Yulu Wu, Zhiyong Guo, Zhangao Li, Chenchen Cai, Jiyuan Liu, Xiufa Tang, Lin Que
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Effects of Integrin-Linked Kinase Silencing Combined With Trichostatin A on Cancer Stem Cells.
Objectives: Oral squamous cell carcinoma (OSCC) is characterized by high invasiveness and metastasis, with cancer stem cells (CSCs) playing a central role in tumor progression. This study investigates the effects of integrin-linked kinase (ILK) silencing and trichostatin A (TSA) treatment on CSCs, assessing their potential to diminish CSC properties and inhibit OSCC progression.
Methods and materials: CSCs were enriched and isolated from primary OSCC samples and Tca8113 cell line and MOC1 cell line using side population (SP) analysis, with their characteristics and the therapeutic impact of treatments assessed through assays such as MTT, wound healing, cell invasion, cell cycle, and apoptosis.
Results: Higher SP cell content correlated significantly with poor pathological classification, metastasis, and recurrence. Treated CSCs showed reduced proliferation, migration, and invasion, along with increased apoptosis. In vivo experiments demonstrated that the combined treatment substantially reduced tumor growth.
Conclusion: The study confirms the efficacy of targeting CSCs with ILK silencing and TSA treatment in OSCC, suggesting a promising strategy for CSC-directed therapies that merit further investigation.
期刊介绍：
Oral Diseases is a multidisciplinary and international journal with a focus on head and neck disorders, edited by leaders in the field, Professor Giovanni Lodi (Editor-in-Chief, Milan, Italy), Professor Stefano Petti (Deputy Editor, Rome, Italy) and Associate Professor Gulshan Sunavala-Dossabhoy (Deputy Editor, Shreveport, LA, USA). The journal is pre-eminent in oral medicine. Oral Diseases specifically strives to link often-isolated areas of dentistry and medicine through broad-based scholarship that includes well-designed and controlled clinical research, analytical epidemiology, and the translation of basic science in pre-clinical studies. The journal typically publishes articles relevant to many related medical specialties including especially dermatology, gastroenterology, hematology, immunology, infectious diseases, neuropsychiatry, oncology and otolaryngology. The essential requirement is that all submitted research is hypothesis-driven, with significant positive and negative results both welcomed. Equal publication emphasis is placed on etiology, pathogenesis, diagnosis, prevention and treatment.
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