Gabriel Montenegro de Campos, Thalita Cristina de Mello Costa, Anielly Sarana da Silva, Lara Okuyama Afonso Costa, Roberta Maraninchi Silveira, Ian Nunes Valença, Felipe Santos de Carvalho, Luiz Guilherme Darrigo Júnior, Rodrigo Haddad, Ana Carolina de Jesus Vieira, Camila Campos Mesquita, Patrícia da Silva Laurindo, Renato Guerino Cunha, Luiz Carlos Júnior Alcântara, Simone Kashima, Dimas Tadeu Covas, Belinda Pinto Simões, Sandra Coccuzzo Sampaio, Maria Carolina Elias, Marta Giovanetti, Dennis Maletich Junqueira, Svetoslav Nanev Slavov
{"title":"巴西接受同种异体造血干细胞移植患者中共生病毒的分子特征和动力学。","authors":"Gabriel Montenegro de Campos, Thalita Cristina de Mello Costa, Anielly Sarana da Silva, Lara Okuyama Afonso Costa, Roberta Maraninchi Silveira, Ian Nunes Valença, Felipe Santos de Carvalho, Luiz Guilherme Darrigo Júnior, Rodrigo Haddad, Ana Carolina de Jesus Vieira, Camila Campos Mesquita, Patrícia da Silva Laurindo, Renato Guerino Cunha, Luiz Carlos Júnior Alcântara, Simone Kashima, Dimas Tadeu Covas, Belinda Pinto Simões, Sandra Coccuzzo Sampaio, Maria Carolina Elias, Marta Giovanetti, Dennis Maletich Junqueira, Svetoslav Nanev Slavov","doi":"10.1111/tid.70037","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Commensal viruses are typically well-tolerated by healthy individuals, but their behavior in immunocompromised patients is not fully understood.</p><p><strong>Methods: </strong>This study investigated the prevalence, molecular dynamics, and circulating genotypes of human pegivirus-1 (HPgV-1) and three types of torque teno viruses (TTV-3, -16, and -22) in Brazilian patients undergoing allogeneic hematopoietic stem cell transplantation (HSCT). Plasma samples from 20 patients were collected at three time points: pretransplantation (D+0), 30 days post-transplantation (D+30), and 100 days post-transplantation (D+100). We conducted longitudinal screening for HPgV-1 and three TTV variants using real-time PCR, comparing cycle threshold values across these intervals.</p><p><strong>Results: </strong>The overall HPgV-1 RNA prevalence at all collection points was 45% (n = 9/20), with all infected individuals carrying genotype 2. HPgV-1 infections exhibited stable, closely related strains over time, though virus evolution was highly individualized. The overall prevalence of TTV types was 5% for TTV-3, 30% for TTV-16, and 60% for TTV-22. Notably, all commensal viruses showed a decrease in cycle threshold at D+100, indicating a possible increase in viral load.</p><p><strong>Conclusion: </strong>These findings underscore the importance of commensal viruses in the context of HSCT and suggest their potential role as biomarkers for immune suppression and transplantation outcomes. Further research is warranted to elucidate their implications.</p>","PeriodicalId":23318,"journal":{"name":"Transplant Infectious Disease","volume":" ","pages":"e70037"},"PeriodicalIF":2.6000,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Molecular Profile and Dynamics of Commensal Viruses in Brazilian Patients Who Underwent Allogeneic Hematopoietic Stem Cell Transplantation.\",\"authors\":\"Gabriel Montenegro de Campos, Thalita Cristina de Mello Costa, Anielly Sarana da Silva, Lara Okuyama Afonso Costa, Roberta Maraninchi Silveira, Ian Nunes Valença, Felipe Santos de Carvalho, Luiz Guilherme Darrigo Júnior, Rodrigo Haddad, Ana Carolina de Jesus Vieira, Camila Campos Mesquita, Patrícia da Silva Laurindo, Renato Guerino Cunha, Luiz Carlos Júnior Alcântara, Simone Kashima, Dimas Tadeu Covas, Belinda Pinto Simões, Sandra Coccuzzo Sampaio, Maria Carolina Elias, Marta Giovanetti, Dennis Maletich Junqueira, Svetoslav Nanev Slavov\",\"doi\":\"10.1111/tid.70037\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Commensal viruses are typically well-tolerated by healthy individuals, but their behavior in immunocompromised patients is not fully understood.</p><p><strong>Methods: </strong>This study investigated the prevalence, molecular dynamics, and circulating genotypes of human pegivirus-1 (HPgV-1) and three types of torque teno viruses (TTV-3, -16, and -22) in Brazilian patients undergoing allogeneic hematopoietic stem cell transplantation (HSCT). Plasma samples from 20 patients were collected at three time points: pretransplantation (D+0), 30 days post-transplantation (D+30), and 100 days post-transplantation (D+100). We conducted longitudinal screening for HPgV-1 and three TTV variants using real-time PCR, comparing cycle threshold values across these intervals.