Honey Bees Can Use Sequence Learning to Predict Rewards from a Prior Unrewarded Visual Stimulus.

Learning to anticipate upcoming events can increase fitness by allowing animals to choose the best course of action, and many species can learn sequences of events and anticipate rewards. To date, most studies have focused on sequences over short time scales such as a few seconds. Whereas events separated by a few seconds are easily learned, events separated by longer delays are typically more difficult to learn. Here, we show that honey bees (Apis mellifera) can learn a sequence of two visually distinct food sources alternating in profitability every few minutes. Bees were challenged to learn that the rewarded pattern was the one that was non-rewarded on the prior visit. We show that bees can predict and choose the feeder that will be rewarding upon their next approach more frequently than predicted by chance, and they improve with experience, with 64% correct choices made in the second half of their visit sequence (N = 320 visits by 20 different bees). These results increase our understanding of honey bee visual sequential learning and further demonstrate the flexibility of foragers' learning strategies.