葡萄球菌肠毒素A处理对T细胞相关A20表达下调的影响

IF 0.9 4区 医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL
X Chen, Y Yan, C Lin, J Yang, H Qiu
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引用次数: 0

摘要

A20是NF-κB信号的负调控因子,是一种有效的抗炎分子。其缺乏与多种炎症性疾病和肿瘤有关,A20限制TCR-NF-κB信号通路的能力及其在t细胞白血病发病机制中的作用尚不完全清楚。在这里,我们研究了A20在暴露于葡萄球菌肠毒素A (SEA)的T细胞中的作用,并评估了长期低剂量SEA对Jurkat细胞、人外周血单个核细胞和CD3+ T细胞的致死性炎症的体外研究结果。SEA治疗导致Jurkat细胞慢性炎症,上调MALT1、IKKβ和p65的表达,下调A20的表达,无论mRNA或蛋白水平如何,均呈剂量和时间依赖性。因此,sea介导的慢性炎症可通过下调A20表达激活TCR-NF-κB信号,从而增加t细胞不朽性,可能促进t细胞白血病的发病。调节A20可能是治疗t细胞白血病的一种新策略。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Downregulation of A20 Expression Related to T Cells by Staphylococcal Enterotoxin A Treatment.

A20, a negative regulator of NF-κB signaling, is a potent anti-inflammatory molecule. Its deficiency is associated with a wide variety of inflammatory diseases and tumors and the ability of A20 to restrict TCR-NF-κB signaling pathway and the role of this molecule in the pathogenesis of T-cell leukemia are not completely understood. Here we studied the role of A20 in T cells exposed to staphylococcal enterotoxin A (SEA) and evaluated the results of our in vitro findings of lethal inflammation by long-term administration of SEA at low doses to Jurkat cells, human peripheral blood mononuclear cells, and CD3+ T cells. SEA treatment resulted in chronic inflammation, upregulated the expression of MALT1, IKKβ, and p65, and downregulated the expression of A20, both in dose- and time-dependent manners, in Jurkat cells, regardless of the mRNA or protein level. Thus, SEA-mediated chronic inflammation can activate TCR-NF-κB signals via the downregulation of A20 expression, which increases T-cell immortality and may promote the pathogenesis of T-cell leukemia. Modulation of A20 could be a novel strategy for the treatment of T-cell leukemia.

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来源期刊
Bulletin of Experimental Biology and Medicine
Bulletin of Experimental Biology and Medicine 医学-医学:研究与实验
CiteScore
1.50
自引率
14.30%
发文量
265
审稿时长
2 months
期刊介绍: Bulletin of Experimental Biology and Medicine presents original peer reviewed research papers and brief reports on priority new research results in physiology, biochemistry, biophysics, pharmacology, immunology, microbiology, genetics, oncology, etc. Novel trends in science are covered in new sections of the journal - Biogerontology and Human Ecology - that first appeared in 2005. World scientific interest in stem cells prompted inclusion into Bulletin of Experimental Biology and Medicine a quarterly scientific journal Cell Technologies in Biology and Medicine (a new Russian Academy of Medical Sciences publication since 2005). It publishes only original papers from the leading research institutions on molecular biology of stem and progenitor cells, stem cell as the basis of gene therapy, molecular language of cell-to-cell communication, cytokines, chemokines, growth and other factors, pilot projects on clinical use of stem and progenitor cells. The Russian Volume Year is published in English from April.
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