耐高温振荡藻蓝蛋白的结构与稳定性。

IF 3.5 4区生物学 Q1 Biochemistry, Genetics and Molecular Biology

FEBS Letters Pub Date : 2025-04-29 DOI:10.1002/1873-3468.70048

Stuti N. Patel, Ravi R. Sonani, Gagan D. Gupta, Niraj Kumar Singh, Chandni Upadhyaya, Bhargavi Sonavane, Seema Amin, Vinay Kumar, Datta Madamwar

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引用次数: 0

摘要

藻蓝蛋白（Phycocyanin， PC）是一种具有多种生物技术应用的色素蛋白复合物，在蓝藻光合作用的光能传递中起着关键作用。来自耐高温蓝藻振荡菌sp. N09DM的PC （O-PC）与中温蓝藻相比表现出显著的稳定性，使其在工业和医疗应用中具有很高的价值。为了了解其稳定性的基础，对O-PC的晶体结构进行了求解和分析。结构分析揭示了一个关键的分子相互作用，包括氢键、盐桥和疏水相互作用，以及提供热稳定性的氨基酸取代。此外，结构结果为理解O-PC在光能有效转移中的作用提供了对发色团-蛋白质相互作用的见解。

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Structure and stability of phycocyanin from thermotolerant Oscillatoria

Phycocyanin (PC), a pigment–protein complex with diverse biotechnological applications, plays a key role in light energy transfer for photosynthesis in cyanobacteria. PC (O-PC) from a thermotolerant cyanobacteria Oscillatoria sp. N09DM exhibits remarkable stability compared to its mesophilic counterparts, making it highly valuable for industrial and medical applications. To understand the basis of its stability, the crystal structure of O-PC is solved and analysed. Structural analysis reveals a key molecular interaction, including hydrogen bonds, salt bridges and hydrophobic interactions, along with amino acid substitutions that provide the thermal stability. Additionally, structural results provide insights into chromophore-protein interactions for understanding O-PC's role in the efficient transfer of light energy.

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来源期刊

FEBS Letters 生物-生化与分子生物学

CiteScore

7.00

自引率

2.90%

发文量

303

审稿时长

1.0 months

期刊介绍： FEBS Letters is one of the world''s leading journals in molecular biology and is renowned both for its quality of content and speed of production. Bringing together the most important developments in the molecular biosciences, FEBS Letters provides an international forum for Minireviews, Research Letters and Hypotheses that merit urgent publication.