代谢组学和网络药理学分析揭示艾灸治疗膝骨关节炎的机制。

IF 1.6 4区 医学 Q4 BIOCHEMICAL RESEARCH METHODS
Yaqiong Su, Minfeng Fang, Ziyao Qiao, Na Zheng, Yun Yang, Jingjing Li, Weijian Zhao, Yaning Zhang, Hong Zhang, Ye Li, Chunliu Wang
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引用次数: 0

摘要

背景:本研究旨在结合骨关节炎代谢组学和网络药理学,探讨艾灸对膝关节的作用机制。方法:采用关节内注射木瓜蛋白酶建立大鼠膝关节骨性关节炎模型。以膝关节肿胀程度、病理进展、活动能力丧失等指标评价艾灸对KOA大鼠的疗效。在此基础上,通过代谢组学分析艾灸缓解膝关节骨性关节炎的代谢机制。结果:艾灸能明显减轻KOA大鼠膝关节肿胀和炎症。代谢组学分析结果发现16种代谢物和9种代谢途径与艾灸的作用机制有关。根据网络药理学,共获得KOA疾病靶点3186个,药物靶点158个,交叉靶点89个。关键靶点包括MAPK-3、AKT-1、RELA、MAPK-8、MAPK-14等。发现艾灸治疗膝关节骨关节炎的机制涉及谷氨酸、天冬氨酸和谷氨酸代谢、半胱氨酸和蛋氨酸代谢、精氨酸和脯氨酸代谢等信号通路。结论:艾灸治疗膝关节骨性关节炎的机制可能涉及丙氨酸、天冬氨酸、谷氨酸代谢、半胱氨酸、甲硫氨酸代谢、精氨酸、脯氨酸代谢、氨基tRNA生物合成以及以MAPK-3、AKT-1、RELA、MAPK-8、MAPK-14为核心靶点的d -谷氨酰胺和d -谷氨酸代谢信号通路。更精确的机制需要通过进一步系统的分子生物学实验来验证。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Metabolomics and Network Pharmacology Analyses Reveal the Mechanism of Moxibustion in Knee Osteoarthritis.

Background: This study aimed to explore the mechanism of moxibustion in the knee by combining osteoarthritis metabolomics and network pharmacology.

Methods: A rat knee osteoarthritis (KOA) model was established by intra-articular injection of papain. The efficacy of moxibustion in KOA rats was evaluated by swelling degree, pathological progress, and mobility loss of knee joint. On this basis, the metabolic mechanism of moxibustion in relieving knee osteoarthritis was analyzed by metabolomics analysis.

Results: Moxibustion significantly reduced joint swelling and inflammation in the knee joint of KOA rats. Sixteen metabolites and nine metabolic pathways were found to be associated with the mechanism of action of moxibustion in metabolomics analysis results. According to network pharmacology, 3186 KOA disease targets, 158 drug targets, and 89 intersecting targets were obtained. The key targets included MAPK-3, AKT-1, RELA, MAPK-8, MAPK-14, etc. Signal pathways were found to be involved in mechanisms of moxibustion in knee osteoarthritis, such as alanine, aspartate, and glutamate metabolism, cysteine and methionine metabolism, and arginine and proline metabolism.

Conclusion: The mechanism of moxibustion in knee osteoarthritis may involve alanine, aspartate, and glutamate metabolism, cysteine and methionine metabolism, arginine and proline metabolism, amino tRNA biosynthesis, and D-glutamine and D-glutamate metabolism signaling pathways with MAPK-3, AKT-1, RELA, MAPK-8, and MAPK-14 as core targets. More precise mechanisms need to be verified by further systematic molecular biology experiments.

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来源期刊
CiteScore
3.10
自引率
5.60%
发文量
327
审稿时长
7.5 months
期刊介绍: Combinatorial Chemistry & High Throughput Screening (CCHTS) publishes full length original research articles and reviews/mini-reviews dealing with various topics related to chemical biology (High Throughput Screening, Combinatorial Chemistry, Chemoinformatics, Laboratory Automation and Compound management) in advancing drug discovery research. Original research articles and reviews in the following areas are of special interest to the readers of this journal: Target identification and validation Assay design, development, miniaturization and comparison High throughput/high content/in silico screening and associated technologies Label-free detection technologies and applications Stem cell technologies Biomarkers ADMET/PK/PD methodologies and screening Probe discovery and development, hit to lead optimization Combinatorial chemistry (e.g. small molecules, peptide, nucleic acid or phage display libraries) Chemical library design and chemical diversity Chemo/bio-informatics, data mining Compound management Pharmacognosy Natural Products Research (Chemistry, Biology and Pharmacology of Natural Products) Natural Product Analytical Studies Bipharmaceutical studies of Natural products Drug repurposing Data management and statistical analysis Laboratory automation, robotics, microfluidics, signal detection technologies Current & Future Institutional Research Profile Technology transfer, legal and licensing issues Patents.
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