{"title":"3株内生芽孢杆菌对玉米黄曲霉抑制作用的研究。","authors":"Siyu Ma, Min Li, Siqi Zhang, Yin Yang, Fengsha Zhu, Xingyu Li, Shahzad Munir, Pengfei He, Pengbo He, Yixin Wu, Yueqiu He, Ping Tang","doi":"10.3390/metabo15040268","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Maize is easily contaminated by <i>Aspergillus flavus</i>, and the aflatoxin produced by <i>A. flavus</i> has been classified as a Group 1 carcinogen, for which there are currently no effective control measures. Biological control is regarded as an environmentally friendly and safe approach. Strains ZH179, ZH409, and ZH99 are three bacteria isolated from our laboratory that exhibit antagonistic effects against <i>A. flavus</i>. We conducted experiments to investigate their biocontrol efficacy.</p><p><strong>Results: </strong>The experimental results demonstrated that these three strains effectively inhibited <i>A. flavus</i> on plates and stored maize seeds. Identification revealed that ZH179 is <i>Bacillus velezensis</i>, while ZH409 and ZH99 are <i>B. amyloliquefaciens</i>. We also identified lipopeptide synthetase-related genes, including <i>srfAA</i>, <i>srfAD</i>, <i>fenA</i>, <i>fenB</i>, <i>ituA</i>, <i>ituB</i>, <i>ituD</i>, <i>bmyA</i>, <i>bmyB</i>, and <i>bmyC</i>, in these three strains. Furthermore, LC-MS analysis confirmed that these strains could produce lipopeptide compounds such as surfactin, fengycin, iturin, and bacillomycin. Using the Oxford cup method, we found that the lipopeptide compounds produced by these strains can inhibit the growth of <i>A. flavus</i>.</p><p><strong>Conclusion: </strong>These findings suggest that strains ZH179, ZH409, and ZH99 have good control effects on <i>A. flavus</i> during the storage of maize, primarily due to the lipopeptide compounds. This study provides a theoretical basis for using these three strains in the biological control of <i>A. flavus</i>.</p>","PeriodicalId":18496,"journal":{"name":"Metabolites","volume":"15 4","pages":""},"PeriodicalIF":3.4000,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12029785/pdf/","citationCount":"0","resultStr":"{\"title\":\"Study on the Inhibitory Effects of Three Endophytic <i>Bacillus</i> Strains on <i>Aspergillus flavus</i> in Maize.\",\"authors\":\"Siyu Ma, Min Li, Siqi Zhang, Yin Yang, Fengsha Zhu, Xingyu Li, Shahzad Munir, Pengfei He, Pengbo He, Yixin Wu, Yueqiu He, Ping Tang\",\"doi\":\"10.3390/metabo15040268\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Maize is easily contaminated by <i>Aspergillus flavus</i>, and the aflatoxin produced by <i>A. flavus</i> has been classified as a Group 1 carcinogen, for which there are currently no effective control measures. Biological control is regarded as an environmentally friendly and safe approach. Strains ZH179, ZH409, and ZH99 are three bacteria isolated from our laboratory that exhibit antagonistic effects against <i>A. flavus</i>. We conducted experiments to investigate their biocontrol efficacy.</p><p><strong>Results: </strong>The experimental results demonstrated that these three strains effectively inhibited <i>A. flavus</i> on plates and stored maize seeds. Identification revealed that ZH179 is <i>Bacillus velezensis</i>, while ZH409 and ZH99 are <i>B. amyloliquefaciens</i>. We also identified lipopeptide synthetase-related genes, including <i>srfAA</i>, <i>srfAD</i>, <i>fenA</i>, <i>fenB</i>, <i>ituA</i>, <i>ituB</i>, <i>ituD</i>, <i>bmyA</i>, <i>bmyB</i>, and <i>bmyC</i>, in these three strains. Furthermore, LC-MS analysis confirmed that these strains could produce lipopeptide compounds such as surfactin, fengycin, iturin, and bacillomycin. Using the Oxford cup method, we found that the lipopeptide compounds produced by these strains can inhibit the growth of <i>A. flavus</i>.</p><p><strong>Conclusion: </strong>These findings suggest that strains ZH179, ZH409, and ZH99 have good control effects on <i>A. flavus</i> during the storage of maize, primarily due to the lipopeptide compounds. This study provides a theoretical basis for using these three strains in the biological control of <i>A. flavus</i>.</p>\",\"PeriodicalId\":18496,\"journal\":{\"name\":\"Metabolites\",\"volume\":\"15 4\",\"pages\":\"\"},\"PeriodicalIF\":3.4000,\"publicationDate\":\"2025-04-11\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12029785/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Metabolites\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.3390/metabo15040268\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"BIOCHEMISTRY & MOLECULAR BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Metabolites","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.3390/metabo15040268","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
Study on the Inhibitory Effects of Three Endophytic Bacillus Strains on Aspergillus flavus in Maize.
Background: Maize is easily contaminated by Aspergillus flavus, and the aflatoxin produced by A. flavus has been classified as a Group 1 carcinogen, for which there are currently no effective control measures. Biological control is regarded as an environmentally friendly and safe approach. Strains ZH179, ZH409, and ZH99 are three bacteria isolated from our laboratory that exhibit antagonistic effects against A. flavus. We conducted experiments to investigate their biocontrol efficacy.
Results: The experimental results demonstrated that these three strains effectively inhibited A. flavus on plates and stored maize seeds. Identification revealed that ZH179 is Bacillus velezensis, while ZH409 and ZH99 are B. amyloliquefaciens. We also identified lipopeptide synthetase-related genes, including srfAA, srfAD, fenA, fenB, ituA, ituB, ituD, bmyA, bmyB, and bmyC, in these three strains. Furthermore, LC-MS analysis confirmed that these strains could produce lipopeptide compounds such as surfactin, fengycin, iturin, and bacillomycin. Using the Oxford cup method, we found that the lipopeptide compounds produced by these strains can inhibit the growth of A. flavus.
Conclusion: These findings suggest that strains ZH179, ZH409, and ZH99 have good control effects on A. flavus during the storage of maize, primarily due to the lipopeptide compounds. This study provides a theoretical basis for using these three strains in the biological control of A. flavus.
MetabolitesBiochemistry, Genetics and Molecular Biology-Molecular Biology
CiteScore
5.70
自引率
7.30%
发文量
1070
审稿时长
17.17 days
期刊介绍：
Metabolites (ISSN 2218-1989) is an international, peer-reviewed open access journal of metabolism and metabolomics. Metabolites publishes original research articles and review articles in all molecular aspects of metabolism relevant to the fields of metabolomics, metabolic biochemistry, computational and systems biology, biotechnology and medicine, with a particular focus on the biological roles of metabolites and small molecule biomarkers. Metabolites encourages scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on article length. Sufficient experimental details must be provided to enable the results to be accurately reproduced. Electronic material representing additional figures, materials and methods explanation, or supporting results and evidence can be submitted with the main manuscript as supplementary material.
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