miR-24-3p和miR-1277-5p对血液透析患者血管通路血栓形成的预测价值。

IF 1.2 4区 医学 Q4 HEMATOLOGY
Raziyeh Jalakani, Ali Khodadadi, Eisa Rezaei, Niloofar Motamed, Amir Hooshang Bavarsad, Seyed Amin Mohammadi, Zahra Mohammadi, Gholamreza Khamisipour
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引用次数: 0

摘要

慢性肾病(CKD)的并发症之一是静脉血栓栓塞(VTE)。目前,d -二聚体试验用于静脉血栓栓塞的诊断。该检查诊断价值和特异性较低。循环microrna存在于血浆、血清和其他体液中,最近被证明是许多疾病中有价值的生物标志物。因此,本研究旨在评估血清microrna作为血液透析患者静脉血栓栓塞诊断的无创生物标志物的诊断潜力。选取42例有血栓形成的血液透析患者、42例无血栓形成的血液透析患者和42例健康人群的血清样本。从血清中合成cDNA后,采用qRT-PCR检测miR-24-3P和miR-1277-5P的表达。采用SPSS 20和GraphPad Prism7软件对数据进行分析。经高血糖校正后,血栓性和非血栓性血液透析组miR-24-3P的表达水平均显著高于健康组(P = 0.003, P = 0.04)。受试者工作特征(ROC)分析显示,曲线下面积(AUC)分别为0.769和0.649。然而,在调整高血糖后,血栓形成组与非血栓形成组比较,无显著差异(P = 0.063), AUC为0.62。在调整年龄、性别和BMI后,血栓形成组和非血栓形成组之间存在显著差异(P = 0.002), AUC为0.71。与对照组相比,血栓形成组miR-1277-5P表达升高的比值比(OR)更大(P = 0.05, OR = 1.618)。非血栓形成组与对照组无显著性差异(P = 0.73, OR = 0.914)。我们的研究结果表明,miR-24-3P不是检测研究样本中血栓形成的可靠标志物,而miR-1277-5P与VAT呈正相关,可以被认为是诊断和治疗的标志物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Predictive value of miR-24-3p and miR-1277-5p in hemodialysis patients with vascular access thrombosis.

One of the complications of chronic kidney disease (CKD) is venous thromboembolism (VTE). Currently, the D-dimer test is used for the diagnosis of VTE. This test has low diagnostic value and specificity. Circulating microRNAs are present in plasma, serum, and other body fluids and have recently been shown to be valuable biomarkers in numerous illnesses. Therefore, this study aimed to evaluate the diagnostic potential of serum microRNAs as noninvasive biomarkers for VTE diagnosis in hemodialysis patients. Serum samples were collected from 42 hemodialysis patients with thrombosis, 42 hemodialysis patients without thrombosis, and 42 healthy individuals. After the synthesis of cDNA from serum, the expression of miR-24-3P and miR-1277-5P was measured by qRT-PCR. The data were analyzed using SPSS 20 and GraphPad Prism7 software. The expression level of miR-24-3P in the thrombotic and nonthrombotic hemodialysis groups was significantly greater than that in the healthy groups after adjustment for hyperglycemia (P = 0.003, P = 0.04). Receiver-operating characteristic (ROC) analysis revealed that the area under the curve (AUC) values were 0.769 and 0.649, respectively. However, in the thrombotic group compared with the nonthrombotic group after adjustment for hyperglycemia, no significant difference was detected (P = 0.063), and the AUC was 0.62. After adjustment for age, sex, and BMI, there was a significant difference between the thrombotic group and the nonthrombotic group (P = 0.002), and the AUC was 0.71. Compared with that in the control group, the odds ratio (OR) of increased miR-1277-5P expression was greater in the thrombotic group (P = 0.05, OR = 1.618). There was no significant difference between the nonthrombotic group and the control group (P = 0.73, OR = 0.914). Our results indicated that miR-24-3P is not a reliable marker for examining thrombosis in the studied samples, while miR-1277-5P has a positive association with VAT and could be considered a diagnostic and therapeutic marker.

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来源期刊
CiteScore
1.90
自引率
0.00%
发文量
111
审稿时长
4-8 weeks
期刊介绍: Blood Coagulation & Fibrinolysis is an international fully refereed journal that features review and original research articles on all clinical, laboratory and experimental aspects of haemostasis and thrombosis. The journal is devoted to publishing significant developments worldwide in the field of blood coagulation, fibrinolysis, thrombosis, platelets and the kininogen-kinin system, as well as dealing with those aspects of blood rheology relevant to haemostasis and the effects of drugs on haemostatic components
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