LncRNA SNHG1/miR-320b/CTNNB1 axis regulating the collective migration of fibroblasts in the formation of keloid.

Background: To explore the regulatory molecular mechanism of long non-coding RNA (lncRNA) small nucleolar RNA host gene 1 (SNHG1) expression on keloid formation.

Methods: The expression differences of SNHG1, miR-320b, and Catenin Beta 1 (CTNNB1) in keloid tissue and normal skin tissue of patients with keloid were detected. Normal cultured human fibroblasts were used as the Blank group (Blank) and then transfected with si-SNHG1 to silence SNHG1 expression. MTT assay, Transwell chamber assay, RT-qPCR, and Western blot (WB) were used. SNHG1 and miR-320b, as well as miR-320b and CTNNB1, were found to be targeted using the dual luciferase reporter gene (DLRG) strategy.

Results: As against normal skin tissue, SNHG1 and CTNNB1 were increased, while miR-320b was decreased in keloid tissue (P < 0.05). As against the Blank, there was a drop in the number of transferring and attacking cells, a decrease in the proliferative activity, an increase in the expression of miR-320b, a decrease in CTNNB1, and the relative expression (RE) of Pro-Collagen I, Cyclin D1, VEGF, α-smooth muscle actin (α-SMA), matrix metallopeptidase-2 (MMP-2), and MMP-9 was decreased in the si-SNHG1 group (AG) (P < 0.05).

Conclusion: SNHG1 could target and regulate miR-320b, and miR-320b could target and regulate CTNNB1. Fibroblast transfer, attack, and multiplication may all be prevented by reducing SNHG1 expression.