珍妮围生期肠道菌群的动态变化。

IF 2.7 2区 农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Animals Pub Date : 2025-05-06 DOI:10.3390/ani15091337
Xinyue Wang, Yang Shao, Xiaoling Zhou, Zheng Li, Jingze Liu, Mingyao Tang, Yixin Yang, Liang Deng
{"title":"珍妮围生期肠道菌群的动态变化。","authors":"Xinyue Wang, Yang Shao, Xiaoling Zhou, Zheng Li, Jingze Liu, Mingyao Tang, Yixin Yang, Liang Deng","doi":"10.3390/ani15091337","DOIUrl":null,"url":null,"abstract":"<p><p>The peripartum period is critical for breeding female donkeys (i.e., jennies) and ensuring the delivery of healthy neonatal foals. The gut microbiota deeply influences the host metabolism. This study aimed to investigate the dynamic changes in the gut microbiome during the peripartum period in jennies. Fresh fecal samples of eight adult jennies were collected at the following seven sampling time points: 21, 7, and 3 days prepartum (G21, G7, and G3) and 1, 3, 7, and 14 days postpartum (L1, L3, L7, and L14). Sequencing of the V4 hypervariable regions of the 16S rRNA genes was carried out using fecal samples to identify the differences in the microbiome across the peripartum period. Bacteroidota and Firmicutes were the most abundant bacterial phyla in the feces. <i>Treponema</i> and <i>Lachnospiraceae XPB1014 group</i> significantly increased in the L3 group compared to the G7 group (<i>q</i> < 0.05), and a decline trend was observed in L1 group around parturition. The genus <i>Clostridium sensu stricto 1</i>, family Clostridiaceae, and order Clostridiales were considered to be biomarkers of the L3 group. Among the 25 functional pathways detected by Kyoto Encyclopedia of Genes and Genomes pathway analysis, beta lactam resistance, insulin resistance, and peptidases were the top three important pathways observed in the gut microbiota during the peripartum period in jennies. The gut microbial structure changed significantly at different time points during the peripartum period in jennies. These results contribute to a better understanding of the gut microbiota to ensure health care during important phases from late pregnancy to early lactation in jennies.</p>","PeriodicalId":7955,"journal":{"name":"Animals","volume":"15 9","pages":""},"PeriodicalIF":2.7000,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12071091/pdf/","citationCount":"0","resultStr":"{\"title\":\"Dynamic Changes in the Gut Microbiota During Peripartum in Jennies.\",\"authors\":\"Xinyue Wang, Yang Shao, Xiaoling Zhou, Zheng Li, Jingze Liu, Mingyao Tang, Yixin Yang, Liang Deng\",\"doi\":\"10.3390/ani15091337\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The peripartum period is critical for breeding female donkeys (i.e., jennies) and ensuring the delivery of healthy neonatal foals. The gut microbiota deeply influences the host metabolism. This study aimed to investigate the dynamic changes in the gut microbiome during the peripartum period in jennies. Fresh fecal samples of eight adult jennies were collected at the following seven sampling time points: 21, 7, and 3 days prepartum (G21, G7, and G3) and 1, 3, 7, and 14 days postpartum (L1, L3, L7, and L14). Sequencing of the V4 hypervariable regions of the 16S rRNA genes was carried out using fecal samples to identify the differences in the microbiome across the peripartum period. Bacteroidota and Firmicutes were the most abundant bacterial phyla in the feces. <i>Treponema</i> and <i>Lachnospiraceae XPB1014 group</i> significantly increased in the L3 group compared to the G7 group (<i>q</i> < 0.05), and a decline trend was observed in L1 group around parturition. The genus <i>Clostridium sensu stricto 1</i>, family Clostridiaceae, and order Clostridiales were considered to be biomarkers of the L3 group. Among the 25 functional pathways detected by Kyoto Encyclopedia of Genes and Genomes pathway analysis, beta lactam resistance, insulin resistance, and peptidases were the top three important pathways observed in the gut microbiota during the peripartum period in jennies. The gut microbial structure changed significantly at different time points during the peripartum period in jennies. These results contribute to a better understanding of the gut microbiota to ensure health care during important phases from late pregnancy to early lactation in jennies.</p>\",\"PeriodicalId\":7955,\"journal\":{\"name\":\"Animals\",\"volume\":\"15 9\",\"pages\":\"\"},\"PeriodicalIF\":2.7000,\"publicationDate\":\"2025-05-06\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12071091/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Animals\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.3390/ani15091337\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRICULTURE, DAIRY & ANIMAL SCIENCE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Animals","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.3390/ani15091337","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}
引用次数: 0

