来自人脐带血间充质干细胞的外泌体通过抑制FGF2的表达来保护视网膜色素上皮细胞免受蓝光诱导的损伤。

IF 2 4区生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Cytotechnology Pub Date : 2025-06-01 Epub Date: 2025-04-09 DOI:10.1007/s10616-025-00752-4

Guang-Ming Liu, Yan Liu

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引用次数: 0

摘要

年龄相关性黄斑变性（AMD）是一种使视网膜衰弱的疾病，可能导致进行性视力丧失。AMD发病的一个重要因素是过量的蓝光照射。在这项研究中，我们研究了来自人脐带血间充质干细胞（hUCMSC-EXs）的外泌体在解决bl诱导的ARPE-19人视网膜色素上皮（RPE）细胞损伤中的治疗潜力，并探讨了潜在的机制。我们的研究结果显示，BL暴露诱导ARPE-19细胞形态学改变，伴随着细胞活力的时间依赖性下降，细胞凋亡增加，氧化应激增强和炎症反应；然而，hUCMSC-EXs剂量依赖性地减轻了bl诱导的ARPE-19细胞损伤。有趣的是，在暴露于bl的ARPE-19细胞中，hUCMSC-EXs被发现抑制成纤维细胞生长因子2 （FGF2）的上调。此外，FGF2过表达部分抵消了hUCMSC-EXs对FGF2表达的抑制作用，削弱了hUCMSC-EXs对bl诱导的ARPE-19细胞损伤的保护作用。总之，我们的研究结果表明，hUCMSC-EXs通过抑制FGF2的表达来保护ARPE-19细胞免受bl诱导的伤害。

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Exosomes derived from human umbilical cord blood mesenchymal stem cells protect against blue light-induced damage to retinal pigment epithelial cells by inhibiting FGF2 expression.

Age-related macular degeneration (AMD) is a debilitating retinal disorder that may lead to progressive vision loss. One contributing factor to AMD pathogenesis is excessive blue light (BL) exposure. In this study, we investigated the therapeutic potential of exosomes derived from human umbilical cord blood mesenchymal stem cells (hUCMSC-EXs) in addressing BL-induced damage to ARPE-19 human retinal pigment epithelial (RPE) cells and explored the underlying mechanisms. Our findings revealed that BL exposure induced morphological alterations in ARPE-19 cells, accompanied by a time-dependent decline in cell viability, increased apoptosis, heightened oxidative stress, and inflammatory responses; however, hUCMSC-EXs dose-dependently mitigated BL-induced ARPE-19 cell damage. Interestingly, hUCMSC-EXs were found to suppress the upregulation of fibroblast growth factor 2 (FGF2) in BL-exposed ARPE-19 cells. Furthermore, FGF2 overexpression partially counteracted the inhibitory effects of hUCMSC-EXs on FGF2 expression and compromised the protective benefits of hUCMSC-EXs against BL-induced ARPE-19 cell damage. In conclusion, our results suggest that hUCMSC-EXs shield ARPE-19 cells from BL-induced harm by inhibiting FGF2 expression.

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来源期刊

Cytotechnology 生物-生物工程与应用微生物

CiteScore

4.10

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： The scope of the Journal includes: 1. The derivation, genetic modification and characterization of cell lines, genetic and phenotypic regulation, control of cellular metabolism, cell physiology and biochemistry related to cell function, performance and expression of cell products. 2. Cell culture techniques, substrates, environmental requirements and optimization, cloning, hybridization and molecular biology, including genomic and proteomic tools. 3. Cell culture systems, processes, reactors, scale-up, and industrial production. Descriptions of the design or construction of equipment, media or quality control procedures, that are ancillary to cellular research. 4. The application of animal/human cells in research in the field of stem cell research including maintenance of stemness, differentiation, genetics, and senescence, cancer research, research in immunology, as well as applications in tissue engineering and gene therapy. 5. The use of cell cultures as a substrate for bioassays, biomedical applications and in particular as a replacement for animal models.