毛茛(Ranunculus ternatus)的机制。基于网络药理学及实验验证的食管鳞状细胞癌防治研究

IF 1.6 4区 医学 Q4 BIOCHEMICAL RESEARCH METHODS
Michen Deng, Yanhong Deng, Zugui Tang, Wenqiang Nie, Yi Lu, Xicheng Wang, Zhe Wang, Yi Kong
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引用次数: 0

摘要

背景:食管鳞状细胞癌(ESCC)仍然是一个重大的全球健康挑战,强调迫切需要开发创新的治疗方法。毛茛。作为一种传统的中草药,已显示出潜在的抗癌特性,但其抗ESCC的药理机制尚不清楚。本研究旨在阐明毛茛乙酸乙酯提取物的分子机制。通过网络药理学分析和实验验证相结合的方法研究了RTE对ESCC的抑制作用。方法:利用公共数据库,通过网络药理学方法鉴定RTE和ESCC的潜在靶点。通过构建蛋白-蛋白相互作用(PPI)网络来识别关键靶点,然后通过基因本体(GO)和京都基因与基因组百科全书(KEGG)途径富集分析来探索生物过程和信号通路。通过体外实验验证了预测的机制,包括细胞增殖分析和细胞免疫印迹法。结果:网络药理学分析确定了274个潜在靶点,其中14个关键基因与RTE的治疗效果有关。氧化石墨烯分析显示,氧化石墨烯参与炎症反应和凋亡信号通路。KEGG通路分析强调MAPK、Relaxin和PI3K/Akt信号通路是关键机制。体外实验表明,RTE通过调控MAPK/ERK和PI3K/Akt通路,显著抑制EC-109和TE-13细胞的增殖。结论:本研究为RTE抗ESCC的分子机制提供了全面的认识。这些发现强调了RTE作为ESCC治疗的天然化合物的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The Mechanism of Ranunculus ternatus Thunb. against Esophageal Squamous Cell Carcinoma Based on Network Pharmacology and Experimental Verification.

Background: Esophageal Squamous Cell Carcinoma (ESCC) remains a significant global health challenge, underscoring the urgent need for the development of innovative therapeutic approaches. Ranunculus ternatus Thunb., a traditional herbal medicine, has shown potential anticancer properties, but its pharmacological mechanisms against ESCC remain poorly understood. This study aimed to elucidate the molecular mechanisms of the ethyl acetate extract of Ranunculus ternatus Thunb. (RTE) against ESCC by integrating network pharmacology analysis with experimental validation.

Methods: Potential targets of RTE and ESCC were identified through network pharmacology using public databases. A Protein-Protein Interaction (PPI) network was constructed to identify key targets, followed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses to explore biological processes and signaling pathways. The predicted mechanisms were experimentally validated using in vitro assays, including cell proliferation analysis and western blot assay in ESCC cell lines.

Results: Network pharmacology analysis identified 274 potential targets, with 14 key genes implicated in the therapeutic effects of RTE. GO analysis revealed significant involvement in the inflammatory response and apoptotic signaling pathways. KEGG pathway analysis highlighted the MAPK, Relaxin, and PI3K/Akt signaling pathways as critical mechanisms. In vitro experiments demonstrated that RTE significantly inhibited the proliferation of EC-109 and TE-13 cells by modulating the MAPK/ERK and PI3K/Akt pathways.

Conclusion: This study provides a comprehensive understanding of the molecular mechanisms underlying the anticancer effects of RTE against ESCC. These findings underscore the potential of RTE as a promising natural compound for ESCC treatment.

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来源期刊
CiteScore
3.10
自引率
5.60%
发文量
327
审稿时长
7.5 months
期刊介绍: Combinatorial Chemistry & High Throughput Screening (CCHTS) publishes full length original research articles and reviews/mini-reviews dealing with various topics related to chemical biology (High Throughput Screening, Combinatorial Chemistry, Chemoinformatics, Laboratory Automation and Compound management) in advancing drug discovery research. Original research articles and reviews in the following areas are of special interest to the readers of this journal: Target identification and validation Assay design, development, miniaturization and comparison High throughput/high content/in silico screening and associated technologies Label-free detection technologies and applications Stem cell technologies Biomarkers ADMET/PK/PD methodologies and screening Probe discovery and development, hit to lead optimization Combinatorial chemistry (e.g. small molecules, peptide, nucleic acid or phage display libraries) Chemical library design and chemical diversity Chemo/bio-informatics, data mining Compound management Pharmacognosy Natural Products Research (Chemistry, Biology and Pharmacology of Natural Products) Natural Product Analytical Studies Bipharmaceutical studies of Natural products Drug repurposing Data management and statistical analysis Laboratory automation, robotics, microfluidics, signal detection technologies Current & Future Institutional Research Profile Technology transfer, legal and licensing issues Patents.
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