摘要

围产期对于母驴(即珍妮)的繁殖和确保产下健康的新生马驹至关重要。肠道菌群深刻影响宿主的代谢。本研究旨在探讨围生期孕妇肠道菌群的动态变化。在以下7个采样时间点采集8只成年珍妮的新鲜粪便样本:产前21、7和3天(G21、G7和G3)和产后1、3、7和14天(L1、L3、L7和L14)。利用粪便样本对16S rRNA基因的V4高变区进行测序,以确定围生期微生物组的差异。粪便中数量最多的细菌门是拟杆菌门和厚壁菌门。Treponema和Lachnospiraceae XPB1014组在L3组较G7组显著升高(q < 0.05), L1组在分娩前后有下降趋势。严格感梭状芽孢杆菌属1、梭状芽孢杆菌科和梭状芽孢杆菌目被认为是L3组的生物标志物。在《京都基因与基因组百科全书》通路分析中检测到的25条功能通路中,β -内酰胺抵抗、胰岛素抵抗和肽酶是围生期孕妇肠道微生物群中最重要的3条通路。围生期孕妇肠道微生物结构在不同时间点发生显著变化。这些结果有助于更好地了解肠道微生物群,以确保从怀孕晚期到哺乳期早期的重要阶段的卫生保健。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Dynamic Changes in the Gut Microbiota During Peripartum in Jennies.

The peripartum period is critical for breeding female donkeys (i.e., jennies) and ensuring the delivery of healthy neonatal foals. The gut microbiota deeply influences the host metabolism. This study aimed to investigate the dynamic changes in the gut microbiome during the peripartum period in jennies. Fresh fecal samples of eight adult jennies were collected at the following seven sampling time points: 21, 7, and 3 days prepartum (G21, G7, and G3) and 1, 3, 7, and 14 days postpartum (L1, L3, L7, and L14). Sequencing of the V4 hypervariable regions of the 16S rRNA genes was carried out using fecal samples to identify the differences in the microbiome across the peripartum period. Bacteroidota and Firmicutes were the most abundant bacterial phyla in the feces. Treponema and Lachnospiraceae XPB1014 group significantly increased in the L3 group compared to the G7 group (q < 0.05), and a decline trend was observed in L1 group around parturition. The genus Clostridium sensu stricto 1, family Clostridiaceae, and order Clostridiales were considered to be biomarkers of the L3 group. Among the 25 functional pathways detected by Kyoto Encyclopedia of Genes and Genomes pathway analysis, beta lactam resistance, insulin resistance, and peptidases were the top three important pathways observed in the gut microbiota during the peripartum period in jennies. The gut microbial structure changed significantly at different time points during the peripartum period in jennies. These results contribute to a better understanding of the gut microbiota to ensure health care during important phases from late pregnancy to early lactation in jennies.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Animals
Animals Agricultural and Biological Sciences-Animal Science and Zoology
CiteScore
4.90
自引率
16.70%
发文量
3015
审稿时长
20.52 days
期刊介绍: Animals (ISSN 2076-2615) is an international and interdisciplinary scholarly open access journal. It publishes original research articles, reviews, communications, and short notes that are relevant to any field of study that involves animals, including zoology, ethnozoology, animal science, animal ethics and animal welfare. However, preference will be given to those articles that provide an understanding of animals within a larger context (i.e., the animals'' interactions with the outside world, including humans). There is no restriction on the length of the papers. Our aim is to encourage scientists to publish their experimental and theoretical research in as much detail as possible. Full experimental details and/or method of study, must be provided for research articles. Articles submitted that involve subjecting animals to unnecessary pain or suffering will not be accepted, and all articles must be submitted with the necessary ethical approval (please refer to the Ethical Guidelines for more information).
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信