Siyu Steven Lin, Haowen Zhou, Ruizhi Cao, Shi Zhao, Oumeng Zhang, Changhuei Yang
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引用次数: 0
摘要
打破分辨率和视场之间的权衡,同时获得无失真的图像,可以通过计算成像技术实现。最近的一种方法,角平面成像与近形式方法(APIC),已经展示了其分析恢复复杂像差和高空间带宽产品复杂光场与na匹配和暗场照明的能力。然而,由于na匹配照明的波前曲率和兰伯LED光源的有限光束角度,其平面照明设置限制了其同时以高分辨率有效重建大视场的能力。在这里,我们介绍了一个为APIC量身定制的照明框架,由一个遥远的环形LED环和一个LED圆顶组成,可以用扩展的合成数值孔径重建更大的区域,从而提高分辨率。对于一组测量,我们的新原型,称为Dome-APIC,可以在450 μ m x 450 μ m的视场上使用10×/0.25 NA的物镜达到620nm的分辨率。
Dome-APIC illumination design for high space-bandwidth product analytic imaging.
Breaking the tradeoff between resolution and field-of-view, while obtaining distortion-free images, can be achieved through computational imaging techniques. A recent approach, Angular Ptychographic Imaging with Close-form method (APIC), has showcased its capability to analytically recover both intricate aberrations and high space-bandwidth product complex optical fields with NA-matching and darkfield illuminations. However, its flat illumination setup limits its ability to efficiently reconstruct a large field-of-view simultaneously with high resolution, owing to the curvature in the wavefront from NA-matching illuminations and the finite beam angle of the Lambertian LED light source. Here, we introduce an illumination framework tailored for APIC consisting of a distant annular LED ring and an LED dome that enables the reconstruction of a larger area with an extended synthetic numerical aperture, consequently enhancing resolution. For a single set of measurements, our new prototype, termed Dome-APIC can reach 620nm resolution with a 10×/0.25 NA objective lens over a field-of-view of 450 µm x 450 µm.
期刊介绍:
The journal''s scope encompasses fundamental research, technology development, biomedical studies and clinical applications. BOEx focuses on the leading edge topics in the field, including:
Tissue optics and spectroscopy
Novel microscopies
Optical coherence tomography
Diffuse and fluorescence tomography
Photoacoustic and multimodal imaging
Molecular imaging and therapies
Nanophotonic biosensing
Optical biophysics/photobiology
Microfluidic optical devices
Vision research.