Sandrine Ninotta, David Toubeau, Tomy Sagnial, Zhiguo He, Sylvain Poinard, Gauthier Travers, Philippe Gain, Gilles Thuret, Marc Muraine
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In addition to ECD (in the three experiments), the viable ECD (Hoechst 33342/Calcein-AM staining) and central corneal thickness (CCT) were measured in exp. 2.</p><p><strong>Results: </strong>In experiment 1 (16 pairs), ECD was significantly higher in CorneaMax than in Tissue-C on D30 (2265 ± 379 vs. 2097 ± 344 cells/mm2, P = 0.0248) and postdeswelling (2106 ± 405 vs. 1910 ± 355 cells/mm2, P = 0.0290). In experiment 2 (10 pairs), ECD did not differ after 28 days of OC; however, CCT was lower on D4, higher on D28 in Tissue-C, and showed no difference after deswelling. Postdeswelling, viable ECD was significantly higher in CorneaJet versus Carry-C (1647 ± 324 vs. 1436 ± 235 cells/mm2, P = 0.0189). In experiment 3 (eight pairs), ECD showed no significant difference.</p><p><strong>Conclusions: </strong>Endothelial survival is reduced in Tissue-C/Carry-C unless storage is limited to 3 weeks and the medium is renewed. The major corneal edema in Tissue-C may contribute to the reduced ECD.</p>","PeriodicalId":10710,"journal":{"name":"Cornea","volume":" ","pages":""},"PeriodicalIF":1.9000,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Comparison of Two Commercial Medias for Corneal Organ Culture and Deswelling: CorneaMax/CorneaJet Versus Tissue-C/Carry-C.\",\"authors\":\"Sandrine Ninotta, David Toubeau, Tomy Sagnial, Zhiguo He, Sylvain Poinard, Gauthier Travers, Philippe Gain, Gilles Thuret, Marc Muraine\",\"doi\":\"10.1097/ICO.0000000000003876\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Purpose: </strong>To compare the endothelial cell density (ECD) of corneas stored in organ culture (OC) using CorneaMax/CorneaJet and Tissue-C/Carry-C media.</p><p><strong>Methods: </strong>Three complementary experiments included paired corneas stored in CorneaMax or Tissue-C and subsequently deswelled in CorneaJet or Carry-C. 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引用次数: 0
摘要
目的：比较CorneaMax/CorneaJet和Tissue-C/Carry-C培养基在器官培养（OC）中角膜内皮细胞密度（ECD）的差异。方法：三个互补实验，将配对的角膜保存在CorneaMax或Tissue-C中，随后在CorneaJet或Carry-C中进行肿胀。在实验1中，仅对CorneaMax进行存储介质更新，按照制造商的指南，存储/溶胀时间为30/4天(D)。实验2在第4天更新两种储存介质，储存/溶胀时间为28/2天。实验3对两种储存介质进行更新，储存/溶胀时间为22/4天。除ECD（在三个实验中）外，实验2测量了活ECD （Hoechst 33342/Calcein-AM染色）和角膜中央厚度（CCT）。结果：在实验1（16对）中，CorneaMax在D30（2265±379比2097±344细胞/mm2， P = 0.0248）和溶肿后（2106±405比1910±355细胞/mm2， P = 0.0290）的ECD显著高于Tissue-C。实验2（10对），28 d后ECD无差异；而组织c的CCT在D4时较低，D28时较高，消肿后无差异。去肿胀后，CorneaJet的活ECD显著高于Carry-C（1647±324比1436±235个细胞/mm2， P = 0.0189）。在实验3（8对）中，ECD无显著差异。结论：在Tissue-C/Carry-C中，内皮细胞存活率降低，除非储存限制为3周并更新培养基。c组织中主要的角膜水肿可能导致ECD降低。
Comparison of Two Commercial Medias for Corneal Organ Culture and Deswelling: CorneaMax/CorneaJet Versus Tissue-C/Carry-C.
Purpose: To compare the endothelial cell density (ECD) of corneas stored in organ culture (OC) using CorneaMax/CorneaJet and Tissue-C/Carry-C media.
Methods: Three complementary experiments included paired corneas stored in CorneaMax or Tissue-C and subsequently deswelled in CorneaJet or Carry-C. In experiment 1, storage media renewal was performed only for CorneaMax as per the manufacturer's guidelines, with storage/deswelling times of 30/4 days (D). In experiment 2, both storage media were renewed on D4, with storage/deswelling time of 28/2 days. In experiment 3, both storage media were renewed, with storage/deswelling times of 22/4 days. In addition to ECD (in the three experiments), the viable ECD (Hoechst 33342/Calcein-AM staining) and central corneal thickness (CCT) were measured in exp. 2.
Results: In experiment 1 (16 pairs), ECD was significantly higher in CorneaMax than in Tissue-C on D30 (2265 ± 379 vs. 2097 ± 344 cells/mm2, P = 0.0248) and postdeswelling (2106 ± 405 vs. 1910 ± 355 cells/mm2, P = 0.0290). In experiment 2 (10 pairs), ECD did not differ after 28 days of OC; however, CCT was lower on D4, higher on D28 in Tissue-C, and showed no difference after deswelling. Postdeswelling, viable ECD was significantly higher in CorneaJet versus Carry-C (1647 ± 324 vs. 1436 ± 235 cells/mm2, P = 0.0189). In experiment 3 (eight pairs), ECD showed no significant difference.
Conclusions: Endothelial survival is reduced in Tissue-C/Carry-C unless storage is limited to 3 weeks and the medium is renewed. The major corneal edema in Tissue-C may contribute to the reduced ECD.
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