted on 107 PLWH and 77 healthy controls. JCPyV DNA was detected and quantified using qPCR, and VP1 gene sequencing was performed to determine viral genotypes. Phylogenetic analysis was conducted using Clustal Omega and the Neighbour-Joining method with a bootstrap value of 1000.

Results: JCPyV viruria was detected in 46% of PLWH and 18.18% of healthy individuals, with no significant association between viruria frequency and immunodeficiency severity (p > 0.05). Genotype IV was the most prevalent (37.5%), followed by Genotype I (31.25%) and Genotype II (31.25%), aligning with European epidemiological data. No Genotype III was detected. No VP1 mutations associated with PML or immune evasion were identified. However, amino acid substitutions were observed at positions 74, 92, 116, 127, and 133, warranting further investigation.

Conclusion: This study provides the first molecular epidemiological analysis of JCPyV in PLWH from Turkey, demonstrating a genotype distribution consistent with European data. While no significant PML-associated VP1 mutations were detected, the identification of substitutions underscores the need for continued molecular surveillance. Understanding JCPyV genotype dynamics and immune evasion strategies is crucial for developing targeted therapeutics, including VP1-based vaccines and monoclonal antibody treatments for high-risk populations.

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来源期刊
Journal of NeuroVirology
Journal of NeuroVirology 医学-病毒学
CiteScore
6.60
自引率
3.10%
发文量
77
审稿时长
6-12 weeks
期刊介绍: The Journal of NeuroVirology (JNV) provides a unique platform for the publication of high-quality basic science and clinical studies on the molecular biology and pathogenesis of viral infections of the nervous system, and for reporting on the development of novel therapeutic strategies using neurotropic viral vectors. The Journal also emphasizes publication of non-viral infections that affect the central nervous system. The Journal publishes original research articles, reviews, case reports, coverage of various scientific meetings, along with supplements and special issues on selected subjects. The Journal is currently accepting submissions of original work from the following basic and clinical research areas: Aging & Neurodegeneration, Apoptosis, CNS Signal Transduction, Emerging CNS Infections, Molecular Virology, Neural-Immune Interaction, Novel Diagnostics, Novel Therapeutics, Stem Cell Biology, Transmissable Encephalopathies/Prion, Vaccine Development, Viral Genomics, Viral Neurooncology, Viral Neurochemistry, Viral Neuroimmunology, Viral Neuropharmacology.
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