用于高空间带宽产品分析成像的圆顶apic照明设计。

IF 2.9 2区 医学 Q2 BIOCHEMICAL RESEARCH METHODS
Biomedical optics express Pub Date : 2025-03-27 eCollection Date: 2025-04-01 DOI:10.1364/BOE.555541
Siyu Steven Lin, Haowen Zhou, Ruizhi Cao, Shi Zhao, Oumeng Zhang, Changhuei Yang
{"title":"用于高空间带宽产品分析成像的圆顶apic照明设计。","authors":"Siyu Steven Lin, Haowen Zhou, Ruizhi Cao, Shi Zhao, Oumeng Zhang, Changhuei Yang","doi":"10.1364/BOE.555541","DOIUrl":null,"url":null,"abstract":"<p><p>Breaking the tradeoff between resolution and field-of-view, while obtaining distortion-free images, can be achieved through computational imaging techniques. A recent approach, Angular Ptychographic Imaging with Close-form method (APIC), has showcased its capability to analytically recover both intricate aberrations and high space-bandwidth product complex optical fields with NA-matching and darkfield illuminations. However, its flat illumination setup limits its ability to efficiently reconstruct a large field-of-view simultaneously with high resolution, owing to the curvature in the wavefront from NA-matching illuminations and the finite beam angle of the Lambertian LED light source. Here, we introduce an illumination framework tailored for APIC consisting of a distant annular LED ring and an LED dome that enables the reconstruction of a larger area with an extended synthetic numerical aperture, consequently enhancing resolution. For a single set of measurements, our new prototype, termed Dome-APIC can reach 620nm resolution with a 10×/0.25 NA objective lens over a field-of-view of 450 <i>µ</i>m x 450 <i>µ</i>m.</p>","PeriodicalId":8969,"journal":{"name":"Biomedical optics express","volume":"16 4","pages":"1666-1677"},"PeriodicalIF":2.9000,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12047733/pdf/","citationCount":"0","resultStr":"{\"title\":\"Dome-APIC illumination design for high space-bandwidth product analytic imaging.\",\"authors\":\"Siyu Steven Lin, Haowen Zhou, Ruizhi Cao, Shi Zhao, Oumeng Zhang, Changhuei Yang\",\"doi\":\"10.1364/BOE.555541\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Breaking the tradeoff between resolution and field-of-view, while obtaining distortion-free images, can be achieved through computational imaging techniques. A recent approach, Angular Ptychographic Imaging with Close-form method (APIC), has showcased its capability to analytically recover both intricate aberrations and high space-bandwidth product complex optical fields with NA-matching and darkfield illuminations. However, its flat illumination setup limits its ability to efficiently reconstruct a large field-of-view simultaneously with high resolution, owing to the curvature in the wavefront from NA-matching illuminations and the finite beam angle of the Lambertian LED light source. Here, we introduce an illumination framework tailored for APIC consisting of a distant annular LED ring and an LED dome that enables the reconstruction of a larger area with an extended synthetic numerical aperture, consequently enhancing resolution. For a single set of measurements, our new prototype, termed Dome-APIC can reach 620nm resolution with a 10×/0.25 NA objective lens over a field-of-view of 450 <i>µ</i>m x 450 <i>µ</i>m.</p>\",\"PeriodicalId\":8969,\"journal\":{\"name\":\"Biomedical optics express\",\"volume\":\"16 4\",\"pages\":\"1666-1677\"},\"PeriodicalIF\":2.9000,\"publicationDate\":\"2025-03-27\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12047733/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biomedical optics express\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1364/BOE.555541\",\"RegionNum\":2,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2025/4/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q2\",\"JCRName\":\"BIOCHEMICAL RESEARCH METHODS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomedical optics express","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1364/BOE.555541","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/4/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
引用次数: 0

摘要

打破分辨率和视场之间的权衡,同时获得无失真的图像,可以通过计算成像技术实现。最近的一种方法,角平面成像与近形式方法(APIC),已经展示了其分析恢复复杂像差和高空间带宽产品复杂光场与na匹配和暗场照明的能力。然而,由于na匹配照明的波前曲率和兰伯LED光源的有限光束角度,其平面照明设置限制了其同时以高分辨率有效重建大视场的能力。在这里,我们介绍了一个为APIC量身定制的照明框架,由一个遥远的环形LED环和一个LED圆顶组成,可以用扩展的合成数值孔径重建更大的区域,从而提高分辨率。对于一组测量,我们的新原型,称为Dome-APIC,可以在450 μ m x 450 μ m的视场上使用10×/0.25 NA的物镜达到620nm的分辨率。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Dome-APIC illumination design for high space-bandwidth product analytic imaging.

Breaking the tradeoff between resolution and field-of-view, while obtaining distortion-free images, can be achieved through computational imaging techniques. A recent approach, Angular Ptychographic Imaging with Close-form method (APIC), has showcased its capability to analytically recover both intricate aberrations and high space-bandwidth product complex optical fields with NA-matching and darkfield illuminations. However, its flat illumination setup limits its ability to efficiently reconstruct a large field-of-view simultaneously with high resolution, owing to the curvature in the wavefront from NA-matching illuminations and the finite beam angle of the Lambertian LED light source. Here, we introduce an illumination framework tailored for APIC consisting of a distant annular LED ring and an LED dome that enables the reconstruction of a larger area with an extended synthetic numerical aperture, consequently enhancing resolution. For a single set of measurements, our new prototype, termed Dome-APIC can reach 620nm resolution with a 10×/0.25 NA objective lens over a field-of-view of 450 µm x 450 µm.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Biomedical optics express
Biomedical optics express BIOCHEMICAL RESEARCH METHODS-OPTICS
CiteScore
6.80
自引率
11.80%
发文量
633
审稿时长
1 months
期刊介绍: The journal''s scope encompasses fundamental research, technology development, biomedical studies and clinical applications. BOEx focuses on the leading edge topics in the field, including: Tissue optics and spectroscopy Novel microscopies Optical coherence tomography Diffuse and fluorescence tomography Photoacoustic and multimodal imaging Molecular imaging and therapies Nanophotonic biosensing Optical biophysics/photobiology Microfluidic optical devices Vision research.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信