印楝和苦楝提取物在小反刍动物慢病毒复制周期中的抗病毒活性。

IF 1.9 4区 生物学 Q3 MICROBIOLOGY
Brazilian Journal of Microbiology Pub Date : 2025-09-01 Epub Date: 2025-04-26 DOI:10.1007/s42770-025-01659-0
Ana Lídia Madeira de Sousa, Raymundo Rizaldo Pinheiro, Juscilânia Furtado Araújo, Renato Mesquita Peixoto, Ana Milena César Lima, Selene Maia de Morais, Samara Cristina Rocha Souza, Gabriel Paula Amaral, Maria Fátima da Silva Teixeira, Alice Andrioli
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引用次数: 0

摘要

本研究旨在评价印楝和苦楝乙醇提取物在小反刍动物慢病毒(SRLV)组CAEV-Cork和MVV-K1514株在羊第三眼睑(OTE)细胞复制周期内的体外抗病毒活性。将这些植物的粗提取物(CE)、乙酸乙酯(EAF)和甲醇(MF)有机组分分别在CAEV-Cork和MVV-K1514菌株在不同的OTE细胞培养中接种病毒之前、期间和之后进行了处理测试。在这些治疗之后,对最有效的结果进行病毒滴定分析。结果表明,苦楝和苦楝的EAF可使CAEV-Cork株接种后的病毒滴度分别降低316倍和1.995倍。对于MVV-K1514菌株,当与病毒接种同时施用时,印度芽孢杆菌的EAF达到了1000倍的抑制作用。这些结果表明,五味科植物提取物影响SRLV复制周期的所有阶段,显著抑制两种病毒株的活性,尽管没有完全消除病毒。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Antiviral activity of Azadirachta indica A. Juss and Melia azedarach extracts during the replication cycle of small ruminant lentiviruses.

This study aimed to evaluate the in vitro antiviral activity of ethanolic extracts from Azadirachta indica and Melia azedarach during the replication cycle of the CAEV-Cork and MVV-K1514 strains, both part of the small ruminant lentivirus (SRLV) group, in ovine third eyelid (OTE) cells. The crude extracts (CE), along with the ethyl acetate (EAF) and methanol (MF) organic fractions from these plants, were tested in treatments applied before, during, and after viral inoculation of the CAEV-Cork and MVV-K1514 strains in separate OTE cell cultures. Following these treatments, the most effective results were subjected to viral titration analysis. The findings indicate that the EAF of A. indica and M. azedarach may have reduced the viral titer of the CAEV-Cork strain by 316-fold after inoculation and by 1.995-fold when applied concomitantly with inoculation, respectively. For the MVV-K1514 strain, the EAF of A. indica achieved a 1,000-fold inhibition when applied simultaneously with viral inoculation. These results suggest that extracts from Meliaceae plants influenced all phases of the SRLV replication cycle, significantly inhibiting the activity of both viral strains, although complete viral elimination was not achieved.

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来源期刊
Brazilian Journal of Microbiology
Brazilian Journal of Microbiology 生物-微生物学
CiteScore
4.10
自引率
4.50%
发文量
216
审稿时长
1.0 months
期刊介绍: The Brazilian Journal of Microbiology is an international peer reviewed journal that covers a wide-range of research on fundamental and applied aspects of microbiology. The journal considers for publication original research articles, short communications, reviews, and letters to the editor, that may be submitted to the following sections: Biotechnology and Industrial Microbiology, Food Microbiology, Bacterial and Fungal Pathogenesis, Clinical Microbiology, Environmental Microbiology, Veterinary Microbiology, Fungal and Bacterial Physiology, Bacterial, Fungal and Virus Molecular Biology, Education in Microbiology. For more details on each section, please check out the instructions for authors. The journal is the official publication of the Brazilian Society of Microbiology and currently publishes 4 issues per year.
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