{"title":"两种免疫去势疫苗的制备及其对公山羊的免疫效果。","authors":"Fuqiang Pan, Yumeng Guo, Panpan Cheng, Wei Qian, Mengdi Han, Qing Yi, Huihui Xie, Meng Cao, Yanqiuhong Li, Yuke Jia, Jiankun Cui, Xinbao Gong, Ziye Zhu, Fugui Fang, Yinghui Ling, Yunsheng Li, Jian Li, Ya Liu","doi":"10.5713/ab.24.0811","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>After sexual maturity, male goats will exhibit fighting and mounting behaviors due to the increase of testosterone level, resulting in slower growth rate and mutton odor. However, traditional castration methods will cause significant stress. In contrast, immunocastration is a welfare-friendly and gentler approach to replace mechanical castration. Therefore, it is important to develop immunocastration vaccines with good immune effects.</p><p><strong>Methods: </strong>GnRH octamer vaccine (G8), LHR and GnRH octamer tandem vaccine (LG) were designed and prepared for this study. Forty 3-month-old male goats were randomly divided into four groups: surgical castration (SC group), immunization with the G8 vaccine (G8 group), immunization with the LG vaccine (LG group), or left intact (non- castration, NC group). After the first immunization, serum antibodies and testosterone levels, as well as body weight, body size, and scrotal size, were measured at different times. Testicular size and slaughter rate were measured at slaughter.</p><p><strong>Results: </strong>Both vaccines successfully elicited the corresponding antibodies in male goats; the testosterone levels in the G8 and LG groups were significantly lower than those in the NC group (p< 0.01) 4 weeks after the first immunization and this trend continued until the end of the experiment; the testicular organ index and size of G8 group were significantly smaller than those of NC group at slaughter (p < 0.05). Compared with the NC group, the seminiferous tubule diameter in the G8 and LG groups was significantly reduced (p < 0.01), with a notable decrease in Leydig Cells and various stages of spermatogenic cells, and the weight gain of goats in the SC group was significantly lower than that of other groups 2 weeks after the first immunization (p < 0.05).</p><p><strong>Conclusion: </strong>The two immunocastration vaccines developed can effectively inhibit the development of testes and spermatogenesis in male goats and reduce testosterone levels.</p>","PeriodicalId":7825,"journal":{"name":"Animal Bioscience","volume":" ","pages":""},"PeriodicalIF":2.4000,"publicationDate":"2025-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Preparation of two kinds of immunocastration vaccines and their immune effects on male goats.\",\"authors\":\"Fuqiang Pan, Yumeng Guo, Panpan Cheng, Wei Qian, Mengdi Han, Qing Yi, Huihui Xie, Meng Cao, Yanqiuhong Li, Yuke Jia, Jiankun Cui, Xinbao Gong, Ziye Zhu, Fugui Fang, Yinghui Ling, Yunsheng Li, Jian Li, Ya Liu\",\"doi\":\"10.5713/ab.24.0811\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Objective: </strong>After sexual maturity, male goats will exhibit fighting and mounting behaviors due to the increase of testosterone level, resulting in slower growth rate and mutton odor. However, traditional castration methods will cause significant stress. In contrast, immunocastration is a welfare-friendly and gentler approach to replace mechanical castration. Therefore, it is important to develop immunocastration vaccines with good immune effects.</p><p><strong>Methods: </strong>GnRH octamer vaccine (G8), LHR and GnRH octamer tandem vaccine (LG) were designed and prepared for this study. Forty 3-month-old male goats were randomly divided into four groups: surgical castration (SC group), immunization with the G8 vaccine (G8 group), immunization with the LG vaccine (LG group), or left intact (non- castration, NC group). After the first immunization, serum antibodies and testosterone levels, as well as body weight, body size, and scrotal size, were measured at different times. Testicular size and slaughter rate were measured at slaughter.</p><p><strong>Results: </strong>Both vaccines successfully elicited the corresponding antibodies in male goats; the testosterone levels in the G8 and LG groups were significantly lower than those in the NC group (p< 0.01) 4 weeks after the first immunization and this trend continued until the end of the experiment; the testicular organ index and size of G8 group were significantly smaller than those of NC group at slaughter (p < 0.05). Compared with the NC group, the seminiferous tubule diameter in the G8 and LG groups was significantly reduced (p < 0.01), with a notable decrease in Leydig Cells and various stages of spermatogenic cells, and the weight gain of goats in the SC group was significantly lower than that of other groups 2 weeks after the first immunization (p < 0.05).</p><p><strong>Conclusion: </strong>The two immunocastration vaccines developed can effectively inhibit the development of testes and spermatogenesis in male goats and reduce testosterone levels.</p>\",\"PeriodicalId\":7825,\"journal\":{\"name\":\"Animal Bioscience\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":2.4000,\"publicationDate\":\"2025-04-11\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Animal Bioscience\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.5713/ab.24.0811\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRICULTURE, DAIRY & ANIMAL SCIENCE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Animal Bioscience","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.5713/ab.24.0811","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}
Preparation of two kinds of immunocastration vaccines and their immune effects on male goats.
Objective: After sexual maturity, male goats will exhibit fighting and mounting behaviors due to the increase of testosterone level, resulting in slower growth rate and mutton odor. However, traditional castration methods will cause significant stress. In contrast, immunocastration is a welfare-friendly and gentler approach to replace mechanical castration. Therefore, it is important to develop immunocastration vaccines with good immune effects.
Methods: GnRH octamer vaccine (G8), LHR and GnRH octamer tandem vaccine (LG) were designed and prepared for this study. Forty 3-month-old male goats were randomly divided into four groups: surgical castration (SC group), immunization with the G8 vaccine (G8 group), immunization with the LG vaccine (LG group), or left intact (non- castration, NC group). After the first immunization, serum antibodies and testosterone levels, as well as body weight, body size, and scrotal size, were measured at different times. Testicular size and slaughter rate were measured at slaughter.
Results: Both vaccines successfully elicited the corresponding antibodies in male goats; the testosterone levels in the G8 and LG groups were significantly lower than those in the NC group (p< 0.01) 4 weeks after the first immunization and this trend continued until the end of the experiment; the testicular organ index and size of G8 group were significantly smaller than those of NC group at slaughter (p < 0.05). Compared with the NC group, the seminiferous tubule diameter in the G8 and LG groups was significantly reduced (p < 0.01), with a notable decrease in Leydig Cells and various stages of spermatogenic cells, and the weight gain of goats in the SC group was significantly lower than that of other groups 2 weeks after the first immunization (p < 0.05).
Conclusion: The two immunocastration vaccines developed can effectively inhibit the development of testes and spermatogenesis in male goats and reduce testosterone levels.
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