</p><p><strong>Results: </strong>The overall HPgV-1 RNA prevalence at all collection points was 45% (n = 9/20), with all infected individuals carrying genotype 2. HPgV-1 infections exhibited stable, closely related strains over time, though virus evolution was highly individualized. The overall prevalence of TTV types was 5% for TTV-3, 30% for TTV-16, and 60% for TTV-22. Notably, all commensal viruses showed a decrease in cycle threshold at D+100, indicating a possible increase in viral load.</p><p><strong>Conclusion: </strong>These findings underscore the importance of commensal viruses in the context of HSCT and suggest their potential role as biomarkers for immune suppression and transplantation outcomes. 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Molecular Profile and Dynamics of Commensal Viruses in Brazilian Patients Who Underwent Allogeneic Hematopoietic Stem Cell Transplantation.
Background: Commensal viruses are typically well-tolerated by healthy individuals, but their behavior in immunocompromised patients is not fully understood.
Methods: This study investigated the prevalence, molecular dynamics, and circulating genotypes of human pegivirus-1 (HPgV-1) and three types of torque teno viruses (TTV-3, -16, and -22) in Brazilian patients undergoing allogeneic hematopoietic stem cell transplantation (HSCT). Plasma samples from 20 patients were collected at three time points: pretransplantation (D+0), 30 days post-transplantation (D+30), and 100 days post-transplantation (D+100). We conducted longitudinal screening for HPgV-1 and three TTV variants using real-time PCR, comparing cycle threshold values across these intervals.
Results: The overall HPgV-1 RNA prevalence at all collection points was 45% (n = 9/20), with all infected individuals carrying genotype 2. HPgV-1 infections exhibited stable, closely related strains over time, though virus evolution was highly individualized. The overall prevalence of TTV types was 5% for TTV-3, 30% for TTV-16, and 60% for TTV-22. Notably, all commensal viruses showed a decrease in cycle threshold at D+100, indicating a possible increase in viral load.
Conclusion: These findings underscore the importance of commensal viruses in the context of HSCT and suggest their potential role as biomarkers for immune suppression and transplantation outcomes. Further research is warranted to elucidate their implications.
期刊介绍：
Transplant Infectious Disease has been established as a forum for presenting the most current information on the prevention and treatment of infection complicating organ and bone marrow transplantation. The point of view of the journal is that infection and allograft rejection (or graft-versus-host disease) are closely intertwined, and that advances in one area will have immediate consequences on the other. The interaction of the transplant recipient with potential microbial invaders, the impact of immunosuppressive strategies on this interaction, and the effects of cytokines, growth factors, and chemokines liberated during the course of infections, rejection, or graft-versus-host disease are central to the interests and mission of this journal.
Transplant Infectious Disease is aimed at disseminating the latest information relevant to the infectious disease complications of transplantation to clinicians and scientists involved in bone marrow, kidney, liver, heart, lung, intestinal, and pancreatic transplantation. The infectious disease consequences and concerns regarding innovative transplant strategies, from novel immunosuppressive agents to xenotransplantation, are very much a concern of this journal. In addition, this journal feels a particular responsibility to inform primary care practitioners in the community, who increasingly are sharing the responsibility for the care of these patients, of the special considerations regarding the prevention and treatment of infection in transplant recipients. As exemplified by the international editorial board, articles are sought throughout the world that address both general issues and those of a more restricted geographic import.
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