睾丸双侧生殖细胞瘤：肿瘤干细胞与基因起源的生物学和临床意义。

IF 5.1 2区生物学 Q2 CELL BIOLOGY

Cells Pub Date : 2025-04-30 DOI:10.3390/cells14090658

Jamaal C Jackson, Darren Sanchez, Aron Y Joon, Marcos R Estecio, Andrew C Johns, Amishi Y Shah, Matthew Campbell, John F Ward, Louis L Pisters, Charles C Guo, Miao Zhang, Niki M Zacharias, Shi-Ming Tu

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引用次数: 0

摘要

睾丸生殖细胞肿瘤（gct）为研究肿瘤的细胞与遗传起源提供了理想的肿瘤模型。在这项单一机构研究中，我们评估了38例双侧GCT患者，包括同时发生的肿瘤（同步）和在不同时间发生的肿瘤（异时）。对其中的9名患者，从右侧和左侧GCT中分离DNA，使用全外显子组测序（WES）和减少亚硫酸氢盐代表性测序（RRBS）来确定组织之间的基因组和表观遗传差异。我们发现精原细胞瘤和非精原细胞瘤在分子上是不同的，基于DNA甲基化，而不是由于同步或异时性疾病。此外，我们没有观察到在同步或异时性疾病中右侧和左侧GCT基因突变的保守性。我们的数据表明双侧GCT的细胞起源。

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Bilateral Germ Cell Tumor of the Testis: Biological and Clinical Implications for a Stem Versus Genetic Origin of Cancers.

Germ cell tumors of the testis (GCTs) provide an ideal tumor model to investigate the cellular versus genetic origin of cancers. In this single institutional study, we evaluated 38 patients with bilateral GCT, including tumors that occurred simultaneously (synchronous) and those occurring at different times (metachronous). For nine of these patients, DNA was isolated from the right and left GCT to determine the genomic and epigenetic differences between tissues using whole-exome sequencing (WES) and reduced representation bisulfite sequencing (RRBS). We found that seminomas and non-seminomas are molecularly distinct based on DNA methylation and not due to synchronous or metachronous disease. In addition, we did not observe conservation of genetic mutations in right and left GCT in either synchronous or metachronous disease. Our data suggest a cellular origin for bilateral GCT.

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来源期刊

Cells Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)

CiteScore

9.90

自引率

5.00%

发文量

3472

审稿时长

16 days

期刊介绍： Cells (ISSN 2073-4409) is an international, peer-reviewed open access journal which provides an advanced forum for studies related to cell biology, molecular biology and biophysics. It publishes reviews, research articles, communications and technical notes. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. Full experimental and/or methodical details must be provided.